P47953 · LEG3_CRILO
- ProteinGalectin-3
- GeneLGALS3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids245 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity).
In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes (By similarity).
When secreted, interacts with NK cell-activating receptor NCR3/NKp30 acting as an inhibitory ligand which antagonizes NK cell attack (By similarity).
In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes (By similarity).
When secreted, interacts with NK cell-activating receptor NCR3/NKp30 acting as an inhibitory ligand which antagonizes NK cell attack (By similarity).
Features
Showing features for binding site.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | collagen-containing extracellular matrix | |
Cellular Component | cytoplasm | |
Cellular Component | extracellular space | |
Cellular Component | immunological synapse | |
Cellular Component | spliceosomal complex | |
Molecular Function | carbohydrate binding | |
Molecular Function | IgE binding | |
Molecular Function | laminin binding | |
Biological Process | cell differentiation | |
Biological Process | eosinophil chemotaxis | |
Biological Process | innate immune response | |
Biological Process | macrophage chemotaxis | |
Biological Process | monocyte chemotaxis | |
Biological Process | mRNA processing | |
Biological Process | negative regulation of endocytosis | |
Biological Process | negative regulation of extrinsic apoptotic signaling pathway | |
Biological Process | neutrophil chemotaxis | |
Biological Process | positive chemotaxis | |
Biological Process | positive regulation of calcium ion import | |
Biological Process | RNA splicing |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameGalectin-3
- Short namesGal-3
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Cricetidae > Cricetinae > Cricetulus
Accessions
- Primary accessionP47953
Subcellular Location
UniProt Annotation
GO Annotation
Note: Secreted by a non-classical secretory pathway and associates with the cell surface. Can be secreted; the secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10; it results in protein translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) followed by vesicle entry and secretion.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for initiator methionine, modified residue, chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Modified residue | 2 | N-acetylalanine | ||||
Sequence: A | ||||||
Chain | PRO_0000076929 | 2-245 | Galectin-3 | |||
Sequence: ADGFSLNDALAGSGNPNPQGWPGAWGNQPGAGGYPGASYPGAYPGQAPPGAYPGQAPPGAYPGPTAPGAYPGPAPGAYPGQPGASGAYPSAPGAYPAAGPYGAPTGALTVPYKLPLAGGVMPRMLITIMGTVKPNANRIILNFLRGNDIAFHFNPRFNENNRRVIVCNTKQDNNWGREERQSAFPFESGRPFKIQVLVEADHFKVAVNDAHLLQYNHRMKNLREINQMEISGDITLTSAAPTMI | ||||||
Modified residue | 6 | Phosphoserine; by CK1 | ||||
Sequence: S | ||||||
Disulfide bond | 168 | Interchain | ||||
Sequence: C | ||||||
Modified residue | 183 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Interaction
Subunit
Probably forms homo- or heterodimers. Interacts with DMBT1 (By similarity).
Interacts with CD6 and ALCAM. Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, ZFTRAF1 and UACA. Interacts with TRIM16; this interaction mediates autophagy of damage endomembranes. Interacts with cargo receptor TMED10; the interaction mediates the translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and thereby secretion (By similarity).
Interacts with and inhibits by binding NCR3/NKp30 (By similarity).
Interacts with CD6 and ALCAM. Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, ZFTRAF1 and UACA. Interacts with TRIM16; this interaction mediates autophagy of damage endomembranes. Interacts with cargo receptor TMED10; the interaction mediates the translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and thereby secretion (By similarity).
Interacts with and inhibits by binding NCR3/NKp30 (By similarity).
Structure
Family & Domains
Features
Showing features for region, repeat, domain, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-30 | Disordered | ||||
Sequence: MADGFSLNDALAGSGNPNPQGWPGAWGNQP | ||||||
Repeat | 35-43 | 1 | ||||
Sequence: YPGASYPGA | ||||||
Region | 35-99 | 7 X 9 AA tandem repeats of Y-P-G-X3-P-[GS]-A | ||||
Sequence: YPGASYPGAYPGQAPPGAYPGQAPPGAYPGPTAPGAYPGPAPGAYPGQPGASGAYPSAPGAYPAA | ||||||
Repeat | 44-52 | 2 | ||||
Sequence: YPGQAPPGA | ||||||
Region | 47-68 | Disordered | ||||
Sequence: QAPPGAYPGQAPPGAYPGPTAP | ||||||
Repeat | 53-61 | 3 | ||||
Sequence: YPGQAPPGA | ||||||
Repeat | 62-70 | 4 | ||||
Sequence: YPGPTAPGA | ||||||
Repeat | 71-78 | 5; approximate | ||||
Sequence: YPGPAPGA | ||||||
Repeat | 79-88 | 6; approximate | ||||
Sequence: YPGQPGASGA | ||||||
Repeat | 89-99 | 7; approximate | ||||
Sequence: YPSAPGAYPAA | ||||||
Domain | 113-243 | Galectin | ||||
Sequence: YKLPLAGGVMPRMLITIMGTVKPNANRIILNFLRGNDIAFHFNPRFNENNRRVIVCNTKQDNNWGREERQSAFPFESGRPFKIQVLVEADHFKVAVNDAHLLQYNHRMKNLREINQMEISGDITLTSAAPT | ||||||
Motif | 221-236 | Nuclear export signal | ||||
Sequence: KNLREINQMEISGDIT |
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length245
- Mass (Da)25,739
- Last updated2007-01-23 v2
- Checksum9469B9AA146EEF76