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A0A0E0TBJ5 · A0A0E0TBJ5_GEOS2

Function

function

Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O2 and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a central role in the inducible response to nitrosative stress.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
FAD (UniProtKB | Rhea| CHEBI:57692 )

Note: Binds 1 FAD per subunit.
heme b (UniProtKB | Rhea| CHEBI:60344 )

Note: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.

Features

Showing features for site, binding site, active site.

Type
IDPosition(s)Description
Site34Involved in heme-bound ligand stabilization and O-O bond activation
Site89Influences the redox potential of the prosthetic heme and FAD groups
Binding site90Fe (UniProtKB | ChEBI) of heme b (UniProtKB | ChEBI); proximal binding residue
Active site100Charge relay system
Active site142Charge relay system
Binding site195FAD (UniProtKB | ChEBI)
Binding site211-214FAD (UniProtKB | ChEBI)
Binding site281-286NADP+ (UniProtKB | ChEBI)
Site400Influences the redox potential of the prosthetic heme and FAD groups
Binding site401-404FAD (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionFAD binding
Molecular Functionheme binding
Molecular Functionmetal ion binding
Molecular Functionnitric oxide dioxygenase NAD(P)H activity
Molecular Functionoxygen binding
Molecular Functionoxygen carrier activity
Biological Processcellular response to nitrosative stress
Biological Processnitric oxide catabolic process
Biological Processresponse to toxic substance

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Flavohemoprotein
  • Alternative names
    • Flavohemoglobin
    • Hemoglobin-like protein
    • Nitric oxide dioxygenase
      (NO oxygenase
      ; NOD
      ) (EC:1.14.12.17
      ) . EC:1.14.12.17 (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      hmp
    • Ordered locus names
      GYMC52_1674

Organism names

Accessions

  • Primary accession
    A0A0E0TBJ5

Proteomes

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain10-143Globin
Region154-411Reductase
Domain157-268FAD-binding FR-type

Domain

Consists of two distinct domains; an N-terminal heme-containing oxygen-binding domain and a C-terminal reductase domain with binding sites for FAD and NAD(P)H.

Sequence similarities

Belongs to the globin family. Two-domain flavohemoproteins subfamily.
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    411
  • Mass (Da)
    46,086
  • Last updated
    2015-05-27 v1
  • MD5 Checksum
    ED3981F1E1F31A8E80939449ABAD154A
MTTTTKLHPKTIEIVKSTVPVLETHGEQITKRFYELMFSNHPELLNIFNHANQKQGRQQRALAAAVYAAARYIDQLDAILPVVRQIGHKHRSLGIKPEQYPIVGKHLLLAIKDVLGDAATDEVITAWAEAYEAIASVFIQVEKELYDEAAAKHGGWRDFRRFVVVKKVKESGVITSFYLEPEDGKAISDYLPGQYVSVKLSIPGETYTHIRQYSLSDAPGKGYYRISVKREAATADKPAGIVSNYLHDHVQEGDVLELSAPAGDFTLDLSKTTPVVFISGGVGITPLLSMAHTLAIRQPTRPAAFLHAALNGRVHAFDKELRMLAERPSFSYHICYESPSDEDRRHPHFGKEGRIDLAWMQSVIPTKDADFYFCGPVLFMKTVYRALKQWGVPEEHIHYEFFGPAGDLTKD

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP002442
EMBL· GenBank· DDBJ
ADU94109.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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