W7N6P0 · FUS2_GIBM7

Function

function

20-hydroxy-prefusarin hydrolase; part of the gene cluster that mediates the biosynthesis of the mycotoxin fusarin C (PubMed:17121404, PubMed:22652150).
Within the cluster, FUS1, FUS2, FUS8 and FUS9 are sufficient for fusarin production (By similarity).
The roles of the other FUS members are yet undetermined (By similarity).
The fusarin C synthetase FUS1 is responsible for the condensation of one acetyl-coenzyme A (CoA) unit with six malonyl-CoA units and the amide linkage of the arising heptaketide and homoserine, subsequently releasing the first intermediate, prefusarin, as an alcohol with an open ring structure (PubMed:17121404).
The cytochrome P450 monooxygenase FUS8 participates in multiple oxidation processes at carbon C-20 and is able to use the FUS1 product as substrate, resulting in formation of 20-hydroxy-prefusarin (By similarity).
This reaction seems to be essential before the 2-pyrrolidone ring closure can be catalyzed by FUS2, generating 20-hydroxy-fusarin (By similarity).
FUS8 is able to further oxidizes carbon C-20 after ring closure, resulting in the formation of carboxy-fusarin C (By similarity).
As the last step, FUS9 methylates the hydroxyl group at C-21 to generate fusarin C (By similarity).
Fusarin C can then rearrange to epi-fusarin C, the (z)-isomers, and fusarin A and fusarin D (By similarity).

Pathway

Mycotoxin biosynthesis.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site238

GO annotations

AspectTerm
Molecular Functionhydrolase activity

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    20-hydroxy-prefusarin hydrolase FUS2
  • EC number
  • Alternative names
    • Fusarin biosynthesis protein 2

Gene names

    • Name
      FUS2
    • ORF names
      FVEG_11085

Organism names

Accessions

  • Primary accession
    W7N6P0

Proteomes

Organism-specific databases

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004373581-41920-hydroxy-prefusarin hydrolase FUS2

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    419
  • Mass (Da)
    46,314
  • Last updated
    2014-04-16 v1
  • Checksum
    3F1059F1C0EAF61B
MHKVFASDFFNFEFLRLLGTVPFQGAEVGECLTTAGRIKDGDPESWYRAWRDQAEKAQALAEEAAAVGDRTGACWAYIRAANYWRASEFLLHCTPNDPRILAASKASVNAFDKGWVLLDATVKSFEIPYDKDIKLPGRLYLPAPHHRLPGKIPVVLQTGGFDSTQEELYFYGAAGALPRGYAVFSFDGPGQGLPLRVGKLKLRTDWEYVVSQVLDFVTDDIAPEYDLDLERLAIFGASLGGYLSLRAAVDPRIKACISCDGPLDLFEITRSRMPSWFINGWLSGWVSDGLFNWVVDALTAVNFQIAWEFGHGKWVFGVETPADVLRVMQQISLKGGYLSKIKCPTLITGAADSFYFTPDINANPIFEGLTALGSNEKHLWIGKGVEGGGLQAKIGALAVVHHKMFTWLDSTFAIKRDVL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CM000586
EMBL· GenBank· DDBJ
EWG52307.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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