S6D3A7 · NDB4N_TITSE

Function

function

Antimicrobial peptide (PubMed:23770440, PubMed:27567704, PubMed:27917162).
Has a high antibacterial activity against the Gram-positive bacterium S.aureus (MIC=5-17.30 uM), the methicillin-resistant S.aureus (MRSA) (MIC=17.30 uM), and E.faecalis (MIC=69.23 uM) (PubMed:23770440, PubMed:27567704).
Has antifungal activity against Candida spp. and one Cryptococcus neoformans strains with MICs values ranging from 6.25 to 100 uM (PubMed:23770440, PubMed:27567704, PubMed:27917162).
Shows also an inhibitory activity on C.albicans biofilms at high concentrations (PubMed:27917162).
Has a moderate hemolytic potency (18% at 20 uM) (PubMed:23770440, PubMed:27567704).
Also inhibits the growth of the five human cancer cell lines tested (the squamous carcinoma cell line H157 (IC50=4.1 uM), the lung adenocarcinoma cell line H838 (11.0 uM), the breast carcinoma cell line MCF-7 (6.4 uM), the androgen-independent prostate adenocarcinoma cell line PC3 (13.3 uM) and the glioblastoma cell line U251-MG (15.4 uM)) (PubMed:23770440).
In the model of polymicrobial sepsis, it exhibits an antibiotic effect, reducing the levels of microorganisms in the infectious focus and the inflammatory responses in the lung and cecum of septic animals (PubMed:27567704).

Miscellaneous

The primary structure of the mature peptide is identical to that of NDBP4.23-like from Tityus costatus (AC Q5G8B5).
Negative results: does not inhibit the Gram-negative bacterium E.coli (PubMed:23770440).
Does not show antifungal activity against Candida glabrata (ATCC90030) (MIC>400 uM) (PubMed:27917162).

pH Dependence

Stable to pH variation.

GO annotations

AspectTerm
Cellular Componentextracellular region
Biological Processdefense response to bacterium
Biological Processdefense response to fungus
Biological Processkilling of cells of another organism

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Antimicrobial peptide TsAP-2
  • Alternative names
    • Non-disulfide-bridged peptide 4.23
      (NDBP-4.23
      )

Organism names

Accessions

  • Primary accession
    S6D3A7

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis29Important increase of antimicrobial and hemolytic activities; when associated wiTH 32-K-K-33 and K-36.
Mutagenesis32-33Important increase of antimicrobial and hemolytic activities; when associated with K-29 and K-36.
Mutagenesis36Important increase of antimicrobial and hemolytic activities; when associated with K-29 and 32-K-K-33.

PTM/Processing

Features

Showing features for signal, peptide, modified residue, propeptide.

TypeIDPosition(s)Description
Signal1-22
PeptidePRO_500115557323-39Antimicrobial peptide TsAP-2
Modified residue39Lysine amide
PropeptidePRO_500115557445-73

Keywords

Expression

Tissue specificity

Expressed by the venom gland.

Family & Domains

Sequence similarities

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    73
  • Mass (Da)
    8,391
  • Last updated
    2013-10-16 v1
  • Checksum
    8E0E39B93B61F561
MQIKHLITIFFLVLIVADHCHAFLGMIPGLIGGLISAFKGRRKREITSQIEQYRNLQKREAELENLLANLPVY

Mass Spectrometry

Molecular mass is 1,733.32 Da. Determined by Electrospray.

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
HF677517
EMBL· GenBank· DDBJ
CCQ98792.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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