P97479 · MYO7A_MOUSE

  • Protein
    Unconventional myosin-VIIa
  • Gene
    Myo7a
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. In the retina, plays an important role in the renewal of the outer photoreceptor disks. Plays an important role in the distribution and migration of retinal pigment epithelial (RPE) melanosomes and phagosomes, and in the regulation of opsin transport in retinal photoreceptors. Mediates intracellular transport of RPE65 in the retina pigment epithelium. In the inner ear, plays an important role in differentiation, morphogenesis and organization of cochlear hair cell bundles. Motor protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal hearing. Involved in hair-cell vesicle trafficking of aminoglycosides, which are known to induce ototoxicity.

Caution

Represents an unconventional myosin. This protein should not be confused with the conventional myosin-7 (MYH7).
Originally predicted to contain a coiled coil domain but proposed to contain a stable SAH domain instead.

Features

Showing features for binding site.

122152004006008001,0001,2001,4001,6001,8002,0002,200
TypeIDPosition(s)Description
Binding site158-165ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentactin cytoskeleton
Cellular Componentapical plasma membrane
Cellular Componentcell cortex
Cellular Componentcytoplasm
Cellular Componentcytosol
Cellular Componentlysosomal membrane
Cellular Componentmelanosome
Cellular Componentmembrane
Cellular Componentmicrovillus
Cellular Componentmyosin complex
Cellular Componentmyosin VII complex
Cellular Componentphotoreceptor connecting cilium
Cellular Componentphotoreceptor inner segment
Cellular Componentphotoreceptor outer segment
Cellular Componentstereocilium
Cellular Componentstereocilium base
Cellular Componentsynapse
Cellular Componentupper tip-link density
Molecular Functionactin binding
Molecular Functionactin filament binding
Molecular FunctionADP binding
Molecular FunctionATP binding
Molecular Functioncalmodulin binding
Molecular Functioncytoskeletal motor activity
Molecular Functionidentical protein binding
Molecular Functionmicrofilament motor activity
Molecular Functionprotein domain specific binding
Molecular Functionprotein-containing complex binding
Molecular Functionspectrin binding
Biological Processactin filament organization
Biological Processactin filament-based movement
Biological Processauditory receptor cell stereocilium organization
Biological Processcell projection organization
Biological Processcochlea development
Biological Processendocytosis
Biological Processequilibrioception
Biological Processinner ear auditory receptor cell differentiation
Biological Processinner ear development
Biological Processinner ear morphogenesis
Biological Processinner ear receptor cell differentiation
Biological Processinner ear receptor cell stereocilium organization
Biological Processintracellular protein transport
Biological Processlysosome organization
Biological Processmechanoreceptor differentiation
Biological Processphagocytosis
Biological Processphagolysosome assembly
Biological Processpigment granule localization
Biological Processpigment granule transport
Biological Processprotein localization
Biological Processsensory organ development
Biological Processsensory perception
Biological Processsensory perception of light stimulus
Biological Processsensory perception of sound
Biological Processvisual perception

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Unconventional myosin-VIIa

Gene names

    • Name
      Myo7a
    • Synonyms
      Myo7

Organism names

  • Taxonomic identifier
  • Strain
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    P97479
  • Secondary accessions
    • E9QLP7
    • Q5MJ57

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasm
Synapse
Note: In the photoreceptor cells, mainly localized in the inner and base of outer segments as well as in the synaptic ending region (By similarity).
In retinal pigment epithelial cells colocalizes with a subset of melanosomes, displays predominant localization to stress fiber-like structures and some localization to cytoplasmic puncta (By similarity).
Detected at the tip of cochlear hair cell stereocilia (PubMed:27525485).
The complex formed by MYO7A, USH1C and USH1G colocalizes with F-actin (By similarity).

Keywords

Phenotypes & Variants

Involvement in disease

  • Defects in Myo7a are the cause of the shaker-1 (sh-1) phenotype which affects only the inner ear. Sh-1 homozygote mutants show hyperactivity, head tossing and circling due to vestibular dysfunction, together with typical neuroepithelial-type cochlear defects involving dysfunction and progressive degeneration of the organ of Corti

Features

Showing features for natural variant, mutagenesis.

TypeIDPosition(s)Description
Natural variant241in sh-1
Natural variant502in sh-1
Mutagenesis1189Strongly reduced affinity for USH1G.
Mutagenesis1473Reduced affinity for USH1G.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 105 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

Keywords

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00001234671-2215Unconventional myosin-VIIa
Modified residue1563Phosphothreonine
Modified residue1569Phosphoserine
Modified residue1571Phosphothreonine

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Detected in mechanosensory stereocilia of cochlea hair cells (at protein level). Expressed in the retina, cochlea, kidney and liver.

