P9WF00 · ACUF_ASPA1

Function

function

Oxidoreductase; part of the gene cluster that mediates the biosynthesis of aculins (PubMed:26374386).
The pathway begins with the synthesis of 6-methylsalicylic acid by the polyketide synthase (PKS) acuA via condensation of acetate and malonate units (PubMed:26374386).
The 6-methylsalicylic acid decarboxylase acuB then catalyzes the decarboxylation of 6-methylsalicylic acid to yield m-cresol (also known as 3-methylphenol) (Probable). These first reactions occur in the cytosol (By similarity).
The intermediate m-cresol is then transported into the endoplasmic reticulum where the cytochrome P450 monooxygenase acuC converts it to m-hydroxybenzyl alcohol, which is further converted to gentisyl alcohol by the cytochrome P450 monooxygenase acuD (Probable). Gentisyl alcohol is further oxidized by the oxidoreductase acuE that probably catalyzes hydroxylation of the aromatic ring (Probable). The aromatic system might then be opened by oxidation through a Baeyer-Villiger type of oxidation, which could be catalyzed by acuF, with the carboxylic acid at C-1 subsequently reduced to an aldehyde by acuG (Probable). Subsequently, a hemiacetal is formed, before the dehydrogenase acuH would reduce the double bond between C-4 and C-6 (Probable). Finally, keto-enol tautomerism results in formation of aculinic acid, which exists as two diastereomers (both R/S configurations at C-1) by non-enzymatic hemiacetal formation (Probable). The carboxypeptidase acuI could be involved in the linking of aculinic acid to an aculene A moiety produced by the aculene biosynthesis cluster and which leads to the production of aculin A (Probable). AcuI may also be involved in the attachment of proline to aculinic acid to form epi-aculins A and B (Probable)

Pathway

Secondary metabolite biosynthesis.

GO annotations

AspectTerm
Molecular Functionoxidoreductase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Oxidoreductase acuF
  • EC number
  • Alternative names
    • Aculin biosynthesis cluster protein F

Gene names

    • Name
      acuF

Organism names

Accessions

  • Primary accession
    P9WF00

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004504211-433Oxidoreductase acuF

Family & Domains

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    433
  • Mass (Da)
    49,108
  • Last updated
    2020-08-12 v1
  • Checksum
    7EA548E084FCF20F
MERAISTTPILDQALTCVQSYKALAATVQELASPELQRSIEEGLAQFLTHMPEFNILVNAVEPPHTSWDYLLRNDASQRGEMTMLLMELQCEIDQMAFAIRQAMQQQHPEAEPLSNNSRLVDAWTENLRSVEDHTVWRLAFWRRDHAVHTTYPTTNPLVNQSFYFAIRNELGLDVSTQFRECIRRAITQECPTITARPALHRRLVDLVVQRRRFWNFQRRIRGLSKDAVLWGRDALAPPHFAPGDERFTCPFCFFDLPVAAYRTPRAWTDHVMSDLEGYVCLYETCSSTRSWPETCPHACTSFGAWFTAMAGSFAEGVEWCSHAFACDGRRLEEVHRPRCAAPAVRLDSVCPLCGREPAVEETELRMADAADPLQDDDQNRAKARLLLSHIAGHLEVITPMAFTWNTDRNALDPDRRLFWGDIGSPGGAGGSA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KV878982
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp