Q6DF80 · INSI1_XENLA
- ProteinInsulin-induced gene 1 protein
- Geneinsig1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids251 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score2/5
Function
function
Oxysterol-binding protein that mediates feedback control of cholesterol synthesis by controlling both endoplasmic reticulum to Golgi transport of scap and degradation of hmgcr. Acts as a negative regulator of cholesterol biosynthesis by mediating the retention of the SCAP-SREBP complex in the endoplasmic reticulum, thereby blocking the processing of sterol regulatory element-binding proteins (SREBPs). Binds oxysterol, including 25-hydroxycholesterol, regulating interaction with scap and retention of the SCAP-SREBP complex in the endoplasmic reticulum. In presence of oxysterol, interacts with scap, retaining the SCAP-SREBP complex in the endoplasmic reticulum, thereby preventing scap from escorting SREBPs to the Golgi. Sterol deprivation reduces oxysterol-binding, disrupting the interaction between insig1 and scap, thereby promoting Golgi transport of the SCAP-SREBP complex, followed by processing and nuclear translocation of SREBPs. Also regulates cholesterol synthesis by regulating degradation of hmgcr.
Features
Showing features for site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 145 | Required for the recognition of 25-hydroxycholesterol | ||||
Sequence: F |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Molecular Function | oxysterol binding | |
Biological Process | cholesterol metabolic process |
Keywords
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameInsulin-induced gene 1 protein
- Short namesINSIG-1
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Amphibia > Batrachia > Anura > Pipoidea > Pipidae > Xenopodinae > Xenopus > Xenopus
Accessions
- Primary accessionQ6DF80
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-58 | Cytoplasmic | ||||
Sequence: MQTLEEHCWSCSCTRGRDKKGTKVSAWLARRVGKAMSSLNSLLSLAYSTLASSEGRSL | ||||||
Transmembrane | 59-81 | Helical; Name=1 | ||||
Sequence: IQRSLVLFTVGVFLALVLNLLQI | ||||||
Topological domain | 82-100 | Extracellular | ||||
Sequence: QRNVTLFPEEVIATIFSSA | ||||||
Transmembrane | 101-118 | Helical; Name=2 | ||||
Sequence: WWVPPCCGTAAAVVGLLY | ||||||
Topological domain | 119-133 | Cytoplasmic | ||||
Sequence: PCIDSRIGEPHKFKR | ||||||
Transmembrane | 134-156 | Helical; Name=3 | ||||
Sequence: EWASVMRCIAVFVGINHASAKLD | ||||||
Topological domain | 157-159 | Extracellular | ||||
Sequence: FAN | ||||||
Transmembrane | 160-178 | Helical; Name=4 | ||||
Sequence: NVQLSLTLAALSLGLWWTF | ||||||
Topological domain | 179-183 | Cytoplasmic | ||||
Sequence: DRSRS | ||||||
Transmembrane | 184-205 | Helical; Name=5 | ||||
Sequence: GLGLGITIAFLATLITQFLVYN | ||||||
Topological domain | 206-219 | Extracellular | ||||
Sequence: GVYQYTSPDFLYIR | ||||||
Transmembrane | 220-237 | Helical; Name=6 | ||||
Sequence: SWLPCIFFSGGVTVGNIG | ||||||
Topological domain | 238-251 | Cytoplasmic | ||||
Sequence: RQLAMGSSEKTHGD |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000287390 | 1-251 | Insulin-induced gene 1 protein | |||
Sequence: MQTLEEHCWSCSCTRGRDKKGTKVSAWLARRVGKAMSSLNSLLSLAYSTLASSEGRSLIQRSLVLFTVGVFLALVLNLLQIQRNVTLFPEEVIATIFSSAWWVPPCCGTAAAVVGLLYPCIDSRIGEPHKFKREWASVMRCIAVFVGINHASAKLDFANNVQLSLTLAALSLGLWWTFDRSRSGLGLGITIAFLATLITQFLVYNGVYQYTSPDFLYIRSWLPCIFFSGGVTVGNIGRQLAMGSSEKTHGD |
Expression
Gene expression databases
Interaction
Subunit
Interacts with scap; interaction is direct and only takes place in the presence of sterols; it prevents interaction between scap and the coat protein complex II (COPII). Associates with the SCAP-SREBP complex; association is mediated via its interaction with scap and only takes place in the presence of sterols.
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 245-251 | KxHxx | ||||
Sequence: SEKTHGD |
Domain
The KxHxx motif mediates association with the coatomer complex.
Binds oxysterols in a pocket within their transmembrane domains and interacts with SCAP via transmembrane domains 3 and 4.
Sequence similarities
Belongs to the INSIG family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length251
- Mass (Da)27,569
- Last updated2004-08-16 v1
- ChecksumC4A3B397D33BAD88
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BC076862 EMBL· GenBank· DDBJ | AAH76862.1 EMBL· GenBank· DDBJ | mRNA |