Q5U924 · HADB_CLODI
- Protein(R)-2-hydroxyisocaproyl-CoA dehydratase alpha subunit
- GenehadB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids408 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in the reductive branch of L-leucine fermentation. Catalyzes the irreversible beta/alpha-elimination of water from (R)-2-hydroxyisocaproyl-CoA to yield isocaprenoyl-CoA. This beta/alpha-dehydration depends on the reductive formation of ketyl radicals on the substrate generated by injection of a single electron from the ATP-dependent activator protein HadI. The enzyme is specific for the R-isomer.
Catalytic activity
- (R)-2-hydroxy-4-methylpentanoyl-CoA = 4-methylpent-2-enoyl-CoA + H2O
Cofactor
Note: Binds 1 [4Fe-4S] cluster.
Activity regulation
Activated by HadI.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.05 mM | (R)-2-hydroxyisocaproyl-CoA |
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | hydro-lyase activity | |
Molecular Function | metal ion binding | |
Biological Process | L-leucine metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name(R)-2-hydroxyisocaproyl-CoA dehydratase alpha subunit
- EC number
Gene names
Encoded on
- Plasmid 2
Organism names
- Strains
- Taxonomic lineageBacteria > Bacillota > Clostridia > Peptostreptococcales > Peptostreptococcaceae > Clostridioides
Accessions
- Primary accessionQ5U924
PTM/Processing
Features
Showing features for initiator methionine, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Chain | PRO_0000435662 | 2-408 | (R)-2-hydroxyisocaproyl-CoA dehydratase alpha subunit | |||
Sequence: SEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELAEVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEALDMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQFEEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYMAVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMKTLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGKCDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQGLVEVMEERKKLNRGEI |
Interaction
Subunit
Part of the heterodimeric complex HadBC composed of (R)-2-hydroxyisocaproyl-CoA dehydratase alpha (HadB) and beta (HadC) subunit.
Structure
Sequence
- Sequence statusComplete
- Length408
- Mass (Da)46,334
- Last updated2004-12-07 v1
- Checksum702A372CB6796AB5
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY772816 EMBL· GenBank· DDBJ | AAV40819.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LN614756 EMBL· GenBank· DDBJ | CEJ96915.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LN831031 EMBL· GenBank· DDBJ | CKH72398.1 EMBL· GenBank· DDBJ | Genomic DNA |