P82896 · PA2A5_TRIST

Function

function

Snake venom phospholipase A2 (PLA2) that inhibits platelet aggregation induced by ADP, arachidonic acid and PAF (Ref.1). Acts in a enzymatic independent manner on a proteinase-activated receptor (PAR1, F2R) to evoke calcium release through the inositol 1,4,5-trisphosphate receptor (ITPR1, IP3R) and induces mouse aorta contraction (PubMed:25447533).
PAR1, phospholipase C and IP3R inhibitors suppress PA2-induced aorta contraction (PubMed:25447533).
PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides

Miscellaneous

Hemolytic and neurotoxic activities are not detected.

Catalytic activity

Cofactor

Ca2+ (UniProtKB | Rhea| CHEBI:29108 )

Note: Binds 1 Ca2+ ion.

Activity regulation

Preincubation with heparin slightly increase the enzymatic activity.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site27Ca2+ (UniProtKB | ChEBI)
Binding site29Ca2+ (UniProtKB | ChEBI)
Binding site31Ca2+ (UniProtKB | ChEBI)
Active site47
Binding site48Ca2+ (UniProtKB | ChEBI)
Active site89

GO annotations

AspectTerm
Cellular Componentextracellular region
Molecular Functioncalcium ion binding
Molecular Functioncalcium-dependent phospholipase A2 activity
Molecular Functionidentical protein binding
Molecular Functionphospholipid binding
Molecular Functiontoxin activity
Biological Processarachidonic acid secretion
Biological Processlipid catabolic process
Biological Processphospholipid metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Acidic phospholipase A2 5
  • EC number
  • Short names
    svPLA2
  • Alternative names
    • PA2-Vb
    • PLA2-V
    • Phosphatidylcholine 2-acylhydrolase

Organism names

Accessions

  • Primary accession
    P82896

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain, disulfide bond.

TypeIDPosition(s)Description
ChainPRO_00001617111-122Acidic phospholipase A2 5
Disulfide bond26↔115
Disulfide bond28↔44
Disulfide bond43↔95
Disulfide bond49↔122
Disulfide bond50↔88
Disulfide bond57↔81
Disulfide bond75↔86

Keywords

Expression

Tissue specificity

Expressed by the venom gland.

Interaction

Subunit

Monomer (predominant). Non-covalently linked homodimers are also observed.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P82896- P828962EBI-10105591, EBI-10105591

Protein-protein interaction databases

Family & Domains

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    122
  • Mass (Da)
    13,880
  • Last updated
    2001-03-01 v1
  • Checksum
    8B20A1D3907EB522
NLMQFELLIMKVAGRSGIVWYSDYGCFCGKGGHGRPQDATDRCCFVHDCCYGKVTECDPKMDFYRYSSNNGDIVCEANNPCTKEICECDKAAAICFRDNKDTYDNKYWNIPMEGCQEESEPC

Keywords

Similar Proteins

Disclaimer

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