Q6IV82 · TAZ_PONPY

Function

function

Acyltransferase required to remodel newly synthesized phospholipid cardiolipin (1',3'-bis-[1,2-diacyl-sn-glycero-3-phospho]-glycerol or CL), a key component of the mitochondrial inner membrane, with tissue specific acyl chains necessary for adequate mitochondrial function (By similarity).
Its role in cellular physiology is to improve mitochondrial performance (By similarity).
CL is critical for the coassembly of lipids and proteins in mitochondrial membranes, for instance, remodeling of the acyl groups of CL in the mitochondrial inner membrane affects the assembly and stability of respiratory chain complex IV and its supercomplex forms (By similarity).
Catalyzes the transacylation between phospholipids and lysophospholipids, with the highest rate being between phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) and CL. Catalyzes both 1-acyl-sn-glycero-3-phosphocholine (lysophosphatidylcholine or LPC) reacylation and PC-CL transacylation, that means, it exchanges acyl groups between CL and PC by a combination of forward and reverse transacylations. Also catalyzes transacylations between other phospholipids such as phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine or PE) and CL, between PC and PE, and between PC and phosphatidate (1,2-diacyl-sn-glycero-3-phosphate or PA), although at lower rate. Not regiospecific, it transfers acyl groups into any of the sn-1 and sn-2 positions of the monolysocardiolipin (MLCL), which is an important prerequisite for uniformity and symmetry in CL acyl distribution. Cannot transacylate dilysocardiolipin (DLCL), thus, the role of MLCL is limited to that of an acyl acceptor. CoA-independent, it can reshuffle molecular species within a single phospholipid class. Redistributes fatty acids between MLCL, CL, and other lipids, which prolongs the half-life of CL. Its action is completely reversible, which allows for cyclic changes, such as fission and fusion or bending and flattening of the membrane. Hence, by contributing to the flexibility of the lipid composition, it plays an important role in the dynamics of mitochondria membranes. Essential for the final stage of spermatogenesis, spermatid individualization (By similarity).
Required for the initiation of mitophagy (By similarity).
Required to ensure progression of spermatocytes through meiosis (By similarity).

Miscellaneous

The enzyme was named after a masochistic character Tafazzi, once popular on Italian television, apparently due to the difficulty encountered for its identification and characterization.

Catalytic activity

  • a 1-acyl-sn-glycero-3-phosphate + a 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) = 1-acyl-sn-glycero-3-phospho-(1'-sn-glycerol) + a 1,2-diacyl-sn-glycero-3-phosphate
    This reaction proceeds in the forward
    and the backward
    directions.
  • 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate = 1-(9Z)-octadecenoyl-2-(9Z,12Z)-octadecadienoyl-sn-glycero-3-phosphate + 1-hexadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol)
    This reaction proceeds in the forward
    and the backward
    directions.
  • 1'-[1,2-diacyl-sn-glycero-3-phospho],3'-[1-acyl-sn-glycero-3-phospho]-glycerol + a 1,2-diacyl-sn-glycero-3-phosphocholine = a cardiolipin + a 1-acyl-sn-glycero-3-phosphocholine
    This reaction proceeds in the forward
    and the backward
    directions.
  • 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + 1-hexadecanoyl-sn-glycero-3-phosphocholine = 2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine
    This reaction proceeds in the forward
    and the backward
    directions.
  • 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-hexadecanoyl-sn-glycero-3-phosphocholine = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine
    This reaction proceeds in the forward
    and the backward
    directions.

Pathway

Phospholipid metabolism.

GO annotations

AspectTerm
Cellular Componentmitochondrial inner membrane
Cellular Componentmitochondrial outer membrane
Cellular Componentmitochondrion
Molecular Function1-acylglycerophosphocholine O-acyltransferase activity
Biological Processcardiolipin acyl-chain remodeling
Biological Processcardiolipin metabolic process
Biological Processheart morphogenesis
Biological Processinner mitochondrial membrane organization
Biological Processmitochondrion organization
Biological Processmitophagy
Biological Processspermatocyte division

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Tafazzin
  • EC number
  • Short names
    Taz

Gene names

    • Name
      TAFAZZIN
    • Synonyms
      TAZ

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Pongo

Accessions

  • Primary accession
    Q6IV82

Subcellular Location

Mitochondrion outer membrane
; Peripheral membrane protein
Mitochondrion inner membrane
; Peripheral membrane protein

Features

Showing features for topological domain, intramembrane.

TypeIDPosition(s)Description
Topological domain1-14Mitochondrial intermembrane
Intramembrane15-35
Topological domain36-262Mitochondrial intermembrane

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002209311-262Tafazzin

Interaction

Subunit

Associates with multiple protein complexes.

Structure

3D structure databases

Family & Domains

Features

Showing features for motif, region.

TypeIDPosition(s)Description
Motif69-74HXXXXD motif
Region82-92Mitochondrial targeting sequence
Region155-190Mitochondrial targeting sequence

Domain

The HXXXXD motif is essential for acyltransferase activity.

Sequence similarities

Belongs to the taffazin family.

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

Q6IV82-2

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    262
  • Mass (Da)
    30,208
  • Last updated
    2022-02-23 v2
  • Checksum
    C1B4E4BDEDC16AAB
MPLHVKWPFPAVPPLTWTLASSVVMGLVGTYSCFWTKYMNHLTVHNKEVLYELIENRGPATPLITVSNHQSCMDDPHLWGILKLRHIWNLKLMRWTPAAADICFTKELHSHFFSLGKCVPVCRGDGVYQKGMDFILEKLNHGDWVHIFPEGKVNMSSEFLRFKWGIGRLIAECHLNPIILPLWHVGMNDVLPNSPPYFPRFGQKITVLIGKPFSALPVLERLRAENKSAVEMRKVLTDFIQEEFQRLKTQAEQLHNHLQPGR

Q6IV82-1

  • Name
    1
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 123-123: R → RGAEFFQPENEGKGVLDTGRHMPGAGKRREK

Features

Showing features for alternative sequence.

TypeIDPosition(s)Description
Alternative sequenceVSP_061373123in isoform 1

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY621046
EMBL· GenBank· DDBJ
AAT45906.1
EMBL· GenBank· DDBJ
Genomic DNA
AY621044
EMBL· GenBank· DDBJ
AAT45906.1
EMBL· GenBank· DDBJ
Genomic DNA
AY621045
EMBL· GenBank· DDBJ
AAT45906.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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