P96178 · RPOC_WEIPA
- ProteinDNA-directed RNA polymerase subunit beta'
- GenerpoC
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1054 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity
- a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 Mg2+ ion per subunit.
Note: Binds 1 Zn2+ ion1 per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 383 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 385 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 387 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 752 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 826 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 833 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 836 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | DNA-directed RNA polymerase complex | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Molecular Function | metal ion binding | |
Biological Process | DNA-templated transcription |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase subunit beta'
- EC number
- Short namesRNAP subunit beta'
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Lactobacillales > Lactobacillaceae > Weissella
Accessions
- Primary accessionP96178
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000067832 | 1-1054 | DNA-directed RNA polymerase subunit beta' | |||
Sequence: RIRYKGIVCDRCGVEVTSAKVRRERMGHIELAAPVSHIWYFKGIPSRMGLVLDMSPRSLEEIIYFASYVVTKGGDTPLTEKQLLSEREYRELKAEYGNAFEAGMGAEAVQTLLANVDLEEEVTELKAELREATGQKRVRAVRRLDIIEAFVKSGNKPEWMVMDVIPIIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLELNAPGIIVQNEKRMLQEAADALVDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSVIDVGPFLKMNQMGLPRQMAMELFRPFIMKELVKRDLAGNIRAAKRKIDRRDDDVMDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVSGKAMRLHPLVTEAYNADFDGDQMAIHVPLSDEAQAEARLLMLAAGHILAPKDGKPIVAPSQDMVIGNYYLTTEEAGREGEGMIFKDVNEVRTAYQNKYVHLHTRIGIQTSSLPAAKPFTAEQRSRIMLTSVGKLFFNDILPEDFPFLNEPTEANLHEIDNRFFLEPGEDIKAHYAETPILPPFKKGYLSDIIAEVYKIYKVTETSLLLDRMKDLGYDESTKSGLTVGVSDVTDLKEKPEIIADAHKQVATVTKQFRRGLITDSERYERVIAIWNKAKDDITEKLIEHFEPDNNIFMMSDSGARGNISNFTQLAGMRGLMAAPNGRIMELPIIANFREGLSVLEMFFSTHGARKGMTDTALKTADSGYMTRRLVDVAQDVIIRELDCHSDRGLDVTAIMNGNEVIEPLYERILGRYAQKSVFDPETGETLVNHNEMITEDIAKRIIEAGITTVTIRSAFTCNTEHGVCVRCYGRNMATGDVVEVGEAVGTVAAQSIGEPGTQLTMRTFHTGGVAGNDITQGLPRVQEIFEARNPKGRAMITEVTGEVTSIEENPADRTKEVTIQGETDTRTYTLPMTARMRVGEGDRIHRGETLNEGSADPKEIIQVRDTLATENYIVHEVQKVYRMQGVEISDKHIEVMARQMLRKVRVMDPGETDLLPGTLMDIAQFRDANEDTLLKGGLPATGRPV |
Interaction
Subunit
The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Structure
Sequence
- Sequence statusFragment
- Length1,054
- Mass (Da)118,067
- Last updated1997-05-01 v1
- Checksum1B3E81C060154EEA
Features
Showing features for non-terminal residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 1 | |||||
Sequence: R | ||||||
Non-terminal residue | 1054 | |||||
Sequence: V |