Q89B09 · DNAG_BUCBP
- ProteinDNA primase
- GenednaG
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids578 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.
Catalytic activity
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 zinc ion per monomer.
Note: Binds two Mg2+ per subunit.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | DNA-directed RNA polymerase complex | |
Cellular Component | primosome complex | |
Molecular Function | DNA binding | |
Molecular Function | DNA primase activity | |
Molecular Function | zinc ion binding |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA primase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Erwiniaceae > Buchnera
Accessions
- Primary accessionQ89B09
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000180483 | 1-578 | DNA primase | |||
Sequence: MPSLIPKEFINELIFKTNIIDIINTRLPLKKVGKNYNTHCPFHQEKTPSFTVNFEKQFYFCFGCNTHGNIIDFLMNYEQLTFVESIKELAKIHGLTIPCKNINKEKLFNYEKITNLYLLTKNIADLYHRNIFKTEYAYQYILNRGIDKSMIKYFNLGFSPKNWYDLEKKNKKQCYNQKELQEIGILKTTILGYTYDRFKNRIIFPIRNKNGNIVGFGGRTLNNHIPKYINSPETQIFHKGFQLYGLYEMLKTNPKPKQILIVEGYVDVIALVQFKINYTISTLGTIISNEQIKLLFRTSNTIIFCYDGDVSGQKAAWRTLNISLSHIHDGKNVKFIFLPNNEDPDSIIRKEGHDNFKIRIKKSIDFSKFLLQTLFKKTDLNSISEKSHTSTIAISLIRKIPGKITQTYLFQTLSKKIGILDYHVLINNNINNINTKIYTKKPIKKTTIRIVIALLIQNPWLALTLPSLKHLQNYKIIGLSCFLDLVEKCISMPNSNTGQILEKYRNKNIFKHLANLAKWDHMIHNEKIKDFFLDSLTKICDIILEDRQNKLISQERLNGLNKQEKYELWSINKELAKK |
Interaction
Structure
Family & Domains
Features
Showing features for zinc finger, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Zinc finger | 40-64 | CHC2-type | ||||
Sequence: CPFHQEKTPSFTVNFEKQFYFCFGC | ||||||
Domain | 257-339 | Toprim | ||||
Sequence: KQILIVEGYVDVIALVQFKINYTISTLGTIISNEQIKLLFRTSNTIIFCYDGDVSGQKAAWRTLNISLSHIHDGKNVKFIFLP |
Domain
Contains an N-terminal zinc-binding domain, a central core domain that contains the primase activity, and a C-terminal DnaB-binding domain.
Sequence similarities
Belongs to the DnaG primase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length578
- Mass (Da)67,638
- Last updated2003-05-30 v1
- ChecksumD948F8BFC183633C
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE016826 EMBL· GenBank· DDBJ | AAO26792.1 EMBL· GenBank· DDBJ | Genomic DNA |