W7N6P0 · FUS2_GIBM7
- Protein20-hydroxy-prefusarin hydrolase FUS2
- GeneFUS2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids419 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
20-hydroxy-prefusarin hydrolase; part of the gene cluster that mediates the biosynthesis of the mycotoxin fusarin C (PubMed:17121404, PubMed:22652150).
Within the cluster, FUS1, FUS2, FUS8 and FUS9 are sufficient for fusarin production (By similarity).
The roles of the other FUS members are yet undetermined (By similarity).
The fusarin C synthetase FUS1 is responsible for the condensation of one acetyl-coenzyme A (CoA) unit with six malonyl-CoA units and the amide linkage of the arising heptaketide and homoserine, subsequently releasing the first intermediate, prefusarin, as an alcohol with an open ring structure (PubMed:17121404).
The cytochrome P450 monooxygenase FUS8 participates in multiple oxidation processes at carbon C-20 and is able to use the FUS1 product as substrate, resulting in formation of 20-hydroxy-prefusarin (By similarity).
This reaction seems to be essential before the 2-pyrrolidone ring closure can be catalyzed by FUS2, generating 20-hydroxy-fusarin (By similarity).
FUS8 is able to further oxidizes carbon C-20 after ring closure, resulting in the formation of carboxy-fusarin C (By similarity).
As the last step, FUS9 methylates the hydroxyl group at C-21 to generate fusarin C (By similarity).
Fusarin C can then rearrange to epi-fusarin C, the (z)-isomers, and fusarin A and fusarin D (By similarity).
Within the cluster, FUS1, FUS2, FUS8 and FUS9 are sufficient for fusarin production (By similarity).
The roles of the other FUS members are yet undetermined (By similarity).
The fusarin C synthetase FUS1 is responsible for the condensation of one acetyl-coenzyme A (CoA) unit with six malonyl-CoA units and the amide linkage of the arising heptaketide and homoserine, subsequently releasing the first intermediate, prefusarin, as an alcohol with an open ring structure (PubMed:17121404).
The cytochrome P450 monooxygenase FUS8 participates in multiple oxidation processes at carbon C-20 and is able to use the FUS1 product as substrate, resulting in formation of 20-hydroxy-prefusarin (By similarity).
This reaction seems to be essential before the 2-pyrrolidone ring closure can be catalyzed by FUS2, generating 20-hydroxy-fusarin (By similarity).
FUS8 is able to further oxidizes carbon C-20 after ring closure, resulting in the formation of carboxy-fusarin C (By similarity).
As the last step, FUS9 methylates the hydroxyl group at C-21 to generate fusarin C (By similarity).
Fusarin C can then rearrange to epi-fusarin C, the (z)-isomers, and fusarin A and fusarin D (By similarity).
Pathway
Mycotoxin biosynthesis.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 238 | |||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | hydrolase activity |
Keywords
- Molecular function
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended name20-hydroxy-prefusarin hydrolase FUS2
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Hypocreomycetidae > Hypocreales > Nectriaceae > Fusarium > Fusarium fujikuroi species complex
Accessions
- Primary accessionW7N6P0
Proteomes
Organism-specific databases
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000437358 | 1-419 | 20-hydroxy-prefusarin hydrolase FUS2 | |||
Sequence: MHKVFASDFFNFEFLRLLGTVPFQGAEVGECLTTAGRIKDGDPESWYRAWRDQAEKAQALAEEAAAVGDRTGACWAYIRAANYWRASEFLLHCTPNDPRILAASKASVNAFDKGWVLLDATVKSFEIPYDKDIKLPGRLYLPAPHHRLPGKIPVVLQTGGFDSTQEELYFYGAAGALPRGYAVFSFDGPGQGLPLRVGKLKLRTDWEYVVSQVLDFVTDDIAPEYDLDLERLAIFGASLGGYLSLRAAVDPRIKACISCDGPLDLFEITRSRMPSWFINGWLSGWVSDGLFNWVVDALTAVNFQIAWEFGHGKWVFGVETPADVLRVMQQISLKGGYLSKIKCPTLITGAADSFYFTPDINANPIFEGLTALGSNEKHLWIGKGVEGGGLQAKIGALAVVHHKMFTWLDSTFAIKRDVL |
Structure
Sequence
- Sequence statusComplete
- Length419
- Mass (Da)46,314
- Last updated2014-04-16 v1
- Checksum3F1059F1C0EAF61B
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CM000586 EMBL· GenBank· DDBJ | EWG52307.1 EMBL· GenBank· DDBJ | Genomic DNA |