P35852 · PURL_LACCA

Function

function

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.

Catalytic activity

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide: step 1/2.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site55
Binding site58ATP (UniProtKB | ChEBI)
Binding site97ATP (UniProtKB | ChEBI)
Binding site99Mg2+ 1 (UniProtKB | ChEBI)
Binding site100-103substrate
Active site101Proton acceptor
Binding site122substrate
Binding site123Mg2+ 2 (UniProtKB | ChEBI)
Binding site246substrate
Binding site276Mg2+ 2 (UniProtKB | ChEBI)
Binding site320-322substrate
Binding site501ATP (UniProtKB | ChEBI)
Binding site538ATP (UniProtKB | ChEBI)
Binding site539Mg2+ 1 (UniProtKB | ChEBI)
Binding site541substrate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionmagnesium ion binding
Molecular Functionphosphoribosylformylglycinamidine synthase activity
Biological Process'de novo' IMP biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Phosphoribosylformylglycinamidine synthase subunit PurL
  • EC number
  • Short names
    FGAM synthase
  • Alternative names
    • Formylglycinamide ribonucleotide amidotransferase subunit II
      (FGAR amidotransferase II
      ; FGAR-AT II
      )
    • Glutamine amidotransferase PurL
    • Phosphoribosylformylglycinamidine synthase subunit II

Gene names

    • Name
      purL

Organism names

Accessions

  • Primary accession
    P35852

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001004601-740Phosphoribosylformylglycinamidine synthase subunit PurL

Interaction

Subunit

Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the FGAMS family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    740
  • Mass (Da)
    79,481
  • Last updated
    1996-02-01 v2
  • Checksum
    03238AB3CD1C8FF5
MYVVHVEMSPEAIATQKPYLDLGLTEAEYDRFAELIGHQPNDTEIGLASGMWSEHCAYKYSKPVLRQFWTKNERVLMGPGEGAGVIDIGEGKAVVFKAESHNHPSAVEPYEGAATGVGGIIRDIFSIGAKPVAMLDSLAFGDIEQPHTQHLVDRIVAGIGGYGNAIGIPTVGGETNFDGSYTRNPLVNAMCVGIMDKDQIQKGKAAGVGNALIYVGAKTGRDGINGASFASGDFSDEEAADRSAVQVGDPFMEKLLMDACLEITGHHQEALVGIQDMGAAGLVSSSVEMAGKANSGMVLDLDLIPQREAEMTPFEIMLSESQERMLLCVRAGFEQEVLAVFADYDLDAAIVGHVIAGHQYQLYHHGKLVCDVPVSSLTDDAPIYHQQGKMPKRLAQPAADFDPIITDPVQIWTDMMAMPTIADKSSLYKRYDAQVQTNTVVLPGSDAAVIRIRGTHRALAMTTDSKDVTCILIAGGCGNECWLKRARNLVASGAEPLGITDCLNFGDPTKPEAFYELAEAAKGIIAATKAFNAPVISGNVSLYNETNGEAIYPTPMIGMVGLIEDLSTITTAAFKQADDLIYLVGETHGDFNGSELQKLQTGEVTGKLFDFDLEAEKQHQHFVLKAIREHLITAAHDLSDGGLLVALAEMGFDAQLGAQINVTLPTAWGFSETQGRFLLTVSPENQAAFEALHGPAQLIGRVQAPPEFEVTTVNQHFSASLQQLQTAFEEALPCHMNQKA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M85265
EMBL· GenBank· DDBJ
AAC36947.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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