P55036 · PSMD4_HUMAN

  • Protein
    26S proteasome non-ATPase regulatory subunit 4
  • Gene
    PSMD4
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMD4 acts as an ubiquitin receptor subunit through ubiquitin-interacting motifs and selects ubiquitin-conjugates for destruction. Displays a preferred selectivity for longer polyubiquitin chains.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytosol
Cellular Componentnucleoplasm
Cellular Componentnucleus
Cellular Componentproteasome accessory complex
Cellular Componentproteasome complex
Cellular Componentproteasome regulatory particle, base subcomplex
Molecular Functionidentical protein binding
Molecular Functionmolecular adaptor activity
Molecular Functionpolyubiquitin modification-dependent protein binding
Molecular FunctionRNA binding
Biological Processproteasome-mediated ubiquitin-dependent protein catabolic process

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    26S proteasome non-ATPase regulatory subunit 4
  • Alternative names
    • 26S proteasome regulatory subunit RPN10
    • 26S proteasome regulatory subunit S5A
    • Antisecretory factor 1 (AF; ASF)
    • Multiubiquitin chain-binding protein

Gene names

    • Name
      PSMD4
    • Synonyms
      MCB1

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo

Accessions

  • Primary accession
    P55036
  • Secondary accessions
    • D3DV16
    • Q5VWC5
    • Q9NS92

Proteomes

Organism-specific databases

Disease & Variants

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 352 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

Organism-specific databases

Miscellaneous

Chemistry

Genetic variation databases

PTM/Processing

Features

Showing features for chain, cross-link, modified residue, modified residue (large scale data).

TypeIDPosition(s)SourceDescription
ChainPRO_00001738281-377UniProt26S proteasome non-ATPase regulatory subunit 4
Cross-link122UniProtGlycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)
Modified residue250UniProtPhosphothreonine
Modified residue253UniProtPhosphothreonine
Modified residue256UniProtPhosphoserine
Modified residue (large scale data)256PRIDEPhosphoserine
Modified residue266UniProtPhosphoserine
Modified residue (large scale data)266PRIDEPhosphoserine
Modified residue358UniProtPhosphoserine
Modified residue (large scale data)358PRIDEPhosphoserine
Modified residue361UniProtPhosphoserine
Modified residue (large scale data)361PRIDEPhosphoserine

Keywords

Proteomic databases

PTM databases

Expression

Gene expression databases

Organism-specific databases

Interaction

Subunit

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases and few additional components including PSMD4 (PubMed:27342858, PubMed:27428775).
Interacts with NUB1 (PubMed:11585840).
Interacts with SQSTM1 (PubMed:15340068).
Interacts with UBQLN4 (PubMed:15280365).
Interacts with UBE3A (PubMed:22645313).
Interacts with UBQLN1 (via ubiquitin-like domain) (PubMed:15147878).
Interacts with DDI2 (PubMed:29290612).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P55036ADRM1 Q161866EBI-359318, EBI-954387
BINARY P55036CFTR P1356913EBI-359318, EBI-349854
BINARY P55036HTT P428585EBI-359318, EBI-466029
XENO P55036Pax3 P246103EBI-359318, EBI-1208116
BINARY P55036PSMA2 P257872EBI-359318, EBI-603262
BINARY P55036PSMA5 P280663EBI-359318, EBI-355475
BINARY P55036PSMA7 O148182EBI-359318, EBI-603272
BINARY P55036PSMB1 P206183EBI-359318, EBI-372273
BINARY P55036PSMB7 Q994363EBI-359318, EBI-603319
BINARY P55036PSMC1 P621915EBI-359318, EBI-357598
BINARY P55036PSMC2 P359985EBI-359318, EBI-359710
BINARY P55036PSMC3 P179803EBI-359318, EBI-359720
BINARY P55036PSMC4 P436864EBI-359318, EBI-743997
BINARY P55036PSMC5 P621955EBI-359318, EBI-357745
BINARY P55036PSMC6 P623335EBI-359318, EBI-357669
BINARY P55036PSMD1 Q994604EBI-359318, EBI-357874
BINARY P55036PSMD11 O002315EBI-359318, EBI-357816
BINARY P55036PSMD12 O002325EBI-359318, EBI-359733
BINARY P55036PSMD13 Q9UNM64EBI-359318, EBI-356070
BINARY P55036PSMD2 Q132007EBI-359318, EBI-357648
BINARY P55036PSMD3 O432425EBI-359318, EBI-357622
BINARY P55036PSMD4 P550363EBI-359318, EBI-359318
BINARY P55036PSMD6 Q150084EBI-359318, EBI-359701
BINARY P55036PSMD8 P485562EBI-359318, EBI-359304
BINARY P55036RAD23A P5472516EBI-359318, EBI-746453
BINARY P55036RAD23B P5472711EBI-359318, EBI-954531
XENO P55036Rbck1 Q629213EBI-359318, EBI-7266339
BINARY P55036SCHIP1 P0DPB32EBI-359318, EBI-1397509
BINARY P55036SHANK3 Q9BYB02EBI-359318, EBI-1752330
BINARY P55036TCP11L1 Q9NUJ35EBI-359318, EBI-2555179
BINARY P55036UBC P0CG484EBI-359318, EBI-3390054
BINARY P55036UBE3A Q050865EBI-359318, EBI-954357
BINARY P55036UBE3A Q05086-23EBI-359318, EBI-10175863
BINARY P55036UBQLN1 Q9UMX04EBI-359318, EBI-741480
BINARY P55036UCHL5 Q9Y5K58EBI-359318, EBI-1051183

