Q4DCH3 · JBP2_TRYCC

Function

function

Dioxygenase that catalyzes the first step of DNA base J (beta-d-glucosyl-HOMedU) biosynthesis by converting thymine to 5-hydroxymethyluracil (HOMedU). DNA base J is a hypermodified thymidine residue found in the genome of kinetoplastid parasites, which is localized primarily to repetitive DNA, namely the telomeres, and is implicated in the regulation of antigenic variation. Probably also acts as a DNA helicase. Recognizes and binds specific regions of the genome, hydrolyzes ATP and allows the DNA base J de novo synthesis. Involved in initial synthesis of DNA base J, JBP1 being able to act via the basal level of DNA base J and propagate further synthesis. In contrast to JBP1, it does not specifically bind DNA base J, however it binds chromatin (By similarity).

Catalytic activity

Cofactor

Fe2+ (UniProtKB | Rhea| CHEBI:29033 )

Note: Binds 1 Fe2+ ion per subunit.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site393Fe cation (UniProtKB | ChEBI); catalytic; for thymine dioxygenase activity
Binding site395Fe cation (UniProtKB | ChEBI); catalytic; for thymine dioxygenase activity
Binding site443Fe cation (UniProtKB | ChEBI); catalytic; for thymine dioxygenase activity
Binding site4572-oxoglutarate (UniProtKB | ChEBI)
Binding site546-553ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentnucleus
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular FunctionATP-dependent chromatin remodeler activity
Molecular FunctionDNA binding
Molecular Functionhelicase activity
Molecular Functionmetal ion binding
Molecular Functionthymine dioxygenase activity
Biological Processbase J metabolic process

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Bifunctional helicase and thymine dioxygenase JBP2
  • Alternative names
    • J-binding protein 2

Including 2 domains:

  • Recommended name
    Probable DNA helicase JBP2
  • EC number
  • Recommended name
    Thymine dioxygenase JBP2
  • EC number

Gene names

    • Name
      JBP2
    • ORF names
      Tc00.1047053508859.74

Organism names

  • Taxonomic identifier
  • Strain
    • CL Brener
  • Taxonomic lineage
    Eukaryota > Discoba > Euglenozoa > Kinetoplastea > Metakinetoplastina > Trypanosomatida > Trypanosomatidae > Trypanosoma > Schizotrypanum

Accessions

  • Primary accession
    Q4DCH3

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003775631-1086Bifunctional helicase and thymine dioxygenase JBP2

Proteomic databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, compositional bias, domain, motif.

TypeIDPosition(s)Description
Region1-518Thymine dioxygenase
Region187-220Disordered
Compositional bias192-215Polar residues
Region519-1084DNA Helicase
Domain533-708Helicase ATP-binding
Motif659-662DEAH box
Domain883-1041Helicase C-terminal

Sequence similarities

In the C-terminal section; belongs to the SNF2/RAD54 helicase family.
In the N-terminal section; belongs to the TET family. JBP2 subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,086
  • Mass (Da)
    122,630
  • Last updated
    2005-09-13 v1
  • Checksum
    ACDD7E746BDE89DE
MPVPSHVSVATLQSVLQTVCSTGEPAIIAPSSFLGELDTVVDEVKRHGMKLASIPHGGITILPPVPISGTELFDFCAEYCKAQTHEERLAVRHKINNHNFLMQDPLLRMPCRQLYNPADYVLRIVHLCSELVSASEEEYHGAYGVAPLLHINPVQDVCGKLRSMFQSGSLYVKPWILEEEEERREMDESNRGVLFNSDSFGNGRGGSSISSSERSVDENDVADEDLTGEEVVDNATDTVVEYLSEKDFDVVTESGIFYDDSGERVHAIYLRGGIKKELCQRAAIAIEEAATTKNLRKAVNGGKTNPETGIVGYYDYLNNPTQRKCRETEFTRKNWSSVVDSCEPFLVALNKLYSECAPTHYKLQRIAIPHHYQLFNTVFSTMTVNRNFRTAVHTDRGDFRSGLAALCVIDGVFEGCHLAIKKLGKAFRLETGDVLFFDTSLEHGNTEVHNFDYCWKRVSVVCYLRNGLMSQICEMERRRWLQKQMLKQRLLDRSRQSVINLNATDPNLPPIYLPGRLLEVLSPVQQAALGFVVDRLSKGNGCVIALTMGLGKTLLSLALCYSHMYDQNPRDVLILAPKIVLTHWTGEKQKWEKYGLVFSHFVVSDGTDSVSFEIALKRYKQQLNGELPRTSHVFVINPEYIRTVLKKLTGFRPSLIIVDEGHRVSSKGSKLKDWLEGLRCTARVILSGTPVQNNAEELYRLIGWINSDVHSVLPPRVFTDLAGTINRYINGDDSALAAAVSAQRYIQEWMCSYVFSVMKTDLPPLNDYIIICGFSSIQRKMLEDHFGMEGIDGLTSIKASEHRPYHLSTHPLCFLGFISGVYKSLNGNHKLTPEAEEELESQEYASQLYSLTEDDIGLIDECLSLVNSGFLTEFVGLSGKMTVLISILHSIREKKEKAIIFSQYVGSQDFISRTLTSFDIVSSTIRGRDCHERRRRTIEKFREDEKITCLLLSTQIGAYGLDFTAANHVILWDSWWNPQVESQAIARAYRRNQTRAVIVYRLASEFEDTIVLKTQIRKLALFRCIMNEEASRAVPPEELLDCVDTEEDEGRRFLWRSLKKSYLEGGAPAVSKVFRHGDTVRSESWS

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias192-215Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AAHK01000656
EMBL· GenBank· DDBJ
EAN90229.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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