Q0V9G6 · INSI1_XENTR
- ProteinInsulin-induced gene 1 protein
- Geneinsig1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids251 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score2/5
Function
function
Oxysterol-binding protein that mediates feedback control of cholesterol synthesis by controlling both endoplasmic reticulum to Golgi transport of scap and degradation of hmgcr. Acts as a negative regulator of cholesterol biosynthesis by mediating the retention of the SCAP-SREBP complex in the endoplasmic reticulum, thereby blocking the processing of sterol regulatory element-binding proteins (SREBPs). Binds oxysterol, including 25-hydroxycholesterol, regulating interaction with scap and retention of the SCAP-SREBP complex in the endoplasmic reticulum. In presence of oxysterol, interacts with scap, retaining the SCAP-SREBP complex in the endoplasmic reticulum, thereby preventing scap from escorting SREBPs to the Golgi. Sterol deprivation reduces oxysterol-binding, disrupting the interaction between insig1 and scap, thereby promoting Golgi transport of the SCAP-SREBP complex, followed by processing and nuclear translocation of SREBPs. Also regulates cholesterol synthesis by regulating degradation of hmgcr.
Features
Showing features for site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 145 | Required for the recognition of 25-hydroxycholesterol | ||||
Sequence: F |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Molecular Function | oxysterol binding | |
Biological Process | cholesterol metabolic process |
Keywords
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameInsulin-induced gene 1 protein
- Short namesINSIG-1
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Amphibia > Batrachia > Anura > Pipoidea > Pipidae > Xenopodinae > Xenopus > Silurana
Accessions
- Primary accessionQ0V9G6
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-58 | Cytoplasmic | ||||
Sequence: MQTLEEHCWSCSCTRGRDKKGTRLSTWLAQRAAKAMSSLNSLLSLAYHTLASSEGRSL | ||||||
Transmembrane | 59-81 | Helical; Name=1 | ||||
Sequence: IRRSLVLFAVGVFLALVLNLLQI | ||||||
Topological domain | 82-100 | Extracellular | ||||
Sequence: QRNVTLFPEEVIATIFSSA | ||||||
Transmembrane | 101-118 | Helical; Name=2 | ||||
Sequence: WWVPPCCGTAAAVVGLLY | ||||||
Topological domain | 119-133 | Cytoplasmic | ||||
Sequence: PCIDSHLGEPHKFKR | ||||||
Transmembrane | 134-156 | Helical; Name=3 | ||||
Sequence: EWASVMRCIAVFVGINHASAKLD | ||||||
Topological domain | 157-159 | Extracellular | ||||
Sequence: FAN | ||||||
Transmembrane | 160-178 | Helical; Name=4 | ||||
Sequence: NVQLSLTLAALSLGLWWTF | ||||||
Topological domain | 179-183 | Cytoplasmic | ||||
Sequence: DRSRS | ||||||
Transmembrane | 184-205 | Helical; Name=5 | ||||
Sequence: GLGLGITIAFLATLITQFLVYN | ||||||
Topological domain | 206-219 | Extracellular | ||||
Sequence: GVYQYTSPDFLYIR | ||||||
Transmembrane | 220-237 | Helical; Name=6 | ||||
Sequence: SWLPCIFFSGGVTVGNIG | ||||||
Topological domain | 238-251 | Cytoplasmic | ||||
Sequence: RQLAMGSSEKTHSD |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000287391 | 1-251 | Insulin-induced gene 1 protein | |||
Sequence: MQTLEEHCWSCSCTRGRDKKGTRLSTWLAQRAAKAMSSLNSLLSLAYHTLASSEGRSLIRRSLVLFAVGVFLALVLNLLQIQRNVTLFPEEVIATIFSSAWWVPPCCGTAAAVVGLLYPCIDSHLGEPHKFKREWASVMRCIAVFVGINHASAKLDFANNVQLSLTLAALSLGLWWTFDRSRSGLGLGITIAFLATLITQFLVYNGVYQYTSPDFLYIRSWLPCIFFSGGVTVGNIGRQLAMGSSEKTHSD |
Proteomic databases
Expression
Gene expression databases
Interaction
Subunit
Interacts with scap; interaction is direct and only takes place in the presence of sterols; it prevents interaction between scap and the coat protein complex II (COPII). Associates with the SCAP-SREBP complex; association is mediated via its interaction with scap and only takes place in the presence of sterols.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 245-251 | KxHxx | ||||
Sequence: SEKTHSD |
Domain
The KxHxx motif mediates association with the coatomer complex.
Binds oxysterols in a pocket within their transmembrane domains and interacts with SCAP via transmembrane domains 3 and 4.
Sequence similarities
Belongs to the INSIG family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length251
- Mass (Da)27,658
- Last updated2006-09-05 v1
- Checksum8556DF21F909E1EA
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BC121578 EMBL· GenBank· DDBJ | AAI21579.1 EMBL· GenBank· DDBJ | mRNA |