Q5U924 · HADB_CLODI

Function

function

Involved in the reductive branch of L-leucine fermentation. Catalyzes the irreversible beta/alpha-elimination of water from (R)-2-hydroxyisocaproyl-CoA to yield isocaprenoyl-CoA. This beta/alpha-dehydration depends on the reductive formation of ketyl radicals on the substrate generated by injection of a single electron from the ATP-dependent activator protein HadI. The enzyme is specific for the R-isomer.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster.

Activity regulation

Activated by HadI.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.05 mM(R)-2-hydroxyisocaproyl-CoA

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site55substrate
Binding site84[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site117[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site346[4Fe-4S] cluster (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionhydro-lyase activity
Molecular Functionmetal ion binding
Biological ProcessL-leucine metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    (R)-2-hydroxyisocaproyl-CoA dehydratase alpha subunit
  • EC number

Gene names

    • Name
      hadB
    • ORF names
      CD630DERM_03970, ERS445050_03310

Encoded on

  • Plasmid 2

Organism names

Accessions

  • Primary accession
    Q5U924

PTM/Processing

Features

Showing features for initiator methionine, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00004356622-408(R)-2-hydroxyisocaproyl-CoA dehydratase alpha subunit

Interaction

Subunit

Part of the heterodimeric complex HadBC composed of (R)-2-hydroxyisocaproyl-CoA dehydratase alpha (HadB) and beta (HadC) subunit.

Family & Domains

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    408
  • Mass (Da)
    46,334
  • Last updated
    2004-12-07 v1
  • Checksum
    702A372CB6796AB5
MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELAEVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEALDMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQFEEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYMAVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMKTLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGKCDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQGLVEVMEERKKLNRGEI

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY772816
EMBL· GenBank· DDBJ
AAV40819.1
EMBL· GenBank· DDBJ
Genomic DNA
LN614756
EMBL· GenBank· DDBJ
CEJ96915.1
EMBL· GenBank· DDBJ
Genomic DNA
LN831031
EMBL· GenBank· DDBJ
CKH72398.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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