Q8WV22 · NSE1_HUMAN
- ProteinNon-structural maintenance of chromosomes element 1 homolog
- GeneNSMCE1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids266 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
RING-type zinc finger-containing E3 ubiquitin ligase that assembles with melanoma antigen protein (MAGE) to catalyze the direct transfer of ubiquitin from E2 ubiquitin-conjugating enzyme to a specific substrate. Within MAGE-RING ubiquitin ligase complex, MAGE stimulates and specifies ubiquitin ligase activity likely through recruitment and/or stabilization of the E2 ubiquitin-conjugating enzyme at the E3:substrate complex. Involved in maintenance of genome integrity, DNA damage response and DNA repair (PubMed:20864041, PubMed:29225034).
NSMCE3/MAGEG1 and NSMCE1 ubiquitin ligase are components of SMC5-SMC6 complex and may positively regulate homologous recombination-mediated DNA repair (PubMed:18086888).
MAGEF1-NSMCE1 ubiquitin ligase promotes proteasomal degradation of MMS19, a key component of the cytosolic iron-sulfur protein assembly (CIA) machinery. Down-regulation of MMS19 impairs the activity of several DNA repair and metabolism enzymes such as ERCC2/XPD, FANCJ, RTEL1 and POLD1 that require iron-sulfur clusters as cofactors (PubMed:29225034).
NSMCE3/MAGEG1 and NSMCE1 ubiquitin ligase are components of SMC5-SMC6 complex and may positively regulate homologous recombination-mediated DNA repair (PubMed:18086888).
MAGEF1-NSMCE1 ubiquitin ligase promotes proteasomal degradation of MMS19, a key component of the cytosolic iron-sulfur protein assembly (CIA) machinery. Down-regulation of MMS19 impairs the activity of several DNA repair and metabolism enzymes such as ERCC2/XPD, FANCJ, RTEL1 and POLD1 that require iron-sulfur clusters as cofactors (PubMed:29225034).
Catalytic activity
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chromosome, centromeric region | |
Cellular Component | chromosome, telomeric region | |
Cellular Component | intracellular membrane-bounded organelle | |
Cellular Component | mitotic spindle pole | |
Cellular Component | nucleoplasm | |
Cellular Component | nucleus | |
Cellular Component | Smc5-Smc6 complex | |
Molecular Function | metal ion binding | |
Molecular Function | protein dimerization activity | |
Molecular Function | ubiquitin protein ligase activity | |
Molecular Function | ubiquitin-protein transferase activity | |
Biological Process | chromatin looping | |
Biological Process | DNA damage response | |
Biological Process | double-strand break repair via homologous recombination | |
Biological Process | protein sumoylation | |
Biological Process | regulation of telomere maintenance |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNon-structural maintenance of chromosomes element 1 homolog
- EC number
- Short namesNon-SMC element 1 homolog
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ8WV22
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Disease & Variants
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Natural variant | VAR_029822 | 38 | in dbSNP:rs7195194 | ||
Natural variant | VAR_029823 | 47 | in dbSNP:rs17856580 | ||
Variants
![](/variants.8e7f84.jpg)
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The viewer provides 294 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Genetic variation databases
PTM/Processing
Features
Showing features for chain, modified residue (large scale data), modified residue.
Type | ID | Position(s) | Source | Description | ||
---|---|---|---|---|---|---|
Chain | PRO_0000270944 | 1-266 | UniProt | Non-structural maintenance of chromosomes element 1 homolog | ||
Modified residue (large scale data) | 241 | PTMeXchange | Sumoylated lysine | |||
Modified residue | 251 | UniProt | Phosphoserine | |||
Post-translational modification
Ubiquitinated.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Interaction
Subunit
Component of the SMC5-SMC6 complex which consists at least of SMC5, SMC6, NSMCE2, NSMCE1, NSMCE4A or EID3 and NSMCE3. NSMCE1, NSMCE4A or EID3 and NSMCE3 probably form a subcomplex that bridges the head domains of the SMC5-SMC6 heterodimer (PubMed:18086888, PubMed:20864041).
Interacts with NSMCE3 (PubMed:27427983).
Interacts with MAGEF1 (PubMed:29225034).
Interacts with NSMCE3 (PubMed:27427983).
Interacts with MAGEF1 (PubMed:29225034).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | IntAct | |
---|---|---|---|---|---|
BINARY | Q8WV22 | MAGEF1 Q9HAY2 | 2 | EBI-2557372, EBI-5525855 | |
BINARY | Q8WV22 | NSMCE3 Q96MG7 | 22 | EBI-2557372, EBI-2557356 | |
BINARY | Q8WV22 | SMC6 Q96SB8 | 11 | EBI-2557372, EBI-605415 |
Complex viewer
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, zinc finger, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-102 | Interaction with NSMCE3 | |||
Zinc finger | 191-232 | RING-type; atypical | |||
Region | 246-266 | Disordered | |||
Compositional bias | 256-266 | Basic residues | |||
Sequence similarities
Belongs to the NSE1 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length266
- Mass (Da)30,855
- Last updated2008-10-14 v5
- MD5 ChecksumA97D57A64F75C21FE5E67CF01F38086A
Computationally mapped potential isoform sequences
There are 6 potential isoforms mapped to this entry
Sequence caution
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 256-266 | Basic residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF161451 EMBL· GenBank· DDBJ | AAF29011.1 EMBL· GenBank· DDBJ | mRNA | Sequence problems. | |
AF161455 EMBL· GenBank· DDBJ | AAF29015.1 EMBL· GenBank· DDBJ | mRNA | Sequence problems. | |
AC106739 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CH471145 EMBL· GenBank· DDBJ | EAW55756.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CH471145 EMBL· GenBank· DDBJ | EAW55757.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC018938 EMBL· GenBank· DDBJ | AAH18938.4 EMBL· GenBank· DDBJ | mRNA |