Developmental stage

In the inner ear of the 16.5 day old embryo, expressed only in the cochlear and vestibular sensory hair cells. In addition, expression also occurs in the epithelial cells of the small intestine, hepatocytes, and choroidal plexus.

Gene expression databases

Interaction

Subunit

Might homodimerize in a two headed molecule through the formation of a coiled-coil rod (By similarity).
Identified in a complex with USH1C and USH1G (By similarity).
Interacts with MYRIP (PubMed:12221080).
Interacts with RPE65 (PubMed:21493626).
Interacts with CIB2 (By similarity).
May interact with CALM (By similarity).
Interacts with WHRN (PubMed:15590698).
Interacts with PLEKHB1 (via PH domain) (PubMed:15976448).
Interacts with PCDH15 (PubMed:16481439).
Interacts with TWF2 (PubMed:19774077).
Interacts with USH1G (PubMed:21311020).
Interacts with MYH9 (PubMed:27331610).
Interacts (via MyTH4-FERM domains) with cytoplasmic regions of ADGRV1 and USH2A (PubMed:17567809).
Interacts with PDZD7 (via MyTH4-FERM domains) (PubMed:27525485).
Interacts with CALML4 (By similarity).

Binary interactions

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain65-741Myosin motor
Region632-639Actin-binding
Domain745-765IQ 1
Domain768-788IQ 2
Domain791-811IQ 3
Domain814-834IQ 4
Domain837-857IQ 5
Region858-935SAH
Domain1017-1253MyTH4 1
Domain1258-1602FERM 1
Domain1603-1672SH3
Domain1747-1896MyTH4 2
Domain1902-2205FERM 2

Domain

The SAH (single alpha-helix) region is characterized by a high content of charged residues which are predicted to stabilize the alpha-helical structure by ionic bonds.

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

P97479-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    2,215
  • Mass (Da)
    254,939
  • Last updated
    2011-07-27 v2
  • Checksum
    D942FC7674B75EC7
MVILQKGDYVWMDLKSGQEFDVPIGAVVKLCDSGQIQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNNRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQAPDERNYHVFYCMLEGMNEEEKKKLGLGQAADYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHMGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVEKINAAIYKPPPLEVKNSRRSIGLLDIFGFENFTVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQEALDMIANRPMNVISLIDEESKFPKGTDATMLHKLNSQHKLNANYVPPKNSHETQFGINHFAGVVYYESQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRHAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLRLKSAATLIQRHWRGHHCRKNYELIRLGFLRLQALHRSRKLHKQYRLARQRIIEFQARCRAYLVRKAFRHRLWAVITVQAYARGMIARRLHRRLRVEYQRRLEAERMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEEARRKKELLEQMEKARHEPINHSDMVDKMFGFLGTSGSLPGQEGQAPSGFEDLERGRREMVEEDVDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGETQLPEGQKKTSVRHKLVHLTLKKKSKLTEEVTKRLNDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTARELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHNPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLSLVPTYIPDREITPLKNLEKWAQLAIAAHKKGIYAQRRTDSQKVKEDVVNYARFKWPLLFSRFYEAYKFSGPPLPKSDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRECRVLLSLGCSDLGCATCQSGRAGLTPAGPCSPCWSCRGTKMMAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDCVYVMPTVTLPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKILGSEELSQEACMAFVAVLKYMGDYPSKRMRSVNELTDQIFEWALKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIASRLLLKSSEGFSLFVKIADKVISVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPRTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRNSRSGR

P97479-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Computationally mapped potential isoform sequences

There are 7 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
Q5MJ56Q5MJ56_MOUSEMyo7a2172
A0A0R4J113A0A0R4J113_MOUSEMyo7a2166
A0A0U1RPX7A0A0U1RPX7_MOUSEMyo7a2164
A0A0U1RPT3A0A0U1RPT3_MOUSEMyo7a215
D3YUT5D3YUT5_MOUSEMyo7a139
A0A571BE72A0A571BE72_MOUSEMyo7a100
A0A571BEN3A0A571BEN3_MOUSEMyo7a76

Features

Showing features for sequence conflict, alternative sequence.

TypeIDPosition(s)Description
Sequence conflict85in Ref. 1; AAB40708
Sequence conflict238in Ref. 1; AAB40708
Sequence conflict589in Ref. 1; AAB40708
Sequence conflict682in Ref. 1; AAB40708
Sequence conflict1156in Ref. 1; AAB40708
Alternative sequenceVSP_0422381523-1560in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U81453
EMBL· GenBank· DDBJ
AAB40708.1
EMBL· GenBank· DDBJ
mRNA
AC115022
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
AC119880
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
AC157792
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
AY821853
EMBL· GenBank· DDBJ
AAV87212.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp