Q89B09 · DNAG_BUCBP

Function

function

RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.

Catalytic activity

  • ssDNA + n NTP = ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate.
    EC:2.7.7.101 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per monomer.
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds two Mg2+ per subunit.

Features

Showing features for binding site.

157850100150200250300350400450500550
TypeIDPosition(s)Description
Binding site263Mg2+ 1 (UniProtKB | ChEBI); catalytic
Binding site307Mg2+ 1 (UniProtKB | ChEBI); catalytic
Binding site307Mg2+ 2 (UniProtKB | ChEBI)
Binding site309Mg2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular ComponentDNA-directed RNA polymerase complex
Cellular Componentprimosome complex
Molecular FunctionDNA binding
Molecular FunctionDNA primase activity
Molecular Functionzinc ion binding

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA primase
  • EC number

Gene names

    • Name
      dnaG
    • Ordered locus names
      bbp_053

Organism names

Accessions

  • Primary accession
    Q89B09

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001804831-578DNA primase

Interaction

Subunit

Monomer. Interacts with DnaB.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for zinc finger, domain.

TypeIDPosition(s)Description
Zinc finger40-64CHC2-type
Domain257-339Toprim

Domain

Contains an N-terminal zinc-binding domain, a central core domain that contains the primase activity, and a C-terminal DnaB-binding domain.

Sequence similarities

Belongs to the DnaG primase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    578
  • Mass (Da)
    67,638
  • Last updated
    2003-05-30 v1
  • Checksum
    D948F8BFC183633C
MPSLIPKEFINELIFKTNIIDIINTRLPLKKVGKNYNTHCPFHQEKTPSFTVNFEKQFYFCFGCNTHGNIIDFLMNYEQLTFVESIKELAKIHGLTIPCKNINKEKLFNYEKITNLYLLTKNIADLYHRNIFKTEYAYQYILNRGIDKSMIKYFNLGFSPKNWYDLEKKNKKQCYNQKELQEIGILKTTILGYTYDRFKNRIIFPIRNKNGNIVGFGGRTLNNHIPKYINSPETQIFHKGFQLYGLYEMLKTNPKPKQILIVEGYVDVIALVQFKINYTISTLGTIISNEQIKLLFRTSNTIIFCYDGDVSGQKAAWRTLNISLSHIHDGKNVKFIFLPNNEDPDSIIRKEGHDNFKIRIKKSIDFSKFLLQTLFKKTDLNSISEKSHTSTIAISLIRKIPGKITQTYLFQTLSKKIGILDYHVLINNNINNINTKIYTKKPIKKTTIRIVIALLIQNPWLALTLPSLKHLQNYKIIGLSCFLDLVEKCISMPNSNTGQILEKYRNKNIFKHLANLAKWDHMIHNEKIKDFFLDSLTKICDIILEDRQNKLISQERLNGLNKQEKYELWSINKELAKK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE016826
EMBL· GenBank· DDBJ
AAO26792.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp