Q4QEY9 · TRM5_LEIMA

  • Protein
    tRNA (guanine(37)-N(1))-methyltransferase
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.

Catalytic activity

Features

Showing features for binding site.

168650100150200250300350400450500550600650
TypeIDPosition(s)Description
Binding site428S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site466-467S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site495-496S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site530S-adenosyl-L-methionine (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Cellular Componentmitochondrial matrix
Cellular Componentnucleus
Molecular FunctiontRNA (guanine(37)-N1)-methyltransferase activity
Molecular FunctiontRNA methyltransferase activity
Biological ProcesstRNA N1-guanine methylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA (guanine(37)-N(1))-methyltransferase
  • EC number
  • Alternative names
    • M1G-methyltransferase
    • tRNA [GM37] methyltransferase
    • tRNA methyltransferase 5 homolog

Gene names

    • ORF names
      LMJF_16_0310

Organism names

  • Taxonomic identifier
  • Strain
    • MHOM/IL/81/Friedlin
  • Taxonomic lineage
    Eukaryota > Discoba > Euglenozoa > Kinetoplastea > Metakinetoplastina > Trypanosomatida > Trypanosomatidae > Leishmaniinae > Leishmania

Accessions

  • Primary accession
    Q4QEY9

Proteomes

Organism-specific databases

Subcellular Location

Mitochondrion matrix
Nucleus
Cytoplasm
Note: Predominantly in the mitochondria and in the nucleus.

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004141501-686tRNA (guanine37-N1)-methyltransferase

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region206-244Disordered

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    686
  • Mass (Da)
    73,577
  • Last updated
    2005-07-19 v1
  • Checksum
    FC2908ACFDA3D1F8
MSAKQDGSTAESRKAPHSYRERVNSTVELAALVYRPLTASGALLSILRGKLYRRRSVRNVLDVVAVRSISGGQGHSAESKVRVEYLTPAEGNANGNSISNISAAPCADIRGSSDTAATSSSPSTPAQPTLLEGRCKMCLLDPTVLKATELCPDPASAAQHCTPVFLLGAAVVGSHVTRQLEAAVQAGQLSRKAADLLQQLHERLAGGPSSVSLTEDTDGSEQPQGLPRAAAAPPPPSNKRRASYTGAVEVTFTPRAVELNYQAYTMSELLSMVLPLREHADLVALSGFEQVGHIAHVNLSAAHLPYADIIGQVILDCNETVSVVVNKVDAISSVFREFKMDIIGLRRRTDSVDGNVVAGADLDDAGEAGGSLTAAERQAIALEALSPTYSLAEARVHRLLTATVRQHGCSFRVPYNRVYWNSRLSFEHTRLVDRMRPGDVLFDVMAGVGPFAVPAAKKGVQVFANDLNPVAAQYMKVNAELNHLPANALHVFNMDGRDFLNSVLFTSVTRAADASLPGHLCTGRRHVTMNLPAIAVEFLDVFQPLSSTCAPASEQRCNASAAAVVNERWNHLPAHVDPNAIDRRTLFHVYCFSAAEDLITDAVRQVEVNLGYTLPPENIEETLMVRDVAPTKRMMCVSFTLPPAFWKNLLASRPGQGGEPGGAHAETASTLVEVEQAAKKARPDKM

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FR796412
EMBL· GenBank· DDBJ
CAJ03475.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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