P93198 · 2SS_JUGRE
- Protein2S seed storage albumin protein
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids139 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Seed storage protein.
Biotechnology
The recombinant protein could be used for a reliable component-resolved diagnosis of walnut allergy as it has the same properties and virtually identical IgE-binding reactivity as the native protein even that it is unprocessed and hence lacking the proteolytic cleavages of the mature protein (PubMed:19540419).
A rapid, highly specific and relatively sensitive indirect competitive enzyme-linked immunosorbent assay (ELISA) for detection and quantification of walnut protein component in processed foods has been developed using polyclonal antibodies against this protein (PubMed:24206692).
Testing young patients specific IgE-reactivity of this protein is a better approach in diagnosing clinical walnut allergy in children and youth compared to the walnut extract-based testing due to its improved specificity as shown by the receiver operating characteristic (ROC) statistical analysis (PubMed:28552380, PubMed:31175720).
The strategy of synthesizing digestion-resistant peptides instead of overlapping peptides, and verifying them using sera from walnut-allergic patients, can significantly reduce workload and serum requirement, enhance pertinence, and improve efficiency in studying linear epitopes of this protein (PubMed:32283362).
A rapid, highly specific and relatively sensitive indirect competitive enzyme-linked immunosorbent assay (ELISA) for detection and quantification of walnut protein component in processed foods has been developed using polyclonal antibodies against this protein (PubMed:24206692).
Testing young patients specific IgE-reactivity of this protein is a better approach in diagnosing clinical walnut allergy in children and youth compared to the walnut extract-based testing due to its improved specificity as shown by the receiver operating characteristic (ROC) statistical analysis (PubMed:28552380, PubMed:31175720).
The strategy of synthesizing digestion-resistant peptides instead of overlapping peptides, and verifying them using sera from walnut-allergic patients, can significantly reduce workload and serum requirement, enhance pertinence, and improve efficiency in studying linear epitopes of this protein (PubMed:32283362).
Temperature Dependence
Resistant to heat. Retains its alpha-helical fold after heating at 90 degrees Celsius and subsequent cooling to 20 degrees Celsius.
Features
Showing features for site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 113 | Necessary for maximum IgE-binding by the immunodominant epitope | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extraorganismal space | |
Molecular Function | nutrient reservoir activity | |
Biological Process | seed maturation |
Keywords
- Molecular function
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended name2S seed storage albumin protein
- Alternative names
- Cleaved into 2 chains
- Allergen nameJug r 1.0101
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fagales > Juglandaceae > Juglans
Accessions
- Primary accessionP93198
Proteomes
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Phenotypes & Variants
Allergenic properties
Causes an allergic reaction in human. Binds to IgE of patients allergic to walnuts (PubMed:11799381, PubMed:19540419, PubMed:22042002, PubMed:25388987, PubMed:27597723, PubMed:28552380, PubMed:31175720, PubMed:32283362, PubMed:9648708).
Binds to IgE of patients allergic to peanuts (PubMed:22042002).
Binds to IgE in 97% of the 32 Korean babies and children tested allergic to walnuts (PubMed:31175720).
Native protein binds to IgE in 100% of the 6 Chinese walnut-allergic patients tested (PubMed:32283362).
Native protein binds to IgE in 45% of the 11 walnut-allergic patients tested living in Spain or England (PubMed:25388987).
Recombinant protein binds to IgE in 75% of the 16 walnut-allergic patients tested (PubMed:9648708).
Recombinant protein binds to IgE in 100% of the 20 walnut-allergic patients tested (PubMed:11799381).
Recombinant protein binds to IgE in 55% and 61% of the 33 walnut-allergic adult patients living in Netherlands tested by ImmunoCAP and ImmunoCAP ISAC, respectively (PubMed:27597723).
Contains linear and conformational IgE-binding epitopes (PubMed:11799381).
Cross-reacts with sesame seed allergens Ses i 1 and Ses i 2. IgE-binding is lost in the presence of pepsin after 1 hour incubation at pH 1.5 and 37 degrees Celsius in vitro, but it is not affected in the presence of trypsin and chymotrypsin after 2 hour incubation in vitro at pH 8 and 37 degrees Celsius. Induces in vitro degranulation of the humanized rat basophilic leukemia (RBL) cells and release of beta-hexosaminidase from them (PubMed:19540419).
Binds to IgE of patients allergic to peanuts (PubMed:22042002).
Binds to IgE in 97% of the 32 Korean babies and children tested allergic to walnuts (PubMed:31175720).
Native protein binds to IgE in 100% of the 6 Chinese walnut-allergic patients tested (PubMed:32283362).
Native protein binds to IgE in 45% of the 11 walnut-allergic patients tested living in Spain or England (PubMed:25388987).
Recombinant protein binds to IgE in 75% of the 16 walnut-allergic patients tested (PubMed:9648708).
Recombinant protein binds to IgE in 100% of the 20 walnut-allergic patients tested (PubMed:11799381).
Recombinant protein binds to IgE in 55% and 61% of the 33 walnut-allergic adult patients living in Netherlands tested by ImmunoCAP and ImmunoCAP ISAC, respectively (PubMed:27597723).
Contains linear and conformational IgE-binding epitopes (PubMed:11799381).
Cross-reacts with sesame seed allergens Ses i 1 and Ses i 2. IgE-binding is lost in the presence of pepsin after 1 hour incubation at pH 1.5 and 37 degrees Celsius in vitro, but it is not affected in the presence of trypsin and chymotrypsin after 2 hour incubation in vitro at pH 8 and 37 degrees Celsius. Induces in vitro degranulation of the humanized rat basophilic leukemia (RBL) cells and release of beta-hexosaminidase from them (PubMed:19540419).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 104 | IgE-binding is retained by the immunodominant epitope. | ||||
Sequence: Q → A | ||||||
Mutagenesis | 105 | IgE-binding is retained by the immunodominant epitope. | ||||
Sequence: G → A | ||||||
Mutagenesis | 106 | IgE-binding is retained by the immunodominant epitope. | ||||
Sequence: L → A | ||||||
Mutagenesis | 107 | Loss of IgE-binding by the immunodominant epitope. | ||||
Sequence: R → A | ||||||
Mutagenesis | 108 | Loss of IgE-binding by the immunodominant epitope. | ||||
Sequence: G → A | ||||||
Mutagenesis | 109 | Loss of IgE-binding by the immunodominant epitope. | ||||
Sequence: E → A | ||||||
Mutagenesis | 110 | Loss of IgE-binding by the immunodominant epitope. | ||||
Sequence: E → A | ||||||
Mutagenesis | 111 | IgE-binding is retained by the immunodominant epitope. | ||||
Sequence: M → A | ||||||
Mutagenesis | 112 | IgE-binding is retained by the immunodominant epitope. | ||||
Sequence: E → A | ||||||
Mutagenesis | 113 | IgE-binding is retained by the immunodominant epitope. | ||||
Sequence: E → A | ||||||
Mutagenesis | 114 | IgE-binding is retained by the immunodominant epitope. | ||||
Sequence: M → A | ||||||
Mutagenesis | 115 | IgE-binding is retained by the immunodominant epitope. | ||||
Sequence: V → A |
Keywords
- Disease
Protein family/group databases
PTM/Processing
Features
Showing features for signal, propeptide, chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-15 | |||||
