P97479 · MYO7A_MOUSE
- ProteinUnconventional myosin-VIIa
- GeneMyo7a
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids2215 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. In the retina, plays an important role in the renewal of the outer photoreceptor disks. Plays an important role in the distribution and migration of retinal pigment epithelial (RPE) melanosomes and phagosomes, and in the regulation of opsin transport in retinal photoreceptors. Mediates intracellular transport of RPE65 in the retina pigment epithelium. In the inner ear, plays an important role in differentiation, morphogenesis and organization of cochlear hair cell bundles. Motor protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal hearing. Involved in hair-cell vesicle trafficking of aminoglycosides, which are known to induce ototoxicity.
Features
Showing features for binding site.
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameUnconventional myosin-VIIa
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionP97479
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: In the photoreceptor cells, mainly localized in the inner and base of outer segments as well as in the synaptic ending region (By similarity).
In retinal pigment epithelial cells colocalizes with a subset of melanosomes, displays predominant localization to stress fiber-like structures and some localization to cytoplasmic puncta (By similarity).
Detected at the tip of cochlear hair cell stereocilia (PubMed:27525485).
The complex formed by MYO7A, USH1C and USH1G colocalizes with F-actin (By similarity).
In retinal pigment epithelial cells colocalizes with a subset of melanosomes, displays predominant localization to stress fiber-like structures and some localization to cytoplasmic puncta (By similarity).
Detected at the tip of cochlear hair cell stereocilia (PubMed:27525485).
The complex formed by MYO7A, USH1C and USH1G colocalizes with F-actin (By similarity).
Keywords
- Cellular component
Phenotypes & Variants
Involvement in disease
Features
Showing features for natural variant, mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | 241 | in sh-1 | ||||
Sequence: R → P | ||||||
Natural variant | 502 | in sh-1 | ||||
Sequence: R → P | ||||||
Mutagenesis | 1189 | Strongly reduced affinity for USH1G. | ||||
Sequence: A → E | ||||||
Mutagenesis | 1473 | Reduced affinity for USH1G. | ||||
Sequence: F → Q |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 105 variants from UniProt as well as other sources including ClinVar and dbSNP.
Keywords
- Disease
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000123467 | 1-2215 | Unconventional myosin-VIIa | |||
Sequence: MVILQKGDYVWMDLKSGQEFDVPIGAVVKLCDSGQIQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNNRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQAPDERNYHVFYCMLEGMNEEEKKKLGLGQAADYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHMGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVEKINAAIYKPPPLEVKNSRRSIGLLDIFGFENFTVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQEALDMIANRPMNVISLIDEESKFPKGTDATMLHKLNSQHKLNANYVPPKNSHETQFGINHFAGVVYYESQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRHAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLRLKSAATLIQRHWRGHHCRKNYELIRLGFLRLQALHRSRKLHKQYRLARQRIIEFQARCRAYLVRKAFRHRLWAVITVQAYARGMIARRLHRRLRVEYQRRLEAERMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEEARRKKELLEQMEKARHEPINHSDMVDKMFGFLGTSGSLPGQEGQAPSGFEDLERGRREMVEEDVDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGETQLPEGQKKTSVRHKLVHLTLKKKSKLTEEVTKRLNDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTARELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHNPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLSLVPTYIPDREITPLKNLEKWAQLAIAAHKKGIYAQRRTDSQKVKEDVVNYARFKWPLLFSRFYEAYKFSGPPLPKSDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRECRVLLSLGCSDLGCATCQSGRAGLTPAGPCSPCWSCRGTKMMAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDCVYVMPTVTLPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKILGSEELSQEACMAFVAVLKYMGDYPSKRMRSVNELTDQIFEWALKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIASRLLLKSSEGFSLFVKIADKVISVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPRTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRNSRSGR | ||||||
Modified residue | 1563 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 1569 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 1571 | Phosphothreonine | ||||
Sequence: T |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Detected in mechanosensory stereocilia of cochlea hair cells (at protein level). Expressed in the retina, cochlea, kidney and liver.
Developmental stage
In the inner ear of the 16.5 day old embryo, expressed only in the cochlear and vestibular sensory hair cells. In addition, expression also occurs in the epithelial cells of the small intestine, hepatocytes, and choroidal plexus.
Gene expression databases
Interaction
Subunit
Might homodimerize in a two headed molecule through the formation of a coiled-coil rod (By similarity).
Identified in a complex with USH1C and USH1G (By similarity).
Interacts with MYRIP (PubMed:12221080).
Interacts with RPE65 (PubMed:21493626).
Interacts with CIB2 (By similarity).
May interact with CALM (By similarity).
Interacts with WHRN (PubMed:15590698).
Interacts with PLEKHB1 (via PH domain) (PubMed:15976448).
Interacts with PCDH15 (PubMed:16481439).
Interacts with TWF2 (PubMed:19774077).
Interacts with USH1G (PubMed:21311020).
Interacts with MYH9 (PubMed:27331610).
Interacts (via MyTH4-FERM domains) with cytoplasmic regions of ADGRV1 and USH2A (PubMed:17567809).
Interacts with PDZD7 (via MyTH4-FERM domains) (PubMed:27525485).
Interacts with CALML4 (By similarity).
Identified in a complex with USH1C and USH1G (By similarity).
Interacts with MYRIP (PubMed:12221080).
Interacts with RPE65 (PubMed:21493626).
Interacts with CIB2 (By similarity).
May interact with CALM (By similarity).
Interacts with WHRN (PubMed:15590698).
Interacts with PLEKHB1 (via PH domain) (PubMed:15976448).
