A0A066SS77 · A0A066SS77_ECOLX
- ProteinQuinate/shikimate dehydrogenase
- GeneydiB
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids288 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
The actual biological function of YdiB remains unclear, nor is it known whether 3-dehydroshikimate or quinate represents the natural substrate. Catalyzes the reversible NAD-dependent reduction of both 3-dehydroshikimate (DHSA) and 3-dehydroquinate to yield shikimate (SA) and quinate, respectively. It can use both NAD or NADP for catalysis, however it has higher catalytic efficiency with NAD.
Catalytic activity
- L-quinate + NAD+ = 3-dehydroquinate + NADH + H+
Pathway
Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 71 | substrate | |||
Binding site | 107 | substrate | |||
Binding site | 132-135 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 155-158 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 205 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 232-235 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 255 | NAD+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | NADP binding | |
Molecular Function | quinate 3-dehydrogenase (NAD+) activity | |
Molecular Function | quinate 3-dehydrogenase (NADP+) activity | |
Molecular Function | shikimate 3-dehydrogenase (NAD+) activity | |
Molecular Function | shikimate 3-dehydrogenase (NADP+) activity | |
Biological Process | amino acid biosynthetic process | |
Biological Process | aromatic amino acid family biosynthetic process | |
Biological Process | chorismate biosynthetic process | |
Biological Process | shikimate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameQuinate/shikimate dehydrogenase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionA0A066SS77
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Sequence
- Sequence statusComplete
- Length288
- Mass (Da)31,229
- Last updated2014-09-03 v1
- MD5 Checksum8E5691E64A8DB12E1A1B740A3BB3C27B
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
ABONVU020000002 EMBL· GenBank· DDBJ | EMJ5252613.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DABBJX010000023 EMBL· GenBank· DDBJ | HAH4525949.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DABCJL010000002 EMBL· GenBank· DDBJ | HAH7767610.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
SCJN01000010 EMBL· GenBank· DDBJ | RXD17875.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP107128 EMBL· GenBank· DDBJ | WLM98268.1 EMBL· GenBank· DDBJ | Genomic DNA |