A0A077YBL0 · PDEG_PLAYE
- ProteincGMP-specific 3',5'-cyclic phosphodiesterase gamma
- GenePDEgamma
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids782 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Specifically hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes (Probable). Probably by regulating cGMP levels, required for sporozoite motility and invasion of the mosquito salivary glands (PubMed:25784701).
Catalytic activity
- 3',5'-cyclic GMP + H2O = GMP + H+
Cofactor
Note: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.
Pathway
Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 504 | Proton donor | |||
Binding site | 504-508 | a nucleoside 3',5'-cyclic phosphate (UniProtKB | ChEBI) | |||
Binding site | 508 | a divalent metal cation 1 (UniProtKB | ChEBI) | |||
Binding site | 544 | a divalent metal cation 1 (UniProtKB | ChEBI) | |||
Binding site | 545 | a divalent metal cation 1 (UniProtKB | ChEBI) | |||
Binding site | 545 | a divalent metal cation 2 (UniProtKB | ChEBI) | |||
Binding site | 545 | a nucleoside 3',5'-cyclic phosphate (UniProtKB | ChEBI) | |||
Binding site | 654 | a divalent metal cation 1 (UniProtKB | ChEBI) | |||
Binding site | 654 | a nucleoside 3',5'-cyclic phosphate (UniProtKB | ChEBI) | |||
Binding site | 706 | a nucleoside 3',5'-cyclic phosphate (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Molecular Function | calmodulin-activated 3',5'-cyclic-GMP phosphodiesterase activity | |
Molecular Function | calmodulin-activated dual specificity 3',5'-cyclic-GMP, 3',5'-cyclic-AMP phosphodiesterase activity | |
Molecular Function | metal ion binding | |
Biological Process | cGMP catabolic process | |
Biological Process | signal transduction |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namecGMP-specific 3',5'-cyclic phosphodiesterase gamma
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Sar > Alveolata > Apicomplexa > Aconoidasida > Haemosporida > Plasmodiidae > Plasmodium > Plasmodium (Vinckeia)
Accessions
- Primary accessionA0A077YBL0
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Multi-pass membrane protein
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Topological domain | 1-69 | Cytoplasmic | |||
Transmembrane | 70-90 | Helical | |||
Topological domain | 91-97 | Extracellular | |||
Transmembrane | 98-118 | Helical | |||
Topological domain | 119-135 | Cytoplasmic | |||
Transmembrane | 136-156 | Helical | |||
Topological domain | 157-181 | Extracellular | |||
Transmembrane | 182-202 | Helical | |||
Topological domain | 203-208 | Cytoplasmic | |||
Transmembrane | 209-229 | Helical | |||
Topological domain | 230-239 | Extracellular | |||
Transmembrane | 240-260 | Helical | |||
Topological domain | 261-782 | Cytoplasmic | |||
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
During infection of Swiss Webster mice, lack of PDEgamma reduces peak blood stage parasitemia (PubMed:25784701).
In the mosquito, development of oocyst sporozoites is not affected (PubMed:25784701).
However, sporozoites released from the oocyst have higher cGMP levels, are immotile and fail to invade the salivary glands and, thus, parasite transmission to the mammalian host is impaired (PubMed:25784701).
In addition, in sporozoites, mRNA levels of PDEbeta and PDEdelta are up-regulated and several transcripts encoding proteins involved in sporozoite invasion of mosquito salivary glands and sporozoite infectivity such as TRAP and CSP are down-regulated (PubMed:25784701).
In the mosquito, development of oocyst sporozoites is not affected (PubMed:25784701).
However, sporozoites released from the oocyst have higher cGMP levels, are immotile and fail to invade the salivary glands and, thus, parasite transmission to the mammalian host is impaired (PubMed:25784701).
In addition, in sporozoites, mRNA levels of PDEbeta and PDEdelta are up-regulated and several transcripts encoding proteins involved in sporozoite invasion of mosquito salivary glands and sporozoite infectivity such as TRAP and CSP are down-regulated (PubMed:25784701).
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000452649 | 1-782 | cGMP-specific 3',5'-cyclic phosphodiesterase gamma | ||
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 376-396 | Disordered | |||
Domain | 423-751 | PDEase | |||
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length782
- Mass (Da)92,307
- Last updated2014-10-29 v1
- MD5 Checksum1BEF22E18501300AB21B4912349C9F2B
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
LK934642 EMBL· GenBank· DDBJ | CDU20569.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LM993668 EMBL· GenBank· DDBJ | VTZ81530.1 EMBL· GenBank· DDBJ | Genomic DNA |