Q4WED7 · XANE_ASPFU
- ProteinO-methyltransferase xanE
- GenexanE
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids431 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
O-methyltransferase; part of the gene cluster that mediates the biosynthesis of the isocyanide xanthocillin and its derivatives (PubMed:29844112).
The first step of the pathway consists in the conversion of tyrosine into a vinyl-isonitrile intermediate by the isocyanide synthase xanB (PubMed:29844112).
Subsequent oxidative dimerization of this intermediate to form xanthocillin may involve the cytochrome P450 monooxygenase xanG, whose expression is coregulated with that of XanB (PubMed:29844112).
Xanthocillin can be further modified by the isonitrile hydratase-like protein xanA which introduces N-formyl groups and the methyltransferase xanE which introduces methyl groups, leading to the production of several derivatives including fumiformamide (PubMed:29844112).
Finally, fumiformamide can be subject to both oxidative and reductive cyclization to yield melanocins E and F, respectively (PubMed:29844112).
The first step of the pathway consists in the conversion of tyrosine into a vinyl-isonitrile intermediate by the isocyanide synthase xanB (PubMed:29844112).
Subsequent oxidative dimerization of this intermediate to form xanthocillin may involve the cytochrome P450 monooxygenase xanG, whose expression is coregulated with that of XanB (PubMed:29844112).
Xanthocillin can be further modified by the isonitrile hydratase-like protein xanA which introduces N-formyl groups and the methyltransferase xanE which introduces methyl groups, leading to the production of several derivatives including fumiformamide (PubMed:29844112).
Finally, fumiformamide can be subject to both oxidative and reductive cyclization to yield melanocins E and F, respectively (PubMed:29844112).
Pathway
Secondary metabolite biosynthesis.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | O-methyltransferase activity | |
Biological Process | methylation | |
Biological Process | secondary metabolite biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameO-methyltransferase xanE
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Aspergillus > Aspergillus subgen. Fumigati
Accessions
- Primary accessionQ4WED7
Proteomes
Organism-specific databases
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000445297 | 1-431 | O-methyltransferase xanE | |||
Sequence: MSTQHGPKSTRTNDLYELAVNISSTVEAFVGRLDAIGAERPNLDNPFPEIIQDEGAQLARLKILRLCERLMALVQGPVQWLMFQNMAFLDAACVGAILDMGIHDIIAPGPEPTSLDQIVEATGASKDILKRIMRVCTQRLVFDEIAPEQFIHNGVSLQFLAPPVQALISHACDDGLRMAAHFTDSLKKTDWKGSDDPENTAFSLAFNTNKGLFDYFYTEDLARGQRFALGMAGSEIMKTLTEDMFPFESLPQGAKVVDVGGGRGHVSVRIAEKVPGLNFVVQDDESMLEAGQAEGVPKAVKDRIEFMPHDFFNEQPVKGADVYLLRFILHDHSDSRCAKILSNIVDAMEPGKSRILIDDAIVPSFLGPESSRFFNLLDLYMLAGLNGKERTLEQWNYLIQMVSPKLVLEKVWKTPNGGPESGTILELRLQE |
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length431
- Mass (Da)47,750
- Last updated2005-07-05 v1
- Checksum1A8602775A218751
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AAHF01000011 EMBL· GenBank· DDBJ | EAL86040.1 EMBL· GenBank· DDBJ | Genomic DNA |