P93198 · 2SS_JUGRE

Function

function

Seed storage protein.

Biotechnology

The recombinant protein could be used for a reliable component-resolved diagnosis of walnut allergy as it has the same properties and virtually identical IgE-binding reactivity as the native protein even that it is unprocessed and hence lacking the proteolytic cleavages of the mature protein (PubMed:19540419).
A rapid, highly specific and relatively sensitive indirect competitive enzyme-linked immunosorbent assay (ELISA) for detection and quantification of walnut protein component in processed foods has been developed using polyclonal antibodies against this protein (PubMed:24206692).
Testing young patients specific IgE-reactivity of this protein is a better approach in diagnosing clinical walnut allergy in children and youth compared to the walnut extract-based testing due to its improved specificity as shown by the receiver operating characteristic (ROC) statistical analysis (PubMed:28552380, PubMed:31175720).
The strategy of synthesizing digestion-resistant peptides instead of overlapping peptides, and verifying them using sera from walnut-allergic patients, can significantly reduce workload and serum requirement, enhance pertinence, and improve efficiency in studying linear epitopes of this protein (PubMed:32283362).

Temperature Dependence

Resistant to heat. Retains its alpha-helical fold after heating at 90 degrees Celsius and subsequent cooling to 20 degrees Celsius.

Features

Showing features for site.

TypeIDPosition(s)Description
Site113Necessary for maximum IgE-binding by the immunodominant epitope

GO annotations

AspectTerm
Cellular Componentextraorganismal space
Molecular Functionnutrient reservoir activity
Biological Processseed maturation

Keywords

Protein family/group databases

Names & Taxonomy

Protein names

Organism names

  • Taxonomic identifier
  • Strains
    • cv. Sunland
    • cv. Chandler
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fagales > Juglandaceae > Juglans

Accessions

  • Primary accession
    P93198

Proteomes

Genome annotation databases

Subcellular Location

Phenotypes & Variants

Allergenic properties

Causes an allergic reaction in human. Binds to IgE of patients allergic to walnuts (PubMed:11799381, PubMed:19540419, PubMed:22042002, PubMed:25388987, PubMed:27597723, PubMed:28552380, PubMed:31175720, PubMed:32283362, PubMed:9648708).
Binds to IgE of patients allergic to peanuts (PubMed:22042002).
Binds to IgE in 97% of the 32 Korean babies and children tested allergic to walnuts (PubMed:31175720).
Native protein binds to IgE in 100% of the 6 Chinese walnut-allergic patients tested (PubMed:32283362).
Native protein binds to IgE in 45% of the 11 walnut-allergic patients tested living in Spain or England (PubMed:25388987).
Recombinant protein binds to IgE in 75% of the 16 walnut-allergic patients tested (PubMed:9648708).
Recombinant protein binds to IgE in 100% of the 20 walnut-allergic patients tested (PubMed:11799381).
Recombinant protein binds to IgE in 55% and 61% of the 33 walnut-allergic adult patients living in Netherlands tested by ImmunoCAP and ImmunoCAP ISAC, respectively (PubMed:27597723).
Contains linear and conformational IgE-binding epitopes (PubMed:11799381).
Cross-reacts with sesame seed allergens Ses i 1 and Ses i 2. IgE-binding is lost in the presence of pepsin after 1 hour incubation at pH 1.5 and 37 degrees Celsius in vitro, but it is not affected in the presence of trypsin and chymotrypsin after 2 hour incubation in vitro at pH 8 and 37 degrees Celsius. Induces in vitro degranulation of the humanized rat basophilic leukemia (RBL) cells and release of beta-hexosaminidase from them (PubMed:19540419).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis104IgE-binding is retained by the immunodominant epitope.
Mutagenesis105IgE-binding is retained by the immunodominant epitope.
Mutagenesis106IgE-binding is retained by the immunodominant epitope.
Mutagenesis107Loss of IgE-binding by the immunodominant epitope.
Mutagenesis108Loss of IgE-binding by the immunodominant epitope.
Mutagenesis109Loss of IgE-binding by the immunodominant epitope.
Mutagenesis110Loss of IgE-binding by the immunodominant epitope.
Mutagenesis111IgE-binding is retained by the immunodominant epitope.
Mutagenesis112IgE-binding is retained by the immunodominant epitope.
Mutagenesis113IgE-binding is retained by the immunodominant epitope.
Mutagenesis114IgE-binding is retained by the immunodominant epitope.
Mutagenesis115IgE-binding is retained by the immunodominant epitope.

Keywords

Protein family/group databases

PTM/Processing

Features

Showing features for signal, propeptide, chain, disulfide bond.

TypeIDPosition(s)Description
Signal1-15
PropeptidePRO_000045111116-31
ChainPRO_000045111232-572S albumin seed storage protein small subunit
Disulfide bond39↔88Interchain (between small and large chains)
Disulfide bond52↔77Interchain (between small and large chains)
PropeptidePRO_000045111358-71
ChainPRO_000045111472-1352S albumin seed storage protein large subunit
Disulfide bond78↔125
Disulfide bond90↔132
PropeptidePRO_0000451115136-139

Keywords

Expression

Tissue specificity

Expressed in seed (at protein level) (PubMed:19540419, PubMed:25388987, PubMed:32283362).
Expressed in the peel of mature seed (PubMed:19540419).

Developmental stage

Expressed during seed maturation.

Interaction

Subunit

The mature protein consists of a small chain and a large chain linked by disulfide bonds.

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region16-30IgE-binding
Region29-34IgE-binding
Region64-78IgE-binding
Region65-73IgE-binding
Region95-103IgE-binding
Region99-111IgE-binding
Region102-114IgE-binding
Region104-115Immunodominant epitope. IgE-binding; binds to IgE in 75% of the 20 walnut-allergic patients tested
Region105-117IgE-binding
Region107-110Minimally required for IgE-binding by the immunodominant epitope
Region112-126IgE-binding
Region125-136IgE-binding
Region125-139IgE-binding

Sequence similarities

Belongs to the 2S seed storage albumins family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Fragment
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    139
  • Mass (Da)
    16,373
  • Last updated
    1997-05-01 v1
  • Checksum
    02D0E55E67164F23
AALLVALLFVANAAAFRTTITTMEIDEDIDNPRRRGEGCREQIQRQQNLNHCQYYLRQQSRSGGYDEDNQRQHFRQCCQQLSQMDEQCQCEGLRQVVRRQQQQQGLRGEEMEEMVQSARDLPNECGISSQRCEIRRSWF

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A2I4EX90A0A2I4EX90_JUGRELOC108993506146

Features

Showing features for non-terminal residue.

TypeIDPosition(s)Description
Non-terminal residue1

Mass Spectrometry

Molecular mass is 12,617.2 Da. Determined by MALDI. The measured mass is that of an intact non-reduced protein.

2S albumin seed storage protein small subunit

Molecular mass is 4,640.1 Da. Determined by MALDI.

2S albumin seed storage protein large subunit

Molecular mass is 8,440.72 Da. Determined by MALDI.

2S albumin seed storage protein small subunit

Molecular mass is 3,437 Da. Determined by MALDI.

2S albumin seed storage protein large subunit

Molecular mass is 8,009 Da. Determined by MALDI.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U66866
EMBL· GenBank· DDBJ
AAB41308.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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