P86938 · JBP1_TRYB2

Function

function

Dioxygenase that catalyzes the first step of DNA base J (beta-d-glucosyl-HOMedU) biosynthesis by converting thymine to 5-hydroxymethyluracil (HOMedU). DNA base J is a hypermodified thymidine residue found in the genome of kinetoplastid parasites, which is localized primarily to repetitive DNA, namely the telomeres, and is implicated in the regulation of antigenic variation. Also specifically binds to base J-containing DNA (J-DNA). Involved in propagation and maintenance of DNA base J synthesis initiated by JBP2 by specifically binding already synthesized DNA base J and propagating J synthesis. Thymine dioxygenase activity and J-DNA-binding are independent functions.

Catalytic activity

Cofactor

Fe2+ (UniProtKB | Rhea| CHEBI:29033 )

Note: Binds 1 Fe2+ ion per subunit.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site213Fe cation (UniProtKB | ChEBI); catalytic
Binding site215Fe cation (UniProtKB | ChEBI); catalytic
Binding site263Fe cation (UniProtKB | ChEBI); catalytic
Binding site2792-oxoglutarate (UniProtKB | ChEBI)
Site548Involved in J base recognition, conferring specificity towards J-DNA

GO annotations

AspectTerm
Cellular Componentnucleus
Molecular FunctionDNA binding
Molecular FunctionDNA translocase activity
Molecular Functionferrous iron binding
Molecular Functionthymine dioxygenase activity
Biological Processbase J metabolic process
Biological Processdouble-strand break repair via homologous recombination
Biological Processreciprocal meiotic recombination

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Thymine dioxygenase JBP1
  • EC number
  • Alternative names
    • J-binding protein 1
    • Thymidine hydroxylase JBP1

Gene names

    • Name
      JBP1
    • ORF names
      Tb11.01.5220

Organism names

Accessions

  • Primary accession
    P86938
  • Secondary accessions
    • Q382H3
    • Q9U6M3

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Does not affect growth, gene expression or the stability of some repetitive DNA sequences. The genome contains only about 5% of the wild-type level of J in their DNA. Cells lacking both JBP1 and JBP2 show a complete absence of base J.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis213Impairs DNA base J biosynthesis.
Mutagenesis215Impairs DNA base J biosynthesis.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003775551-839Thymine dioxygenase JBP1

Proteomic databases

Expression

Developmental stage

Expressed in bloodstream form and significantly less in procyclic form.

Interaction

Subunit

Monomer. Binds to DNA as a monomer (By similarity).

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region86-288Thymine dioxygenase
Region415-583DNA-binding JBP1 domain

Domain

The DNA-binding JBP1 domain (DB-JBP1) is necessary and sufficient for binding to J-DNA.

Sequence similarities

Belongs to the TET family. JBP1 subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    839
  • Mass (Da)
    95,251
  • Last updated
    2011-07-27 v1
  • Checksum
    B3BEECD851CC0FE7
MRRQVKKVLREKADDSMKPGWDVYQPSNDVVYAFNHYMQGSQIDAEAREKAEKAFQEAVKKHPFHNNADHTVDFHGTTVFRNAKGKVCGVLIPKALPSFATSMAADVLECAVARTSLRSALFGGVSPNSGIAGYFDYRGTPVELKCRKTSFTYEHTKEWRSVFPMIDYTSAIYKAALPDHWKAQDAAVPDVVRIHGSPFSTLTVNERFRTASHTDNGDFDNGYGVLAVLKGEYSGLSLALDDYGVCFNMQPTDVLLFDTHLFHSNTELEAKEANATWNRLSCVFYYRAALGEQPCVEEYRRRLKKAKEEKSTSLSFNHIEQKDNGENTNKPAPVYPVSLTPFSCAASAWALRGCAAAMLTRLHGLVRENASLMTELFGEPVEVADGLPRRAPEEIIPVHKHTNVQMHYLGGFSEKGDILNEAMNKRHYLDKENLQKMFGEEFVNIWTQSRTHWLQLVKKEWEHQKETNPTRTRFSWNNTSAMNFAFFDLCDVAKQLMCGAFGDREVNKKEEQSFWGMFAAHLDNACINEIGMLQGSMGMHKLNVKLKDYNFGGTRYLKDMPPEEQERRRRRRLEIEQARRRAPICDSESGDWLRNEAFDYQTEDVAVNYEREQWITPENNAKRFGFPERGVYGAEGAATGTISVLIVLPKPTNHRQKTCELPTSREADRIMKNPAAQRLLCAKPCNIGLSTSSNKSRTVLCGNIRIDKVFDGGSVGGKMYDFVIMRHLLAATTGEREPLECLVRWTSLARYCTFVVEVDLLDRHHYILKSEIGEEYSAVSEICFSALYSATYARDKVNLRTTPCLLSFIDKSGNMLESRFKFNGSPLNTVAFVVRRREK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CH464491
EMBL· GenBank· DDBJ
EAN80308.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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