Q9SDZ9 · PPAF2_IPOBA
- ProteinPurple acid phosphatase 2
- GenePAP2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids465 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
Catalytic activity
- a phosphate monoester + H2O = an alcohol + phosphate
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 Fe cation per subunit.
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )
Cu2+ (UniProtKB | Rhea| CHEBI:29036 )
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )
Note: Binds 1 Mn2+ ion per subunit. Can also use Zn2+, Cu2+ and Mg2+ ions.
Absorption
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
95 μM | p-NPP | 4.9 | 25 | |||
120 μM | ATP | 4.9 | 25 | |||
180 μM | ADP | 4.9 | 25 | |||
360 μM | AMP | 4.9 | 25 | |||
75 μM | pyrophosphate | 4.9 | 25 | |||
490 μM | beta-glycerophosphate | 4.9 | 25 |
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 164 | Fe cation (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 193 | Fe cation (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 193 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 196 | Fe cation (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 230 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 230 | substrate | ||||
Sequence: N | ||||||
Binding site | 315 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Active site | 325 | Proton donor | ||||
Sequence: H | ||||||
Binding site | 352 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 352-354 | substrate | ||||
Sequence: HVH | ||||||
Binding site | 354 | Fe cation (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | acid phosphatase activity | |
Molecular Function | metal ion binding |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePurple acid phosphatase 2
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Solanales > Convolvulaceae > Ipomoeeae > Ipomoea
Accessions
- Primary accessionQ9SDZ9
Subcellular Location
PTM/Processing
Features
Showing features for signal, chain, glycosylation, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-32 | |||||
Sequence: MGASRTGCYLLAVVLAAVMNAAIAGITSSFIR | ||||||
Chain | PRO_5000057352 | 33-465 | Purple acid phosphatase 2 | |||
Sequence: KVEKTVDMPLDSDVFRVPPGYNAPQQVHITQGDHVGKAMIVSWVTVDEPGSSKVVYWSENSQHKKVARGNIRTYTYFNYTSGYIHHCTIRNLEYNTKYYYEVGIGNTTRSFWFTTPPEVGPDVPYTFGLIGDLGQSFDSNRTLTHYERNPIKGQAVLFVGDLSYADNYPNHDNVRWDTWGRFVERSTAYQPWIWTAGNHEIDFAPEIGETKPFKPFTKRYHVPYKASGSTETFWYPIKRASAYIIVLSSYSAYGKYTPQYKWLEEELPKVNRTETPWLIVLMHSPWYNSYNYHYMEGETMRVMYEPWFVQHKVDLVFAGHVHAYERSERVSNVAYDIVNGKCTPVRDQSAPVYITIGDGGNLEGLATNMTDPQPEYSAFREASFGHATLDIKNRTHAYYSWHRNQDGYAVEADSMWVSNRFWHPVDDSTTTKL | ||||||
Glycosylation | 110 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 138 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 172 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 303 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 374 | Interchain | ||||
Sequence: C | ||||||
Glycosylation | 400 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 425 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
PTM databases
Interaction
Subunit
Homodimer; disulfide-linked.
Structure
Family & Domains
Sequence similarities
Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length465
- Mass (Da)53,401
- Last updated2000-05-01 v1
- Checksum49C0DCBA1CD8DF72