Q5ZYK6 · DAPF_LEGPH
- ProteinDiaminopimelate epimerase
- GenedapF
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids277 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.
Catalytic activity
- (2S,6S)-2,6-diaminoheptanedioate = meso-2,6-diaminoheptanedioate
Pathway
Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1.
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 13 | substrate | ||||
Sequence: N | ||||||
Binding site | 46 | substrate | ||||
Sequence: Q | ||||||
Binding site | 66 | substrate | ||||
Sequence: N | ||||||
Active site | 75 | Proton donor | ||||
Sequence: C | ||||||
Binding site | 76-77 | substrate | ||||
Sequence: GN | ||||||
Binding site | 160 | substrate | ||||
Sequence: N | ||||||
Site | 162 | Could be important to modulate the pK values of the two catalytic cysteine residues | ||||
Sequence: H | ||||||
Binding site | 193 | substrate | ||||
Sequence: N | ||||||
Site | 211 | Could be important to modulate the pK values of the two catalytic cysteine residues | ||||
Sequence: E | ||||||
Binding site | 211-212 | substrate | ||||
Sequence: ER | ||||||
Active site | 220 | Proton acceptor | ||||
Sequence: C | ||||||
Binding site | 221-222 | substrate | ||||
Sequence: GS | ||||||
Site | 271 | Important for dimerization | ||||
Sequence: Y |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | diaminopimelate epimerase activity | |
Biological Process | lysine biosynthetic process via diaminopimelate |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDiaminopimelate epimerase
- EC number
- Short namesDAP epimerase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Legionellales > Legionellaceae > Legionella
Accessions
- Primary accessionQ5ZYK6
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000011895 | 1-277 | Diaminopimelate epimerase | |||
Sequence: MGIKFTKMHGLGNDFIVLDGVNQSIQLTVEQIQKLANRHTGIGFDQCLLIESSQTEGIDFNYRIFNADGQEVGQCGNGARCIALFARYYGLTAKNKLTVATKTTLMDLIINEDNSVSVNMGVPRLAPGEIPLLADRQSPEYSLELNNGNTVNLHAISVGNPHAVLLVENIDTAPVNSLGQQISFHPQFPEQVNVGFMQIINHEKINLRVYERGCGETIACGSGAVAAAAIARLFYNLSDKITVHLPGGDLCIQWPCPTAPIILTGPAAFVYEGTLLS |
Proteomic databases
Interaction
Structure
Sequence
- Sequence statusComplete
- Length277
- Mass (Da)29,953
- Last updated2004-11-23 v1
- ChecksumDE73BE862397C9A7
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE017354 EMBL· GenBank· DDBJ | AAU26463.1 EMBL· GenBank· DDBJ | Genomic DNA |