Sequence: AALLVALLFVANAAA | ||||||
Propeptide | PRO_0000451111 | 16-31 | ||||
Sequence: FRTTITTMEIDEDIDN | ||||||
Chain | PRO_0000451112 | 32-57 | 2S albumin seed storage protein small subunit | |||
Sequence: PRRRGEGCREQIQRQQNLNHCQYYLR | ||||||
Disulfide bond | 39↔88 | Interchain (between small and large chains) | ||||
Sequence: CREQIQRQQNLNHCQYYLRQQSRSGGYDEDNQRQHFRQCCQQLSQMDEQC | ||||||
Disulfide bond | 52↔77 | Interchain (between small and large chains) | ||||
Sequence: CQYYLRQQSRSGGYDEDNQRQHFRQC | ||||||
Propeptide | PRO_0000451113 | 58-71 | ||||
Sequence: QQSRSGGYDEDNQR | ||||||
Chain | PRO_0000451114 | 72-135 | 2S albumin seed storage protein large subunit | |||
Sequence: QHFRQCCQQLSQMDEQCQCEGLRQVVRRQQQQQGLRGEEMEEMVQSARDLPNECGISSQRCEIR | ||||||
Disulfide bond | 78↔125 | |||||
Sequence: CQQLSQMDEQCQCEGLRQVVRRQQQQQGLRGEEMEEMVQSARDLPNEC | ||||||
Disulfide bond | 90↔132 | |||||
Sequence: CEGLRQVVRRQQQQQGLRGEEMEEMVQSARDLPNECGISSQRC | ||||||
Propeptide | PRO_0000451115 | 136-139 | ||||
Sequence: RSWF |
Keywords
- PTM
Interaction
Subunit
The mature protein consists of a small chain and a large chain linked by disulfide bonds.
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 16-30 | IgE-binding | ||||
Sequence: FRTTITTMEIDEDID | ||||||
Region | 29-34 | IgE-binding | ||||
Sequence: IDNPRR | ||||||
Region | 64-78 | IgE-binding | ||||
Sequence: GYDEDNQRQHFRQCC | ||||||
Region | 65-73 | IgE-binding | ||||
Sequence: YDEDNQRQH | ||||||
Region | 95-103 | IgE-binding | ||||
Sequence: QVVRRQQQQ | ||||||
Region | 99-111 | IgE-binding | ||||
Sequence: RQQQQQGLRGEEM | ||||||
Region | 102-114 | IgE-binding | ||||
Sequence: QQQGLRGEEMEEM | ||||||
Region | 104-115 | Immunodominant epitope. IgE-binding; binds to IgE in 75% of the 20 walnut-allergic patients tested | ||||
Sequence: QGLRGEEMEEMV | ||||||
Region | 105-117 | IgE-binding | ||||
Sequence: GLRGEEMEEMVQS | ||||||
Region | 107-110 | Minimally required for IgE-binding by the immunodominant epitope | ||||
Sequence: RGEE | ||||||
Region | 112-126 | IgE-binding | ||||
Sequence: EEMVQSARDLPNECG | ||||||
Region | 125-136 | IgE-binding | ||||
Sequence: CGISSQRCEIRR | ||||||
Region | 125-139 | IgE-binding | ||||
Sequence: CGISSQRCEIRRSWF |
Sequence similarities
Belongs to the 2S seed storage albumins family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusFragment
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length139
- Mass (Da)16,373
- Last updated1997-05-01 v1
- Checksum02D0E55E67164F23
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A2I4EX90 | A0A2I4EX90_JUGRE | LOC108993506 | 146 |
Features
Showing features for non-terminal residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 1 | |||||
Sequence: A |
Mass Spectrometry
2S albumin seed storage protein small subunit
Molecular mass is 4,640.1 Da. Determined by MALDI.2S albumin seed storage protein large subunit
Molecular mass is 8,440.72 Da. Determined by MALDI.2S albumin seed storage protein small subunit
Molecular mass is 3,437 Da. Determined by MALDI.2S albumin seed storage protein large subunit
Molecular mass is 8,009 Da. Determined by MALDI.Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U66866 EMBL· GenBank· DDBJ | AAB41308.1 EMBL· GenBank· DDBJ | mRNA |