Q8H0D3 · DXMT2_COFAR

Function

function

Involved in the biosynthesis of caffeine. Catalyzes the conversion of 7-methylxanthine (7mX) to theobromine and of theobromine to caffeine. Has 1-N-methylation activity.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
125.6 μM7-methylxanthine for the recombinant protein
30.8 μMparaxanthine for the recombinant protein
157 μMtheobromine for the recombinant protein

Pathway

Alkaloid biosynthesis.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site18S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site18substrate
Binding site21-25substrate
Binding site60S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site60-61S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site61S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site66S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site98-101S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site139-141S-adenosyl-L-methionine (UniProtKB | ChEBI)
Site154Involved in substrate discrimination
Binding site156-158S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site157-161substrate
Binding site178Mg2+ (UniProtKB | ChEBI)
Binding site237substrate
Binding site260Mg2+ (UniProtKB | ChEBI)
Binding site262Mg2+ (UniProtKB | ChEBI)
Binding site263Mg2+ (UniProtKB | ChEBI)
Site266Involved in substrate discrimination
Site328Involved in substrate discrimination
Binding site333substrate
Site343Involved in substrate discrimination
Binding site368substrate

GO annotations

AspectTerm
Molecular Functionmetal ion binding
Molecular Functionparaxanthine:S-adenosyl-L-methionine 3-N-methyltransferase activity
Molecular FunctionS-adenosylmethionine-dependent methyltransferase activity
Molecular Functiontheobromine:S-adenosyl-L-methionine 1-N-methyltransferase activity
Biological Processalkaloid metabolic process
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    3,7-dimethylxanthine N-methyltransferase 2
  • EC number
  • Short names
    CaDXMT2
  • Alternative names
    • Caffeine synthase 1
    • Caffeine synthase 6
      (CtCS6
      )

Gene names

    • Name
      DXMT2
    • Synonyms
      CCS1
      , CS6

Organism names

  • Taxonomic identifier
  • Strains
    • cv. Caturra
    • cv. ET39
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Gentianales > Rubiaceae > Ixoroideae > Gardenieae complex > Bertiereae - Coffeeae clade > Coffeeae > Coffea

Accessions

  • Primary accession
    Q8H0D3
  • Secondary accessions
    • G9BZJ8

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004083091-3843,7-dimethylxanthine N-methyltransferase 2

Expression

Tissue specificity

Highly expressed in developing endosperm (PubMed:12527364).
Detected in young leaves and flower buds (PubMed:12527364, PubMed:25249475).
Present in immature fruits (grains), but barely in mature fruits (PubMed:25249475).

Structure

Family & Domains

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    384
  • Mass (Da)
    43,276
  • Last updated
    2003-03-01 v1
  • Checksum
    5DC3ED92FD94BEF3
MELQEVLHMNGGEGDTSYAKNSSYNLFLIRVKPVLEQCIQELLRANLPNINKCFKVGDLGCASGPNTFSTVRDIVQSIDKVGQEKKNELERPTIQIFLNDLFQNDFNSVFKLLPSFYRNLEKENGRKIGSCLIGAMPGSFYSRLFPEESMHFLHSCYCLHWLSQVPSGLVTELGISANKGCIYSSKASGPPIKKAYLDQFTKDFTTFLRIHSEELISRGRMLLTFICKEDEFDHPNSMDLLEMSINDLVIEGHLEEEKLDSFNVPIYAPSTEEVKRIVEEEGSFEILYLETFYAPYDAGFSIDDDYQGRSHSPVSCDEHARAAHVASVVRSIYEPILASHFGEAILPDLSHRIAKNAAKVLRSGKGFYDSVIISLAKKPEKADM

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict177in Ref. 2; AEV57593/AIG53793/AIG53794
Sequence conflict189-193in Ref. 2; AEV57593/AIG53793/AIG53794
Sequence conflict250in Ref. 2; AEV57593/AIG53793/AIG53794

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB086414
EMBL· GenBank· DDBJ
BAC43760.1
EMBL· GenBank· DDBJ
mRNA
HQ724310
EMBL· GenBank· DDBJ
AEV57593.1
EMBL· GenBank· DDBJ
Genomic DNA
KJ577792
EMBL· GenBank· DDBJ
AIG53793.1
EMBL· GenBank· DDBJ
Genomic DNA
KJ577793
EMBL· GenBank· DDBJ
AIG53794.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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