Q9UYX0 · TDH_PYRAB
- ProteinL-threonine 3-dehydrogenase
- Genetdh
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids348 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the NAD+-dependent oxidation of L-threonine to 2-amino-3-ketobutyrate.
Catalytic activity
- L-threonine + NAD+ = (2S)-2-amino-3-oxobutanoate + H+ + NADH
Cofactor
Note: Binds 2 Zn2+ ions per subunit.
Pathway
Amino-acid degradation; L-threonine degradation via oxydo-reductase pathway; glycine from L-threonine: step 1/2.
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 42 | Zn2+ 1 (UniProtKB | ChEBI); catalytic | ||||
Sequence: C | ||||||
Active site | 44 | Charge relay system | ||||
Sequence: T | ||||||
Active site | 47 | Charge relay system | ||||
Sequence: H | ||||||
Binding site | 67 | Zn2+ 1 (UniProtKB | ChEBI); catalytic | ||||
Sequence: H | ||||||
Binding site | 68 | Zn2+ 1 (UniProtKB | ChEBI); catalytic | ||||
Sequence: E | ||||||
Binding site | 97 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 100 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 103 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 111 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Site | 152 | Important for catalytic activity for the proton relay mechanism but does not participate directly in the coordination of zinc atom | ||||
Sequence: E | ||||||
Binding site | 179 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Binding site | 199 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 204 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 266-268 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: LGL | ||||||
Binding site | 291-292 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: IT |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | adenyl nucleotide binding | |
Molecular Function | anion binding | |
Molecular Function | L-threonine 3-dehydrogenase activity | |
Molecular Function | zinc ion binding | |
Biological Process | L-threonine catabolic process to glycine | |
Biological Process | protein tetramerization |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameL-threonine 3-dehydrogenase
- EC number
- Short namesTDH
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Thermococci > Thermococcales > Thermococcaceae > Pyrococcus
Accessions
- Primary accessionQ9UYX0
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000160875 | 1-348 | L-threonine 3-dehydrogenase | |||
Sequence: MSEKMVAIMKTKPAYGAELVEVDVPKPGPGEVLIKVIATSICGTDLHIYEWNEWAQSRIKPPQIMGHEVAGEVVEVGPGVEGIEVGDYVSVETHIVCGKCYACRRGQYHVCQNTKIFGVDTDGVFAEYAVVPAQNVWKNPKSIPPEYATLQEPLGNAVDTVLAGPISGKSVLITGAGPLGLLGIAVAKASGAYPVIVSEPSDFRRELAKKVGADYVINPFEEDVVKEVMDITDGNGVDVFLEFSGAPKALEQGLQAVTPAGRVSLLGLYPGKVSIDFNNLIIFKALTVYGITGRHLWETWYTVSRLLQSGKLNLDPIITHKYKGFDKYEEAFELMRAGKTGKVVFMLK |
Interaction
Structure
Sequence
- Sequence statusComplete
- Length348
- Mass (Da)37,744
- Last updated2000-05-01 v1
- Checksum91A8ED13DC4B7AD8
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ248287 EMBL· GenBank· DDBJ | CAB50292.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
HE613800 EMBL· GenBank· DDBJ | CCE70830.1 EMBL· GenBank· DDBJ | Genomic DNA |