P47953 · LEG3_CRILO

Function

function

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity).
In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes (By similarity).
When secreted, interacts with NK cell-activating receptor NCR3/NKp30 acting as an inhibitory ligand which antagonizes NK cell attack (By similarity).

Features

Showing features for binding site.

124520406080100120140160180200220240
TypeIDPosition(s)Description
Binding site176-182a beta-D-galactoside (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcollagen-containing extracellular matrix
Cellular Componentcytoplasm
Cellular Componentextracellular space
Cellular Componentimmunological synapse
Cellular Componentspliceosomal complex
Molecular Functioncarbohydrate binding
Molecular FunctionIgE binding
Molecular Functionlaminin binding
Biological Processcell differentiation
Biological Processeosinophil chemotaxis
Biological Processinnate immune response
Biological Processmacrophage chemotaxis
Biological Processmonocyte chemotaxis
Biological ProcessmRNA processing
Biological Processnegative regulation of endocytosis
Biological Processnegative regulation of extrinsic apoptotic signaling pathway
Biological Processneutrophil chemotaxis
Biological Processpositive chemotaxis
Biological Processpositive regulation of calcium ion import
Biological ProcessRNA splicing

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Galectin-3
  • Short names
    Gal-3
  • Alternative names
    • 35 kDa lectin
    • CBP30
    • Carbohydrate-binding protein 35 (CBP 35)
    • Galactose-specific lectin 3
    • IgE-binding protein

Gene names

    • Name
      LGALS3

Organism names

Accessions

  • Primary accession
    P47953

Subcellular Location

Cytoplasm
Nucleus
Secreted
Note: Secreted by a non-classical secretory pathway and associates with the cell surface. Can be secreted; the secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10; it results in protein translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) followed by vesicle entry and secretion.

Keywords

PTM/Processing

Features

Showing features for initiator methionine, modified residue, chain, disulfide bond.

TypeIDPosition(s)Description
Initiator methionine1Removed
Modified residue2N-acetylalanine
ChainPRO_00000769292-245Galectin-3
Modified residue6Phosphoserine; by CK1
Disulfide bond168Interchain
Modified residue183Phosphoserine

Keywords

Interaction

Subunit

Probably forms homo- or heterodimers. Interacts with DMBT1 (By similarity).
Interacts with CD6 and ALCAM. Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, ZFTRAF1 and UACA. Interacts with TRIM16; this interaction mediates autophagy of damage endomembranes. Interacts with cargo receptor TMED10; the interaction mediates the translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and thereby secretion (By similarity).
Interacts with and inhibits by binding NCR3/NKp30 (By similarity).

Structure

Family & Domains

Features

Showing features for region, repeat, domain, motif.

TypeIDPosition(s)Description
Region1-30Disordered
Repeat35-431
Region35-997 X 9 AA tandem repeats of Y-P-G-X3-P-[GS]-A
Repeat44-522
Region47-68Disordered
Repeat53-613
Repeat62-704
Repeat71-785; approximate
Repeat79-886; approximate
Repeat89-997; approximate
Domain113-243Galectin
Motif221-236Nuclear export signal

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    245
  • Mass (Da)
    25,739
  • Last updated
    2007-01-23 v2
  • Checksum
    9469B9AA146EEF76
MADGFSLNDALAGSGNPNPQGWPGAWGNQPGAGGYPGASYPGAYPGQAPPGAYPGQAPPGAYPGPTAPGAYPGPAPGAYPGQPGASGAYPSAPGAYPAAGPYGAPTGALTVPYKLPLAGGVMPRMLITIMGTVKPNANRIILNFLRGNDIAFHFNPRFNENNRRVIVCNTKQDNNWGREERQSAFPFESGRPFKIQVLVEADHFKVAVNDAHLLQYNHRMKNLREINQMEISGDITLTSAAPTMI

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X78879
EMBL· GenBank· DDBJ
CAA55479.1
EMBL· GenBank· DDBJ
mRNA

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