P47959 · HPRT_MERUN
- ProteinHypoxanthine-guanine phosphoribosyltransferase
- GeneHPRT1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids218 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway (By similarity).
Catalytic activity
- IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphateThis reaction proceeds in the backward direction.
Cofactor
Note: Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein.
Pathway
Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 69 | GMP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 134-142 | GMP (UniProtKB | ChEBI) | ||||
Sequence: EDIIDTGKT | ||||||
Active site | 138 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 166 | GMP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 186-188 | GMP (UniProtKB | ChEBI) | ||||
Sequence: KFV | ||||||
Binding site | 194 | GMP (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 194 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | cytosol | |
Molecular Function | guanine phosphoribosyltransferase activity | |
Molecular Function | hypoxanthine phosphoribosyltransferase activity | |
Molecular Function | identical protein binding | |
Molecular Function | magnesium ion binding | |
Molecular Function | nucleotide binding | |
Biological Process | GMP catabolic process | |
Biological Process | GMP salvage | |
Biological Process | guanine salvage | |
Biological Process | hypoxanthine metabolic process | |
Biological Process | hypoxanthine salvage | |
Biological Process | IMP metabolic process | |
Biological Process | IMP salvage | |
Biological Process | positive regulation of dopamine metabolic process | |
Biological Process | purine nucleotide biosynthetic process | |
Biological Process | purine ribonucleoside salvage |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameHypoxanthine-guanine phosphoribosyltransferase
- EC number
- Short namesHGPRT; HGPRTase
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Gerbillinae > Meriones
Accessions
- Primary accessionP47959
Subcellular Location
PTM/Processing
Features
Showing features for initiator methionine, modified residue, chain, cross-link.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Modified residue | 2 | N-acetylalanine | ||||
Sequence: A | ||||||
Chain | PRO_0000139587 | 2-218 | Hypoxanthine-guanine phosphoribosyltransferase | |||
Sequence: ATRSPSIVIGDDEPGYDLDLFCIPKHYAEDLEKVFIPHGLIMDRTERLARDVMKEMGGHHIVALCVLKGGYKFFADLLDYIKSLNRNTDRSIPMTVDFIRLKSYCNDQSTGDIKVIGGDDLSALTGKNVLIVEDIIDTGKTMQTLLSLVQKYSPKMVKVASLLVKRTPRSVGYRPDFVGFEIPDKFVVGYALDYNEYFRDLNHVCVISESGKAKYKA | ||||||
Modified residue | 103 | N6-acetyllysine | ||||
Sequence: K | ||||||
Cross-link | 115 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternate | ||||
Sequence: K | ||||||
Cross-link | 115 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate | ||||
Sequence: K | ||||||
Modified residue | 142 | Phosphothreonine | ||||
Sequence: T |
Keywords
- PTM
Interaction
Subunit
Homotetramer.
Structure
Sequence
- Sequence statusComplete
- Length218
- Mass (Da)24,538
- Last updated2007-01-23 v2
- Checksum589C500AD78CE9FC