Q8WV22 · NSE1_HUMAN

  • Protein
    Non-structural maintenance of chromosomes element 1 homolog
  • Gene
    NSMCE1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

RING-type zinc finger-containing E3 ubiquitin ligase that assembles with melanoma antigen protein (MAGE) to catalyze the direct transfer of ubiquitin from E2 ubiquitin-conjugating enzyme to a specific substrate. Within MAGE-RING ubiquitin ligase complex, MAGE stimulates and specifies ubiquitin ligase activity likely through recruitment and/or stabilization of the E2 ubiquitin-conjugating enzyme at the E3:substrate complex. Involved in maintenance of genome integrity, DNA damage response and DNA repair (PubMed:20864041, PubMed:29225034).
NSMCE3/MAGEG1 and NSMCE1 ubiquitin ligase are components of SMC5-SMC6 complex and may positively regulate homologous recombination-mediated DNA repair (PubMed:18086888).
MAGEF1-NSMCE1 ubiquitin ligase promotes proteasomal degradation of MMS19, a key component of the cytosolic iron-sulfur protein assembly (CIA) machinery. Down-regulation of MMS19 impairs the activity of several DNA repair and metabolism enzymes such as ERCC2/XPD, FANCJ, RTEL1 and POLD1 that require iron-sulfur clusters as cofactors (PubMed:29225034).

Catalytic activity

  • S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.
    EC:2.3.2.27 (UniProtKB | ENZYME | Rhea)

GO annotations

AspectTerm
Cellular Componentchromosome, centromeric region
Cellular Componentchromosome, telomeric region
Cellular Componentintracellular membrane-bounded organelle
Cellular Componentmitotic spindle pole
Cellular Componentnucleoplasm
Cellular Componentnucleus
Cellular ComponentSmc5-Smc6 complex
Molecular Functionmetal ion binding
Molecular Functionprotein dimerization activity
Molecular Functionubiquitin protein ligase activity
Molecular Functionubiquitin-protein transferase activity
Biological Processchromatin looping
Biological ProcessDNA damage response
Biological Processdouble-strand break repair via homologous recombination
Biological Processprotein sumoylation
Biological Processregulation of telomere maintenance

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Non-structural maintenance of chromosomes element 1 homolog
  • EC number
  • Short names
    Non-SMC element 1 homolog

Gene names

    • Name
      NSMCE1
    • ORF names
      HSPC333, HSPC337

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo

Accessions

  • Primary accession
    Q8WV22
  • Secondary accessions
    • D3DWF6
    • Q9P045
    • Q9P049

Proteomes

Organism-specific databases

Disease & Variants

Features

Showing features for natural variant.

TypeIDPosition(s)Description
Natural variantVAR_02982238in dbSNP:rs7195194
Natural variantVAR_02982347in dbSNP:rs17856580

Variants

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The viewer provides 294 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

Organism-specific databases

Miscellaneous

Genetic variation databases

PTM/Processing

Features

Showing features for chain, modified residue (large scale data), modified residue.

Type
IDPosition(s)Source
Description
ChainPRO_00002709441-266UniProtNon-structural maintenance of chromosomes element 1 homolog
Modified residue (large scale data)241PTMeXchangeSumoylated lysine
Modified residue251UniProtPhosphoserine

Post-translational modification

Ubiquitinated.

Keywords

Proteomic databases

PTM databases

Expression

Gene expression databases

Organism-specific databases

Interaction

Subunit

Component of the SMC5-SMC6 complex which consists at least of SMC5, SMC6, NSMCE2, NSMCE1, NSMCE4A or EID3 and NSMCE3. NSMCE1, NSMCE4A or EID3 and NSMCE3 probably form a subcomplex that bridges the head domains of the SMC5-SMC6 heterodimer (PubMed:18086888, PubMed:20864041).
Interacts with NSMCE3 (PubMed:27427983).
Interacts with MAGEF1 (PubMed:29225034).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntAct
BINARY Q8WV22MAGEF1 Q9HAY22EBI-2557372, EBI-5525855
BINARY Q8WV22NSMCE3 Q96MG722EBI-2557372, EBI-2557356
BINARY Q8WV22SMC6 Q96SB811EBI-2557372, EBI-605415

Complex viewer

View interactors in UniProtKB
View CPX-5992 in Complex Portal

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for region, zinc finger, compositional bias.

Type
IDPosition(s)Description
Region1-102Interaction with NSMCE3
Zinc finger191-232RING-type; atypical
Region246-266Disordered
Compositional bias256-266Basic residues

Sequence similarities

Belongs to the NSE1 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    266
  • Mass (Da)
    30,855
  • Last updated
    2008-10-14 v5
  • MD5 Checksum
    A97D57A64F75C21FE5E67CF01F38086A
MQGSTRRMGVMTDVHRRFLQLLMTHGVLEEWDVKRLQTHCYKVHDRNATVDKLEDFINNINSVLESLYIEIKRGVTEDDGRPIYALVNLATTSISKMATDFAENELDLFRKALELIIDSETGFASSTNILNLVDQLKGKKMRKKEAEQVLQKFVQNKWLIEKEGEFTLHGRAILEMEQYIRETYPDAVKICNICHSLLIQGQSCETCGIRMHLPCVAKYFQSNAEPRCPHCNDYWPHEIPKVFDPEKERESGVLKSNKKSLRSRQH

Computationally mapped potential isoform sequences

There are 6 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
H3BV05H3BV05_HUMANNSMCE178
H3BSL0H3BSL0_HUMANNSMCE1138
H3BR73H3BR73_HUMANNSMCE1144
H3BR31H3BR31_HUMANNSMCE170
H3BN81H3BN81_HUMANNSMCE173
I3L1I3I3L1I3_HUMANNSMCE1193

Sequence caution

The sequence AAF29011.1 differs from that shown. Reason: Frameshift
The sequence AAF29015.1 differs from that shown. Reason: Frameshift

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias256-266Basic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF161451
EMBL· GenBank· DDBJ
AAF29011.1
EMBL· GenBank· DDBJ
mRNA Sequence problems.
AF161455
EMBL· GenBank· DDBJ
AAF29015.1
EMBL· GenBank· DDBJ
mRNA Sequence problems.
AC106739
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
CH471145
EMBL· GenBank· DDBJ
EAW55756.1
EMBL· GenBank· DDBJ
Genomic DNA
CH471145
EMBL· GenBank· DDBJ
EAW55757.1
EMBL· GenBank· DDBJ
Genomic DNA
BC018938
EMBL· GenBank· DDBJ
AAH18938.4
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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