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain5-188VWFA
Region197-262Interaction with UBQLN1
Domain211-230UIM 1
Region216-220Essential for ubiquitin-binding
Region224-255Disordered
Compositional bias225-239Basic and acidic residues
Domain282-301UIM 2
Region287-291Essential for ubiquitin-binding
Region300-327Disordered
Region341-377Disordered

Domain

The 2 UIM motifs are involved in the binding to a multi-ubiquitin chain in a cooperative way.

Sequence similarities

Belongs to the proteasome subunit S5A family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing. Additional isoforms seem to exist.

P55036-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Name
    Rpn10A
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Length
    377
  • Mass (Da)
    40,737
  • Last updated
    1996-10-01 v1
  • Checksum
    EC712EC4DB1CE9AB
MVLESTMVCVDNSEYMRNGDFLPTRLQAQQDAVNIVCHSKTRSNPENNVGLITLANDCEVLTTLTPDTGRILSKLHTVQPKGKITFCTGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDNEKDLVKLAKRLKKEKVNVDIINFGEEEVNTEKLTAFVNTLNGKDGTGSHLVTVPPGPSLADALISSPILAGEGGAMLGLGASDFEFGVDPSADPELALALRVSMEEQRQRQEEEARRAAAASAAEAGIATTGTEDSDDALLKMTISQQEFGRTGLPDLSSMTEEEQIAYAMQMSLQGAEFGQAESADIDASSAMDTSEPAKEEDDYDVMQDPEFLQSVLENLPGVDPNNEAIRNAMGSLASQATKDGKKDKKEEDKK

P55036-2

  • Name
    Rpn10E
  • Note
    May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Computationally mapped potential isoform sequences

There are 5 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
H0Y3Y9H0Y3Y9_HUMANPSMD4193
H0Y561H0Y561_HUMANPSMD484
Q5VWC4Q5VWC4_HUMANPSMD4380
H7C2J9H7C2J9_HUMANPSMD439
A6PVX3A6PVX3_HUMANPSMD4203

Features

Showing features for compositional bias, alternative sequence.

TypeIDPosition(s)Description
Compositional bias225-239Basic and acidic residues
Alternative sequenceVSP_005291255-268in isoform Rpn10E
Alternative sequenceVSP_005292269-377in isoform Rpn10E

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U51007
EMBL· GenBank· DDBJ
AAC50433.1
EMBL· GenBank· DDBJ
mRNA
U24704
EMBL· GenBank· DDBJ
AAB54057.1
EMBL· GenBank· DDBJ
mRNA
AB033605
EMBL· GenBank· DDBJ
BAA97581.1
EMBL· GenBank· DDBJ
mRNA
AL391069
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
AL592424
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
CH471121
EMBL· GenBank· DDBJ
EAW53457.1
EMBL· GenBank· DDBJ
Genomic DNA
CH471121
EMBL· GenBank· DDBJ
EAW53458.1
EMBL· GenBank· DDBJ
Genomic DNA
BC002365
EMBL· GenBank· DDBJ
AAH02365.1
EMBL· GenBank· DDBJ
mRNA
BC072008
EMBL· GenBank· DDBJ
AAH72008.1
EMBL· GenBank· DDBJ
mRNA
U72664
EMBL· GenBank· DDBJ
AAB68598.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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