Q4DCH3 · JBP2_TRYCC
- ProteinBifunctional helicase and thymine dioxygenase JBP2
- GeneJBP2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1086 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Dioxygenase that catalyzes the first step of DNA base J (beta-d-glucosyl-HOMedU) biosynthesis by converting thymine to 5-hydroxymethyluracil (HOMedU). DNA base J is a hypermodified thymidine residue found in the genome of kinetoplastid parasites, which is localized primarily to repetitive DNA, namely the telomeres, and is implicated in the regulation of antigenic variation. Probably also acts as a DNA helicase. Recognizes and binds specific regions of the genome, hydrolyzes ATP and allows the DNA base J de novo synthesis. Involved in initial synthesis of DNA base J, JBP1 being able to act via the basal level of DNA base J and propagate further synthesis. In contrast to JBP1, it does not specifically bind DNA base J, however it binds chromatin (By similarity).
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
Cofactor
Note: Binds 1 Fe2+ ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 393 | Fe cation (UniProtKB | ChEBI); catalytic; for thymine dioxygenase activity | ||||
Sequence: H | ||||||
Binding site | 395 | Fe cation (UniProtKB | ChEBI); catalytic; for thymine dioxygenase activity | ||||
Sequence: D | ||||||
Binding site | 443 | Fe cation (UniProtKB | ChEBI); catalytic; for thymine dioxygenase activity | ||||
Sequence: H | ||||||
Binding site | 457 | 2-oxoglutarate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 546-553 | ATP (UniProtKB | ChEBI) | ||||
Sequence: LTMGLGKT |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | ATP-dependent chromatin remodeler activity | |
Molecular Function | DNA binding | |
Molecular Function | helicase activity | |
Molecular Function | metal ion binding | |
Molecular Function | thymine dioxygenase activity | |
Biological Process | base J metabolic process |
Keywords
- Molecular function
- Ligand
Names & Taxonomy
Protein names
- Recommended nameBifunctional helicase and thymine dioxygenase JBP2
- Alternative names
Including 2 domains:
- Recommended nameProbable DNA helicase JBP2
- EC number
- Recommended nameThymine dioxygenase JBP2
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Discoba > Euglenozoa > Kinetoplastea > Metakinetoplastina > Trypanosomatida > Trypanosomatidae > Trypanosoma > Schizotrypanum
Accessions
- Primary accessionQ4DCH3
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000377563 | 1-1086 | Bifunctional helicase and thymine dioxygenase JBP2 | |||
Sequence: MPVPSHVSVATLQSVLQTVCSTGEPAIIAPSSFLGELDTVVDEVKRHGMKLASIPHGGITILPPVPISGTELFDFCAEYCKAQTHEERLAVRHKINNHNFLMQDPLLRMPCRQLYNPADYVLRIVHLCSELVSASEEEYHGAYGVAPLLHINPVQDVCGKLRSMFQSGSLYVKPWILEEEEERREMDESNRGVLFNSDSFGNGRGGSSISSSERSVDENDVADEDLTGEEVVDNATDTVVEYLSEKDFDVVTESGIFYDDSGERVHAIYLRGGIKKELCQRAAIAIEEAATTKNLRKAVNGGKTNPETGIVGYYDYLNNPTQRKCRETEFTRKNWSSVVDSCEPFLVALNKLYSECAPTHYKLQRIAIPHHYQLFNTVFSTMTVNRNFRTAVHTDRGDFRSGLAALCVIDGVFEGCHLAIKKLGKAFRLETGDVLFFDTSLEHGNTEVHNFDYCWKRVSVVCYLRNGLMSQICEMERRRWLQKQMLKQRLLDRSRQSVINLNATDPNLPPIYLPGRLLEVLSPVQQAALGFVVDRLSKGNGCVIALTMGLGKTLLSLALCYSHMYDQNPRDVLILAPKIVLTHWTGEKQKWEKYGLVFSHFVVSDGTDSVSFEIALKRYKQQLNGELPRTSHVFVINPEYIRTVLKKLTGFRPSLIIVDEGHRVSSKGSKLKDWLEGLRCTARVILSGTPVQNNAEELYRLIGWINSDVHSVLPPRVFTDLAGTINRYINGDDSALAAAVSAQRYIQEWMCSYVFSVMKTDLPPLNDYIIICGFSSIQRKMLEDHFGMEGIDGLTSIKASEHRPYHLSTHPLCFLGFISGVYKSLNGNHKLTPEAEEELESQEYASQLYSLTEDDIGLIDECLSLVNSGFLTEFVGLSGKMTVLISILHSIREKKEKAIIFSQYVGSQDFISRTLTSFDIVSSTIRGRDCHERRRRTIEKFREDEKITCLLLSTQIGAYGLDFTAANHVILWDSWWNPQVESQAIARAYRRNQTRAVIVYRLASEFEDTIVLKTQIRKLALFRCIMNEEASRAVPPEELLDCVDTEEDEGRRFLWRSLKKSYLEGGAPAVSKVFRHGDTVRSESWS |
Proteomic databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-518 | Thymine dioxygenase | ||||
Sequence: MPVPSHVSVATLQSVLQTVCSTGEPAIIAPSSFLGELDTVVDEVKRHGMKLASIPHGGITILPPVPISGTELFDFCAEYCKAQTHEERLAVRHKINNHNFLMQDPLLRMPCRQLYNPADYVLRIVHLCSELVSASEEEYHGAYGVAPLLHINPVQDVCGKLRSMFQSGSLYVKPWILEEEEERREMDESNRGVLFNSDSFGNGRGGSSISSSERSVDENDVADEDLTGEEVVDNATDTVVEYLSEKDFDVVTESGIFYDDSGERVHAIYLRGGIKKELCQRAAIAIEEAATTKNLRKAVNGGKTNPETGIVGYYDYLNNPTQRKCRETEFTRKNWSSVVDSCEPFLVALNKLYSECAPTHYKLQRIAIPHHYQLFNTVFSTMTVNRNFRTAVHTDRGDFRSGLAALCVIDGVFEGCHLAIKKLGKAFRLETGDVLFFDTSLEHGNTEVHNFDYCWKRVSVVCYLRNGLMSQICEMERRRWLQKQMLKQRLLDRSRQSVINLNATDPNLPPIYLPGRLL | ||||||
Region | 187-220 | Disordered | ||||
Sequence: DESNRGVLFNSDSFGNGRGGSSISSSERSVDEND | ||||||
Compositional bias | 192-215 | Polar residues | ||||
Sequence: GVLFNSDSFGNGRGGSSISSSERS | ||||||
Region | 519-1084 | DNA Helicase | ||||
Sequence: EVLSPVQQAALGFVVDRLSKGNGCVIALTMGLGKTLLSLALCYSHMYDQNPRDVLILAPKIVLTHWTGEKQKWEKYGLVFSHFVVSDGTDSVSFEIALKRYKQQLNGELPRTSHVFVINPEYIRTVLKKLTGFRPSLIIVDEGHRVSSKGSKLKDWLEGLRCTARVILSGTPVQNNAEELYRLIGWINSDVHSVLPPRVFTDLAGTINRYINGDDSALAAAVSAQRYIQEWMCSYVFSVMKTDLPPLNDYIIICGFSSIQRKMLEDHFGMEGIDGLTSIKASEHRPYHLSTHPLCFLGFISGVYKSLNGNHKLTPEAEEELESQEYASQLYSLTEDDIGLIDECLSLVNSGFLTEFVGLSGKMTVLISILHSIREKKEKAIIFSQYVGSQDFISRTLTSFDIVSSTIRGRDCHERRRRTIEKFREDEKITCLLLSTQIGAYGLDFTAANHVILWDSWWNPQVESQAIARAYRRNQTRAVIVYRLASEFEDTIVLKTQIRKLALFRCIMNEEASRAVPPEELLDCVDTEEDEGRRFLWRSLKKSYLEGGAPAVSKVFRHGDTVRSES | ||||||
Domain | 533-708 | Helicase ATP-binding | ||||
Sequence: VDRLSKGNGCVIALTMGLGKTLLSLALCYSHMYDQNPRDVLILAPKIVLTHWTGEKQKWEKYGLVFSHFVVSDGTDSVSFEIALKRYKQQLNGELPRTSHVFVINPEYIRTVLKKLTGFRPSLIIVDEGHRVSSKGSKLKDWLEGLRCTARVILSGTPVQNNAEELYRLIGWINSD | ||||||
Motif | 659-662 | DEAH box | ||||
Sequence: DEGH | ||||||
Domain | 883-1041 | Helicase C-terminal | ||||
Sequence: VLISILHSIREKKEKAIIFSQYVGSQDFISRTLTSFDIVSSTIRGRDCHERRRRTIEKFREDEKITCLLLSTQIGAYGLDFTAANHVILWDSWWNPQVESQAIARAYRRNQTRAVIVYRLASEFEDTIVLKTQIRKLALFRCIMNEEASRAVPPEELLD |
Sequence similarities
In the C-terminal section; belongs to the SNF2/RAD54 helicase family.
In the N-terminal section; belongs to the TET family. JBP2 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,086
- Mass (Da)122,630
- Last updated2005-09-13 v1
- ChecksumACDD7E746BDE89DE
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 192-215 | Polar residues | ||||
Sequence: GVLFNSDSFGNGRGGSSISSSERS |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AAHK01000656 EMBL· GenBank· DDBJ | EAN90229.1 EMBL· GenBank· DDBJ | Genomic DNA |