Essential maintenance is planned to begin on Fri Jan 24 2025. The website may be temporarily unavailable. Please use our fallback: https://wwwdev.ebi.ac.uk/uniprot/front-end/fallback/ in case of any outage.

A0A077YBL0 · PDEG_PLAYE

  • Protein
    cGMP-specific 3',5'-cyclic phosphodiesterase gamma
  • Gene
    PDEgamma
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    4/5

Function

function

Specifically hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes (Probable). Probably by regulating cGMP levels, required for sporozoite motility and invasion of the mosquito salivary glands (PubMed:25784701).

Catalytic activity

Cofactor

a divalent metal cation (UniProtKB | Rhea| CHEBI:60240 )

Note: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.

Pathway

Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site504Proton donor
Binding site504-508a nucleoside 3',5'-cyclic phosphate (UniProtKB | ChEBI)
Binding site508a divalent metal cation 1 (UniProtKB | ChEBI)
Binding site544a divalent metal cation 1 (UniProtKB | ChEBI)
Binding site545a divalent metal cation 1 (UniProtKB | ChEBI)
Binding site545a divalent metal cation 2 (UniProtKB | ChEBI)
Binding site545a nucleoside 3',5'-cyclic phosphate (UniProtKB | ChEBI)
Binding site654a divalent metal cation 1 (UniProtKB | ChEBI)
Binding site654a nucleoside 3',5'-cyclic phosphate (UniProtKB | ChEBI)
Binding site706a nucleoside 3',5'-cyclic phosphate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum membrane
Molecular Functioncalmodulin-activated 3',5'-cyclic-GMP phosphodiesterase activity
Molecular Functioncalmodulin-activated dual specificity 3',5'-cyclic-GMP, 3',5'-cyclic-AMP phosphodiesterase activity
Molecular Functionmetal ion binding
Biological ProcesscGMP catabolic process
Biological Processsignal transduction

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    cGMP-specific 3',5'-cyclic phosphodiesterase gamma
  • EC number

Gene names

    • Name
      PDEgamma
    • ORF names
      PY17X_1421600

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • 17X
  • Taxonomic lineage
    Eukaryota > Sar > Alveolata > Apicomplexa > Aconoidasida > Haemosporida > Plasmodiidae > Plasmodium > Plasmodium (Vinckeia)

Accessions

  • Primary accession
    A0A077YBL0

Proteomes

Organism-specific databases

Subcellular Location

Membrane
; Multi-pass membrane protein
Endoplasmic reticulum membrane
; Multi-pass membrane protein
Note: Intracellular localization in blood stage forms and in sporozoites (PubMed:25784701).
Partially, localizes to the endoplasmic reticulum in blood stage forms (PubMed:25784701).

Features

Showing features for topological domain, transmembrane.

Type
IDPosition(s)Description
Topological domain1-69Cytoplasmic
Transmembrane70-90Helical
Topological domain91-97Extracellular
Transmembrane98-118Helical
Topological domain119-135Cytoplasmic
Transmembrane136-156Helical
Topological domain157-181Extracellular
Transmembrane182-202Helical
Topological domain203-208Cytoplasmic
Transmembrane209-229Helical
Topological domain230-239Extracellular
Transmembrane240-260Helical
Topological domain261-782Cytoplasmic

Keywords

Phenotypes & Variants

Disruption phenotype

During infection of Swiss Webster mice, lack of PDEgamma reduces peak blood stage parasitemia (PubMed:25784701).
In the mosquito, development of oocyst sporozoites is not affected (PubMed:25784701).
However, sporozoites released from the oocyst have higher cGMP levels, are immotile and fail to invade the salivary glands and, thus, parasite transmission to the mammalian host is impaired (PubMed:25784701).
In addition, in sporozoites, mRNA levels of PDEbeta and PDEdelta are up-regulated and several transcripts encoding proteins involved in sporozoite invasion of mosquito salivary glands and sporozoite infectivity such as TRAP and CSP are down-regulated (PubMed:25784701).

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004526491-782cGMP-specific 3',5'-cyclic phosphodiesterase gamma

Expression

Developmental stage

Expressed during the asexual blood stage (PubMed:25784701).
In the mosquito vector, expressed in oocyst sporozoites, and salivary gland sporozoites (PubMed:25784701).

Family & Domains

Features

Showing features for region, domain.

Type
IDPosition(s)Description
Region376-396Disordered
Domain423-751PDEase

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    782
  • Mass (Da)
    92,307
  • Last updated
    2014-10-29 v1
  • MD5 Checksum
    1BEF22E18501300AB21B4912349C9F2B
MKHMFKNILFHKKGKHDKNDAIKKAFSLFSVPSNENERIIKFWPLKFKEKDEETLYIIKLCDNMYSKKYVILVSHLISLLLMYSVCLIVGNINDLFSVLKLTYILLHTFTAINIILILTLHATHYVEMFKSIKGEIFIFYIMMIFVIWCSWLFILFNNIKDLLPIVVNVNNFLYATYANNKINIVLGFFAYLPIFYLITIIPCRICYSCAFDILFFIMKVAIFSVYYLITMKSYILTDNIFMIISALVGSLFIFVIRYIIEIQRRLSFHNWNKQTKQIIKLKKTLKEEKQKLSTTNIEEIYNLINDSIGNYYNENKKQKETDWSIVNNLEKILNILKEDNLFSPDLKTINKKNYNHIYGYIMDLKKQKEIINDKIGSKEEPEAESESECVDESKEGSQIESIFESISDVKQKKKSDLAYTSSYEEKENEILKYDFNMNMDKENISIDIWNTKFLDRKSPNYDAFIKIGYILLNKYYISNQNISVKILYSLLYEMKKGYNDVPYHNSIHAAMVTKHCSILITSLDTVNILKDNEMAAFLISALGHDIGHFGRTNMFLKNCSNFLRIIYNDKSILENYHCSYLFNILSKEEHNIFKKEDLKTLTNLRQLIIEVILATDMSKHIKILAQFRIKSIKIKSYIEKNIILCLKMIIKAADLSHNCVDWSEHYLWVKRLVNEFYSEGDDLLERGFELNPLFDRKAHNNFIQIQRTFLRELVLPLISSLKTLDTSTITQLMLSHVKRNYSKWTKIEKDETKKEKYLNELLTDVPNSWKIVYAPNLNIYKL

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LK934642
EMBL· GenBank· DDBJ
CDU20569.1
EMBL· GenBank· DDBJ
Genomic DNA
LM993668
EMBL· GenBank· DDBJ
VTZ81530.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help