A0A067XMP0 · PTAE_PESFW

Function

function

Oxidoreductase; part of the gene cluster that mediates the biosynthesis of pestheic acid, a diphenyl ether which is a biosynthetic precursor of the unique chloropupukeananes (PubMed:24302702).
The biosynthesis initiates from condensation of acetate and malonate units catalyzed by the non-reducing PKS ptaA (PubMed:24302702).
As the ptaA protein is TE/CLC domain-deficient, hydrolysis and Claisen cyclization of the polyketide could be catalyzed by ptaB containing a beta-lactamase domain (PubMed:24302702).
The ptaB protein might hydrolyze the thioester bond between the ACP of ptaA and the intermediate to release atrochrysone carboxylic acid, which is spontaneously dehydrated to form endocrocin anthrone (PubMed:24302702).
Endocrocin anthrone is then converted to endocrocin, catalyzed by the anthrone oxygenase ptaC (PubMed:24302702).
Spontaneous decarboxylation of endocrocin occurs to generate emodin (PubMed:24302702).
An O-methyltransferase (ptaH or ptaI) could methylate emodin to form physcion (PubMed:24302702).
PtaJ could then catalyze the oxidative cleavage of physcion, and rotation of the intermediate could then afford desmethylisosulochrin (PubMed:24302702).
PtaF, a putative NADH-dependent oxidoreductase, might also participate in the oxidative cleavage step (PubMed:24302702).
Desmethylisosulochrin is then transformed by another O-methyltransferase (ptaH or ptaI) to form isosulochrin (PubMed:24302702).
Chlorination of isosulochrin by ptaM in the cyclohexadienone B ring then produces chloroisosulochrin (PubMed:24302702).
PtaE is responsible for the oxidative coupling reactions of both benzophenones isosulouchrin and chloroisosulochrin to RES-1214-1 and pestheic acid respectively, regardless of chlorination

Pathway

Secondary metabolite biosynthesis.

GO annotations

AspectTerm
Molecular Functioncopper ion binding
Molecular Functionoxidoreductase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Oxidoreductase ptaE
  • EC number
  • Alternative names
    • Dihydrogeodin oxidase
      (DHGO
      )
    • Pestheic acid biosynthesis cluster protein E

Gene names

    • Name
      ptaE
    • ORF names
      PFICI_10828

Organism names

Accessions

  • Primary accession
    A0A067XMP0
  • Secondary accessions
    • W3WT07

Proteomes

Phenotypes & Variants

Disruption phenotype

Abolishes the production of pestheic acid and RES-1214-1, but accumulates isosulochrin and chloroisosulochrin (PubMed:24302702).

PTM/Processing

Features

Showing features for signal, chain, glycosylation.

Type
IDPosition(s)Description
Signal1-20
ChainPRO_000044305021-610Oxidoreductase ptaE
Glycosylation105N-linked (GlcNAc...) asparagine
Glycosylation111N-linked (GlcNAc...) asparagine
Glycosylation262N-linked (GlcNAc...) asparagine
Glycosylation277N-linked (GlcNAc...) asparagine
Glycosylation330N-linked (GlcNAc...) asparagine
Glycosylation356N-linked (GlcNAc...) asparagine
Glycosylation401N-linked (GlcNAc...) asparagine
Glycosylation409N-linked (GlcNAc...) asparagine
Glycosylation427N-linked (GlcNAc...) asparagine
Glycosylation602N-linked (GlcNAc...) asparagine

Keywords

PTM databases

Expression

Induction

The cluster is expressed in rice fermentation medium (PubMed:25623211).
Expression is correlated with the production of pestheic acid (PubMed:24302702).
Three regulators are located in the cluster (ptaR1, ptaR2 and ptaR3), suggesting that the production of pestheic acid is controlled by a complex regulatory mechanism (PubMed:24302702).

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain67-181Plastocyanin-like 1
Domain191-344Plastocyanin-like 2
Domain425-568Plastocyanin-like 3

Sequence similarities

Belongs to the multicopper oxidase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    610
  • Mass (Da)
    68,758
  • Last updated
    2018-01-31 v2
  • Checksum
    F5F87BE6DDD38970
MFQSILFLAFYGRPVFGSAAARDYACVNTAESRDCWKDGFNIETDYYGKEEAPEGKLVEYELTLSQQIISPDGYEMLGMVVNGQYPGPTIEADWGDTLRITVKNNFTENYNGTAVHWHGIRQKETNWLDGVPGVTQCPITPGDSQVYEFRVTQYGTSWYHSHYSLQYSNGAYGPIVIHGPSSANWDVDLGPWLLSDWYHDDAFALDHVGITTNRAAIPKSSLINGKGYYECDPTNDAKCTGTRDYYEVVLKQGTKYKFGIINTSTILTYTFWIDGHNFTIIAIDFVPIEPLTVDTLNVGIGQRYEIIIETNPDFDDDSSFWMHAQYCFINQTDIVDDKVGIVRYESAGSSDPPYINKSDYHLNFGCADPKPESLVPILKQQVGAQANPLAAEDYFRVGLGNFTWPDATNSTGSVFLWFLQKLPLYVNWSEPSVKKLTIDETADFPPNSRPIELDYETGQWVYFVIESDWDPAGAVDQYGQEIRVEPSVHPFHLHGHDFLILAQGLGKFTSDIQPNLDNPPRRDTVDVEPLGYVWIAFQIDNPGAWLFHCHIAFHSSDGIAIQFLEQPSKLKPIMEEAGVLGDFADRCNKWDDWYQAVNIPHNATQADSGV

Sequence caution

The sequence AGO59042.1 differs from that shown. Reason: Erroneous gene model prediction

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KC145148
EMBL· GenBank· DDBJ
AGO59042.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
KI912116
EMBL· GenBank· DDBJ
ETS76954.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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