Q8TAE8 · G45IP_HUMAN
- ProteinLarge ribosomal subunit protein mL64
- GeneGADD45GIP1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids222 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Acts as a negative regulator of G1 to S cell cycle phase progression by inhibiting cyclin-dependent kinases. Inhibitory effects are additive with GADD45 proteins but occurs also in the absence of GADD45 proteins. Acts as a repressor of the orphan nuclear receptor NR4A1 by inhibiting AB domain-mediated transcriptional activity. May be involved in the hormone-mediated regulation of NR4A1 transcriptional activity. May play a role in mitochondrial protein synthesis.
Miscellaneous
Cells overexpressing GADD45GIP1 were more likely to be in G1 and less likely to be in S phase and grow more slowly than control cells. Inhibiting the expression of GADD45GIP1 promotes cell cycle progression.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | mitochondrial inner membrane | |
Cellular Component | mitochondrial large ribosomal subunit | |
Cellular Component | mitochondrial matrix | |
Cellular Component | mitochondrion | |
Cellular Component | nucleoplasm | |
Biological Process | mitochondrial translation |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameLarge ribosomal subunit protein mL64
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ8TAE8
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Using N-terminally tagged constructs, has been found in the nucleus (PubMed:12482659).
C-terminally tagged constructs are targeted exclusively to mitochondria (PubMed:22453275).
This discrepancy may be explained by masking of a potential N-terminal mitochondrial targeting signal by the tag (PubMed:22453275).
C-terminally tagged constructs are targeted exclusively to mitochondria (PubMed:22453275).
This discrepancy may be explained by masking of a potential N-terminal mitochondrial targeting signal by the tag (PubMed:22453275).
Keywords
- Cellular component
Disease & Variants
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 320 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Genetic variation databases
PTM/Processing
Features
Showing features for chain, modified residue (large scale data).
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Chain | PRO_0000228620 | 1-222 | UniProt | Large ribosomal subunit protein mL64 | |||
Sequence: MAASVRQARSLLGVAATLAPGSRGYRARPPPRRRPGPRWPDPEDLLTPRWQLGPRYAAKQFARYGAASGVVPGSLWPSPEQLRELEAEEREWYPSLATMQESLRVKQLAEEQKRREREQHIAECMAKMPQMIVNWQQQQRENWEKAQADKERRARLQAEAQELLGYQVDPRSARFQELLQDLEKKERKRLKEEKQKRKKEARAAALAAAVAQDPAASGAPSS | |||||||
Modified residue (large scale data) | 166 | PRIDE | Phosphotyrosine | ||||
Sequence: Y | |||||||
Modified residue (large scale data) | 221 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 222 | PRIDE | Phosphoserine | ||||
Sequence: S |
Proteomic databases
PTM databases
Expression
Tissue specificity
Widely expressed. Highly expressed in the thyroid gland, heart, lymph nodes, trachea and adrenal tissues. Expressed at lower level in liver skeletal muscle, kidney, pancreas, testis, ovary and stomach. Barely detectable in adrenal adenoma and papillary thyroid cancer.
Induction
Down-regulated by p53/TP53 in apoptotic cells.
Gene expression databases
Organism-specific databases
Interaction
Subunit
Component of the mitochondrial large ribosomal subunit (mt-LSU) (PubMed:25278503, PubMed:25838379, PubMed:28892042, PubMed:35177605).
Mature mammalian 55S mitochondrial ribosomes consist of a small (28S) and a large (39S) subunit. The 28S small subunit contains a 12S ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial valine transfer RNA (mt-tRNA(Val)), which plays an integral structural role, and 52 different proteins (PubMed:23908630, PubMed:25278503, PubMed:25838379).
Interacts with GADD45A, GADD45B and GADD45G (PubMed:12716909).
Interacts with NR4A1 via the NR4A1 AB domain (PubMed:15459248).
Interacts with ATAD3A and ATAD3B (PubMed:22453275).
Mature mammalian 55S mitochondrial ribosomes consist of a small (28S) and a large (39S) subunit. The 28S small subunit contains a 12S ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial valine transfer RNA (mt-tRNA(Val)), which plays an integral structural role, and 52 different proteins (PubMed:23908630, PubMed:25278503, PubMed:25838379).
Interacts with GADD45A, GADD45B and GADD45G (PubMed:12716909).
Interacts with NR4A1 via the NR4A1 AB domain (PubMed:15459248).
Interacts with ATAD3A and ATAD3B (PubMed:22453275).
(Microbial infection) Interacts with the human papilloma virus type 16 (HPV 16) minor capsid protein L2.
Binary interactions
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, coiled coil, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 19-46 | Disordered | ||||
Sequence: APGSRGYRARPPPRRRPGPRWPDPEDLL | ||||||
Coiled coil | 99-212 | |||||
Sequence: MQESLRVKQLAEEQKRREREQHIAECMAKMPQMIVNWQQQQRENWEKAQADKERRARLQAEAQELLGYQVDPRSARFQELLQDLEKKERKRLKEEKQKRKKEARAAALAAAVAQ | ||||||
Motif | 184-200 | Nuclear localization signal | ||||
Sequence: KKERKRLKEEKQKRKKE | ||||||
Region | 188-222 | Disordered | ||||
Sequence: KRLKEEKQKRKKEARAAALAAAVAQDPAASGAPSS |
Sequence similarities
Belongs to the mitochondrion-specific ribosomal protein mL64 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length222
- Mass (Da)25,384
- Last updated2002-06-01 v1
- Checksum304C397AEC958804
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 7-10 | in Ref. 1; CAD22344 | ||||
Sequence: QARS → KALN |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ429498 EMBL· GenBank· DDBJ | CAD22344.1 EMBL· GenBank· DDBJ | mRNA | ||
AF479749 EMBL· GenBank· DDBJ | AAL85877.1 EMBL· GenBank· DDBJ | mRNA | ||
AF475095 EMBL· GenBank· DDBJ | AAM10639.1 EMBL· GenBank· DDBJ | mRNA | ||
BC004944 EMBL· GenBank· DDBJ | AAH04944.1 EMBL· GenBank· DDBJ | mRNA | ||
BC013039 EMBL· GenBank· DDBJ | AAH13039.2 EMBL· GenBank· DDBJ | mRNA | ||
BC013027 EMBL· GenBank· DDBJ | AAH13027.2 EMBL· GenBank· DDBJ | mRNA | ||
BC069200 EMBL· GenBank· DDBJ | AAH69200.1 EMBL· GenBank· DDBJ | mRNA | ||
AJ295986 EMBL· GenBank· DDBJ | CAC82499.1 EMBL· GenBank· DDBJ | mRNA | ||
AF147080 EMBL· GenBank· DDBJ | - | mRNA | No translation available. |