Interacts with PCDH15 (PubMed:16481439).
Interacts with TWF2 (PubMed:19774077).
Interacts with USH1G (PubMed:21311020).
Interacts with MYH9 (PubMed:27331610).
Interacts (via MyTH4-FERM domains) with cytoplasmic regions of ADGRV1 and USH2A (PubMed:17567809).
Interacts with PDZD7 (via MyTH4-FERM domains) (PubMed:27525485).
Interacts with CALML4 (By similarity).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P97479 | Itgb5 O70309 | 3 | EBI-1149557, EBI-8401821 | |
XENO | P97479 | ITGB5 P18084 | 8 | EBI-1149557, EBI-1223434 | |
BINARY | P97479 | Rpe65 Q91ZQ5 | 3 | EBI-1149557, EBI-11682496 | |
BINARY | P97479 | Ush1g Q80T11 | 4 | EBI-1149557, EBI-7418889 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 65-741 | Myosin motor | ||||
Sequence: HGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNNRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQAPDERNYHVFYCMLEGMNEEEKKKLGLGQAADYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHMGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVEKINAAIYKPPPLEVKNSRRSIGLLDIFGFENFTVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQEALDMIANRPMNVISLIDEESKFPKGTDATMLHKLNSQHKLNANYVPPKNSHETQFGINHFAGVVYYESQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRHAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERD | ||||||
Region | 632-639 | Actin-binding | ||||
Sequence: FVRCIKPN | ||||||
Domain | 745-765 | IQ 1 | ||||
Sequence: TDRVILLQKVIRGFKDRSNFL | ||||||
Domain | 768-788 | IQ 2 | ||||
Sequence: KSAATLIQRHWRGHHCRKNYE | ||||||
Domain | 791-811 | IQ 3 | ||||
Sequence: RLGFLRLQALHRSRKLHKQYR | ||||||
Domain | 814-834 | IQ 4 | ||||
Sequence: RQRIIEFQARCRAYLVRKAFR | ||||||
Domain | 837-857 | IQ 5 | ||||
Sequence: LWAVITVQAYARGMIARRLHR | ||||||
Region | 858-935 | SAH | ||||
Sequence: RLRVEYQRRLEAERMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEEARRKKELLEQMEKARHE | ||||||
Domain | 1017-1253 | MyTH4 1 | ||||
Sequence: YTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGETQLPEGQKKTSVRHKLVHLTLKKKSKLTEEVTKRLNDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATK | ||||||
Domain | 1258-1602 | FERM 1 | ||||
Sequence: IMLPVTFMDGTTKTLLTDSATTARELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHNPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLSLVPTYIPDREITPLKNLEKWAQLAIAAHKKGIYAQRRTDSQKVKEDVVNYARFKWPLLFSRFYEAYKFSGPPLPKSDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRECRVLLSLGCSDLGCATCQSGRAGLTPAGPCSPCWSCRGTKMMAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLR | ||||||
Domain | 1603-1672 | SH3 | ||||
Sequence: KRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDCVYVMPT | ||||||
Domain | 1747-1896 | MyTH4 2 | ||||
Sequence: HTREPLKQALLKKILGSEELSQEACMAFVAVLKYMGDYPSKRMRSVNELTDQIFEWALKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQ | ||||||
Domain | 1902-2205 | FERM 2 | ||||
Sequence: IFHKVYFPDDTDEAFEVESSTKAKDFCQNIASRLLLKSSEGFSLFVKIADKVISVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPRTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAM |
Domain
The SAH (single alpha-helix) region is characterized by a high content of charged residues which are predicted to stabilize the alpha-helical structure by ionic bonds.
Sequence similarities
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
P97479-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length2,215
- Mass (Da)254,939
- Last updated2011-07-27 v2
- ChecksumD942FC7674B75EC7
P97479-2
- Name2
- Differences from canonical
- 1523-1560: Missing
Computationally mapped potential isoform sequences
There are 7 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
Q5MJ56 | Q5MJ56_MOUSE | Myo7a | 2172 | ||
A0A0R4J113 | A0A0R4J113_MOUSE | Myo7a | 2166 | ||
A0A0U1RPX7 | A0A0U1RPX7_MOUSE | Myo7a | 2164 | ||
A0A0U1RPT3 | A0A0U1RPT3_MOUSE | Myo7a | 215 | ||
D3YUT5 | D3YUT5_MOUSE | Myo7a | 139 | ||
A0A571BE72 | A0A571BE72_MOUSE | Myo7a | 100 | ||
A0A571BEN3 | A0A571BEN3_MOUSE | Myo7a | 76 |
Features
Showing features for sequence conflict, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 85 | in Ref. 1; AAB40708 | ||||
Sequence: L → R | ||||||
Sequence conflict | 238 | in Ref. 1; AAB40708 | ||||
Sequence: E → S | ||||||
Sequence conflict | 589 | in Ref. 1; AAB40708 | ||||
Sequence: I → V | ||||||
Sequence conflict | 682 | in Ref. 1; AAB40708 | ||||
Sequence: V → G | ||||||
Sequence conflict | 1156 | in Ref. 1; AAB40708 | ||||
Sequence: F → L | ||||||
Alternative sequence | VSP_042238 | 1523-1560 | in isoform 2 | |||
Sequence: Missing |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U81453 EMBL· GenBank· DDBJ | AAB40708.1 EMBL· GenBank· DDBJ | mRNA | ||
AC115022 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AC119880 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AC157792 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AY821853 EMBL· GenBank· DDBJ | AAV87212.1 EMBL· GenBank· DDBJ | mRNA |