P31434 · XYLS_ECOLI

Function

function

Can catalyze the transfer of alpha-xylosyl residue from alpha-xyloside to xylose, glucose, mannose, fructose, maltose, isomaltose, nigerose, kojibiose, sucrose and trehalose.

Catalytic activity

  • Hydrolysis of terminal, non-reducing alpha-D-xylose residues with release of alpha-D-xylose.
    EC:3.2.1.177 (UniProtKB | ENZYME | Rhea)

pH Dependence

Optimum pH is 7.0.

Features

Showing features for active site.

1772100200300400500600700
TypeIDPosition(s)Description
Active site416Nucleophile
Active site419
Active site482Proton donor

GO annotations

AspectTerm
Molecular Functionalpha-D-xyloside xylohydrolase
Molecular Functioncarbohydrate binding
Molecular Functionidentical protein binding
Molecular Functionxyloglucan 1,6-alpha-xylosidase activity
Biological Processcarbohydrate metabolic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

Gene names

    • Name
      yicI
    • Ordered locus names
      b3656, JW3631

Organism names

  • Taxonomic identifier
  • Strains
    • K12 / MG1655 / ATCC 47076
    • K12 / W3110 / ATCC 27325 / DSM 5911
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    P31434
  • Secondary accessions
    • P76723
    • Q2M7W9

Proteomes

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis307-308Converts the enzyme to have alpha-glucosidase activity.

Chemistry

    • DB03586(3R,4R,5S,6R)-6-Fluoro-3,4,5-trihydroxytetrahydro-2H-pyran-2-olate
    • DB038142-(N-morpholino)ethanesulfonic acid
    • DB034343-(N-morpholino)propanesulfonic acid
    • DB048074-NITROPHENYL-(6-S-ALPHA-D-XYLOPYRANOSYL)-BETA-D-GLUCOPYRANOSIDE

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001853711-772Alpha-xylosidase

Proteomic databases

Interaction

Subunit

Homohexamer.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the glycosyl hydrolase 31 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    772
  • Mass (Da)
    88,079
  • Last updated
    1998-07-15 v2
  • Checksum
    6F2A02E4B5403772
MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRERTWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAEFKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGETVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSFEVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTSFTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRRLKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAGDVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRNVVKVNGLQDGSQAESEQGLVVKPQGNALTITL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
L10328
EMBL· GenBank· DDBJ
AAA62009.1
EMBL· GenBank· DDBJ
Genomic DNA
U00096
EMBL· GenBank· DDBJ
AAC76680.1
EMBL· GenBank· DDBJ
Genomic DNA
AP009048
EMBL· GenBank· DDBJ
BAE77637.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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