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A0A0H3NRI4 · A0A0H3NRI4_YERE1

Function

function

Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.

Catalytic activity

  • Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.
    EC:3.1.26.12 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per homotetramer.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site300Mg2+ (UniProtKB | ChEBI); catalytic
Binding site343Mg2+ (UniProtKB | ChEBI); catalytic
Binding site401Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site404Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytoplasmic side of plasma membrane
Molecular Functionmagnesium ion binding
Molecular Functionribonuclease E activity
Molecular FunctionRNA endonuclease activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological ProcessmRNA catabolic process
Biological ProcessrRNA processing
Biological ProcesstRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease E
  • EC number
  • Short names
    RNase E

Gene names

    • Name
      rne
    • Ordered locus names
      Y11_05201

Organism names

Accessions

  • Primary accession
    A0A0H3NRI4

Proteomes

Subcellular Location

Cytoplasm
Cell inner membrane
; Peripheral membrane protein

Keywords

Interaction

Subunit

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Within the RNA degradosome, RNase E assembles into a homotetramer formed by a dimer of dimers.

Family & Domains

Features

Showing features for domain, region, compositional bias.

Type
IDPosition(s)Description
Domain36-116S1 motif
Region401-404Required for zinc-mediated homotetramerization and catalytic activity
Region577-750Disordered
Compositional bias585-666Basic and acidic residues
Compositional bias667-682Polar residues
Compositional bias683-716Basic and acidic residues
Compositional bias790-809Polar residues
Region790-840Disordered
Region1103-1152Disordered

Sequence similarities

Belongs to the RNase E/G family. RNase E subfamily.
Belongs to the RNase E/G family. RNase G subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,152
  • Mass (Da)
    126,702
  • Last updated
    2015-09-16 v1
  • MD5 Checksum
    88AA305F77A2CE330F74AF5F0B5960B3
MLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKGKITRIEPSLEAAFVDYGAERHGFLPLKEISREYFPSNYSSHGRPNIKDVLREGQEVIVQVDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALSSLQLPDGMGLIVRTAGVGKSADALQWDLSFRLKHWDAIKKAAEGRPAPFLIHQESNVIVRAFRDYLRPDIGEILIDNPKVLELAKEHIAALGRPDFSSKIKLYSGEIPLFSHYQIESQIESAFQREVRLPSGGSIVIDTTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQLRLRDLGGLIVIDFIDMTPVRHQREVENRLRDAVRQDRARIQIGRISRFGLLEMSRQRLSPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTHEVHAIVPVQIASYLLNEKRESVNAIEKRQGGVRAVIVPNDQMQTPHYSVLRVRKGEEVPSLSYLLPQLHEAEMAQPLEEAAIERKRPEQPALATFSLPTEVPPESAPAAANVKPTAAPQAATAAVAEQPGFLSRLFSGLKGLFGGSTETEVKPVEVEKTETNENRRNDRRNPRRQNNGRKDRGDRTPREGRDNSSRDNNARDNSTRDNGNRDNANRDNSNREGRDDQRRNNRRPAQQATTPQAQTEVVEADKTQREEQPARRGDRQRRRPQDDKRQVSQDVKAKVDADIVTAVVEEAQPEQEERQQVMQRRQRRQLNQKVRIQSANDELNVLESSVSEPVSHITAPVVQEEVKLLPQASSQADDEASNDRNGNNENGMPRRSRRSPRHLRVSGQRRRRYRDERYPTQSAMPLAGAFASPEMASGKVWVRYPVAQPFEPAFVENPVEEQQPVEQVQEVTAVVEQVAVVAAPVVVAAVAETVLPTPAPQHKPGGSSSSAAAVPGRAPVVVETAIVETAIVETVTVAPIVETHAVEEPTQVTEIAPAAETITTVEPVIEDAVATETVVEATIEPVAVVEEATQELVVEETAVVVDDVTVAEEAVVEDVTVEEAVVEAPIVAETVVEDSVAEEATVTELPTDTAPVAVAPQQPVVAHPEGVLFKHYASAPMTKAPAPDYQPASPSQGHWERPAFDFAGKGSAGGHAAATKATAPATKPQSVE

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias585-666Basic and acidic residues
Compositional bias667-682Polar residues
Compositional bias683-716Basic and acidic residues
Compositional bias790-809Polar residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FR729477
EMBL· GenBank· DDBJ
CBY27661.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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