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A0A0M5LYB0 · A0A0M5LYB0_9PSEU

Function

function

Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.
Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
K+ (UniProtKB | Rhea| CHEBI:29103 )

Note: Binds 1 potassium ion per subunit.
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site268(6S)-NADPHX (UniProtKB | ChEBI)
Binding site321(6S)-NADPHX (UniProtKB | ChEBI)
Binding site372(6S)-NADPHX (UniProtKB | ChEBI)
Binding site405-409AMP (UniProtKB | ChEBI)
Binding site434AMP (UniProtKB | ChEBI)
Binding site435(6S)-NADPHX (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionADP-dependent NAD(P)H-hydrate dehydratase activity
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Molecular FunctionNADHX epimerase activity
Molecular FunctionNADPHX epimerase activity
Biological Processmetabolite repair
Biological Processnicotinamide nucleotide metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    ADP-dependent (S)-NAD(P)H-hydrate dehydratase
  • EC number
  • Alternative names
    • ADP-dependent NAD(P)HX dehydratase

Gene names

    • Name
      nnrD
    • ORF names
      XF36_01445

Organism names

  • Taxonomic identifier
  • Strain
    • HH130629-09
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Pseudonocardiales > Pseudonocardiaceae > Pseudonocardia

Accessions

  • Primary accession
    A0A0M5LYB0

Proteomes

Interaction

Subunit

Homotetramer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain10-224YjeF N-terminal
Domain233-496YjeF C-terminal

Sequence similarities

Belongs to the NnrD/CARKD family.
In the C-terminal section; belongs to the NnrD/CARKD family.
In the N-terminal section; belongs to the NnrE/AIBP family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    503
  • Mass (Da)
    50,190
  • Last updated
    2015-12-09 v1
  • MD5 Checksum
    9C1DADDDB99C5913DF09E67BE036B471
MHGLHTADQVRAAEAAMMRTVGDGVLMRRAAAGLAHHVRAFLGATYGRRVVLLVGAGDNGGDALWAGVELRRRGAHVTAVLLAPDRAHPAGLAALRAARGRVLALHAPPVSGPGPAAAGALDTARALIAGADVVVDGIVGISGRGALRDPAPELVAAADGAGVPIAACDLPSGVDCDTGATDGPHVRAALTVTFGCRKPVHALAAPLCGPVRLVDFGLGPFLEGTEPHAWVLSDGDVGARWPVPGPDDDKYSQGVVGIAAGSATYPGAAVLAAGAAALATSGMVRFAGSAADEVRRHWPEIVATGDITDAGRTQAWAVGPGLGTGGQGLAVLEEALGREVPLCLDADAITLLTQHAHLRDRIHGEPVVLTPHAGEFARIAGEVGPDRIGAARRAAADLGVTVLLKGNATVVAAPDGRVLVDPAADSWAATAGSGDVLTGMIGALLAAGLDPWWAAGCATLVHARAATAAAREHGLPPVPAPASAMQAAIPVALRAVRAAAAPY

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP011868
EMBL· GenBank· DDBJ
ALE81957.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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