Q5RBN0 · MID51_PONAB
- ProteinMitochondrial dynamics protein MID51
- GeneMIEF1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids463 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
Mitochondrial outer membrane protein which regulates mitochondrial fission/fusion dynamics. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins. Regulates DNM1L GTPase activity and DNM1L oligomerization. Binds ADP and can also bind GDP, although with lower affinity. Does not bind CDP, UDP, ATP, AMP or GTP. Inhibits DNM1L GTPase activity in the absence of bound ADP. Requires ADP to stimulate DNM1L GTPase activity and the assembly of DNM1L into long, oligomeric tubules with a spiral pattern, as opposed to the ring-like DNM1L oligomers observed in the absence of bound ADP. Does not require ADP for its function in recruiting DNM1L.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 187 | ADP (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 189 | ADP (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 201 | ADP (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 340 | ADP (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 342 | ADP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 368 | ADP (UniProtKB | ChEBI) | ||||
Sequence: K |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrial outer membrane | |
Cellular Component | mitochondrion | |
Molecular Function | nucleotide binding | |
Biological Process | mitochondrial fission | |
Biological Process | positive regulation of mitochondrial fission | |
Biological Process | positive regulation of mitochondrial fusion | |
Biological Process | positive regulation of protein targeting to membrane |
Keywords
- Ligand
Names & Taxonomy
Protein names
- Recommended nameMitochondrial dynamics protein MID51
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Pongo
Accessions
- Primary accessionQ5RBN0
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Mitochondrion outer membrane ; Single-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-23 | Mitochondrial intermembrane | ||||
Sequence: MAGAGERKGKKDDNGIGTAIDFV | ||||||
Transmembrane | 24-46 | Helical | ||||
Sequence: LSNARLVLGVGGAAMLGIATLAV | ||||||
Topological domain | 47-463 | Cytoplasmic | ||||
Sequence: KRMYDRAISAPTSPTRLSHSGKRSWEEPNWMGSPQLLNRDMKTGLSRSLQTLPTDSSAFDTDTFCPPRPKPVARKGQVDLKKSRLRMSLQEKLLPYYRNRAAIPAGEQARAKQAAVDICAELRSFLRAKLPDMPLRDVYLSGSLYDDLQVVTADHIQLIVPLVLEQNLWSCIPGEDTIMNVPGFFLVRRENPEYFPRGSSYWDRCVVGGYLSPKTVADTFEKVVAGSINWPAIGSLLDYVIRPAPPPEALTLEVQYERDKHLFIDFLPSVTLGDTVLVAKPHRLAQYDNLWRLSLRPAETARLRALDQADSGCRSLCLKILKAICKSTPALGHLTASQLTNVILHLAQEEADWSPDMLADRFLQALRGLISYLEAGVLPSALNPKVNLFAELTPEEIDELGYTLYCSLSEPEVLLQT |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000310450 | 1-463 | Mitochondrial dynamics protein MID51 | |||
Sequence: MAGAGERKGKKDDNGIGTAIDFVLSNARLVLGVGGAAMLGIATLAVKRMYDRAISAPTSPTRLSHSGKRSWEEPNWMGSPQLLNRDMKTGLSRSLQTLPTDSSAFDTDTFCPPRPKPVARKGQVDLKKSRLRMSLQEKLLPYYRNRAAIPAGEQARAKQAAVDICAELRSFLRAKLPDMPLRDVYLSGSLYDDLQVVTADHIQLIVPLVLEQNLWSCIPGEDTIMNVPGFFLVRRENPEYFPRGSSYWDRCVVGGYLSPKTVADTFEKVVAGSINWPAIGSLLDYVIRPAPPPEALTLEVQYERDKHLFIDFLPSVTLGDTVLVAKPHRLAQYDNLWRLSLRPAETARLRALDQADSGCRSLCLKILKAICKSTPALGHLTASQLTNVILHLAQEEADWSPDMLADRFLQALRGLISYLEAGVLPSALNPKVNLFAELTPEEIDELGYTLYCSLSEPEVLLQT | ||||||
Modified residue | 55 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 59 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 79 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 94 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Interaction
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 49-195 | Dimerization | ||||
Sequence: MYDRAISAPTSPTRLSHSGKRSWEEPNWMGSPQLLNRDMKTGLSRSLQTLPTDSSAFDTDTFCPPRPKPVARKGQVDLKKSRLRMSLQEKLLPYYRNRAAIPAGEQARAKQAAVDICAELRSFLRAKLPDMPLRDVYLSGSLYDDLQ | ||||||
Region | 57-79 | Disordered | ||||
Sequence: PTSPTRLSHSGKRSWEEPNWMGS | ||||||
Region | 104-123 | Disordered | ||||
Sequence: AFDTDTFCPPRPKPVARKGQ | ||||||
Region | 160-169 | Important for interaction with DNM1L | ||||
Sequence: AAVDICAELR | ||||||
Region | 234-243 | Important for interaction with DNM1L | ||||
Sequence: RRENPEYFPR |
Sequence similarities
Belongs to the MID49/MID51 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length463
- Mass (Da)51,199
- Last updated2004-12-21 v1
- ChecksumCAFEBA4D12715D93
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CR858608 EMBL· GenBank· DDBJ | CAH90830.1 EMBL· GenBank· DDBJ | mRNA |