Q04TZ4 · MNMA_LEPBJ
- ProteintRNA-specific 2-thiouridylase MnmA
- GenemnmA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids377 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.
Catalytic activity
- AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine34 in tRNA = 2-thiouridine34 in tRNA + A + AMP + diphosphate + H+ + L-cysteinyl-[protein]
CHEBI:17499 + CHEBI:30616 + RHEA-COMP:11726 + RHEA-COMP:11727 CHEBI:65315 Position: 34= RHEA-COMP:11728 CHEBI:87170 Position: 34+ CHEBI:13193 + CHEBI:456215 + CHEBI:33019 + CHEBI:15378 + RHEA-COMP:10131
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 9-16 | ATP (UniProtKB | ChEBI) | ||||
Sequence: AMSGGVDS | ||||||
Binding site | 35 | ATP (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Active site | 105 | Nucleophile | ||||
Sequence: C | ||||||
Binding site | 129 | ATP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Site | 130 | Interaction with tRNA | ||||
Sequence: H | ||||||
Active site | 201 | Cysteine persulfide intermediate | ||||
Sequence: C | ||||||
Site | 339 | Interaction with tRNA | ||||
Sequence: Q |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | tRNA binding | |
Molecular Function | tRNA-uridine 2-sulfurtransferase activity | |
Biological Process | tRNA modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-specific 2-thiouridylase MnmA
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Spirochaetota > Spirochaetia > Leptospirales > Leptospiraceae > Leptospira
Accessions
- Primary accessionQ04TZ4
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000349684 | 1-377 | tRNA-specific 2-thiouridylase MnmA | |||
Sequence: MSKGKIIVAMSGGVDSAVTAGLLMEEGYEVIGVNLRTWEYEAPVCDTTKKSCCSPEDIRDARDVGLSLKIPFYVVKMEKVFQEKVIDRFIDDYQHGKTPNPCVECNTFVKFGALFEKAKALGIDKIATGHYARIVRNGERYAISNGVDIGKNQAYYLYGLSQENLKNVTFPLGGMTKPEVREIARRMGLSVADKAESQEICFIPENDYRKFLEKKHVEFTPGFFKLRDGRIIGKHKGRENFTIGQRKGLGIAWRNPLYVIAIEDDGSVILGEENETYAESFSVIDVNYQGFAPLGEGESFECRVQVRYRHTPIRCRITKMNEDLVVNPLEEVRGVTPGQSAVFYPLNSDYLLLGGIIRKGSIRMQVAKEESAIAFRS | ||||||
Disulfide bond | 105↔201 | Alternate | ||||
Sequence: CNTFVKFGALFEKAKALGIDKIATGHYARIVRNGERYAISNGVDIGKNQAYYLYGLSQENLKNVTFPLGGMTKPEVREIARRMGLSVADKAESQEIC |
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 151-153 | Interaction with tRNA | ||||
Sequence: KNQ | ||||||
Region | 307-308 | Interaction with tRNA | ||||
Sequence: RY |
Sequence similarities
Belongs to the MnmA/TRMU family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length377
- Mass (Da)42,346
- Last updated2006-11-14 v1
- Checksum14857BD9087EE2B4
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000350 EMBL· GenBank· DDBJ | ABJ75626.1 EMBL· GenBank· DDBJ | Genomic DNA |