Q56577 · NHAB_VIBAL
- ProteinNa(+)/H(+) antiporter NhaB
- GenenhaB
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids528 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Na+/H+ antiporter that extrudes sodium in exchange for external protons.
Catalytic activity
- 3 H+(out) + 2 Na+(in) = 3 H+(in) + 2 Na+(out)This reaction proceeds in the forward direction.
Features
Showing features for site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 147 | Important for antiport activity | ||||
Sequence: D |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | sodium:proton antiporter activity |
Keywords
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameNa(+)/H(+) antiporter NhaB
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Vibrionales > Vibrionaceae > Vibrio
Accessions
- Primary accessionQ56577
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-23 | Cytoplasmic | ||||
Sequence: MPISLGNAFIKNFLGKAPDWYKV | ||||||
Transmembrane | 24-46 | Helical | ||||
Sequence: AIIAFLIINPIVFFLINPFVAGW | ||||||
Topological domain | 47-95 | Periplasmic | ||||
Sequence: LLVAEFIFTLAMALKCYPLQPGGLLAIEAIAIGMTSPAQVKHELVANIE | ||||||
Transmembrane | 96-118 | Helical | ||||
Sequence: VLLLLVFMVAGIYFMKHLLLFIF | ||||||
Topological domain | 119-129 | Cytoplasmic | ||||
Sequence: TKILLGIRSKT | ||||||
Transmembrane | 130-163 | Helical | ||||
Sequence: LLSLAFCFAAAFLSAFLDALTVIAVVISVAIGFY | ||||||
Topological domain | 164-239 | Periplasmic | ||||
Sequence: SIYHKVASGNPIGDHDHTQDDTITELTRDDLENYRAFLRSLLMHAGVGTALGGVTTMVGEPQNLIIADQAGWLFGE | ||||||
Transmembrane | 240-262 | Helical | ||||
Sequence: FLIRMSPVTLPVFFCGLITCALV | ||||||
Topological domain | 263-297 | Cytoplasmic | ||||
Sequence: EKLKVFGYGAKLPNNVRQILVDFDNEERKTRTNQD | ||||||
Transmembrane | 298-317 | Helical | ||||
Sequence: VAKLWVQGLIAVWLIVALAL | ||||||
Topological domain | 318-320 | Periplasmic | ||||
Sequence: HLA | ||||||
Transmembrane | 321-340 | Helical | ||||
Sequence: AVGLIGLSVIILATAFTGVI | ||||||
Topological domain | 341-352 | Cytoplasmic | ||||
Sequence: EEHSMGKAFEEA | ||||||
Transmembrane | 353-375 | Helical | ||||
Sequence: LPFTALLAVFFSIVAVIIDQELF | ||||||
Topological domain | 376-389 | Periplasmic | ||||
Sequence: KPVIDAVLAVEDKG | ||||||
Transmembrane | 390-412 | Helical | ||||
Sequence: TQLALFYVANGLLSMVSDNVFVG | ||||||
Topological domain | 413-477 | Cytoplasmic | ||||
Sequence: TVYINEVKTALIEGLITREQFDLLAVAINTGTNLPSVATPNGQAAFLFLLTSALAPLIRLSYGRM | ||||||
Transmembrane | 478-500 | Helical | ||||
Sequence: VIMALPYTIVLAIVGLMGIMFFL | ||||||
Topological domain | 501-528 | Periplasmic | ||||
Sequence: EPATASFYDAGWILPHSGDLTPVVSGGH |
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 147 | Loss of antiport activity at pH 8.5. No change in activity at pH 7.0. | ||||
Sequence: D → E | ||||||
Mutagenesis | 147 | Loss of antiport activity, both at pH 8.5 and at pH 7.0. | ||||
Sequence: D → G, M, or T |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000333144 | 1-528 | Na+/H+ antiporter NhaB | |||
Sequence: MPISLGNAFIKNFLGKAPDWYKVAIIAFLIINPIVFFLINPFVAGWLLVAEFIFTLAMALKCYPLQPGGLLAIEAIAIGMTSPAQVKHELVANIEVLLLLVFMVAGIYFMKHLLLFIFTKILLGIRSKTLLSLAFCFAAAFLSAFLDALTVIAVVISVAIGFYSIYHKVASGNPIGDHDHTQDDTITELTRDDLENYRAFLRSLLMHAGVGTALGGVTTMVGEPQNLIIADQAGWLFGEFLIRMSPVTLPVFFCGLITCALVEKLKVFGYGAKLPNNVRQILVDFDNEERKTRTNQDVAKLWVQGLIAVWLIVALALHLAAVGLIGLSVIILATAFTGVIEEHSMGKAFEEALPFTALLAVFFSIVAVIIDQELFKPVIDAVLAVEDKGTQLALFYVANGLLSMVSDNVFVGTVYINEVKTALIEGLITREQFDLLAVAINTGTNLPSVATPNGQAAFLFLLTSALAPLIRLSYGRMVIMALPYTIVLAIVGLMGIMFFLEPATASFYDAGWILPHSGDLTPVVSGGH |
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length528
- Mass (Da)57,215
- Last updated1996-11-01 v1
- Checksum469F007E38688C6E