P24618 · GRM_MICEC
- Protein16S rRNA (guanine(1405)-N(7))-methyltransferase
- Genegrm
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids274 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Specifically methylates the N7 position of guanine 1405 in 16S rRNA. Confers resistance to various aminoglycosides, including gentamicin and kanamycin.
Miscellaneous
Protects M.echinospora, which is an antibiotic-producing bacterium, against self-intoxication.
Catalytic activity
- guanosine1405 in 16S rRNA + S-adenosyl-L-methionine = N7-methylguanosine1405 in 16S rRNA + S-adenosyl-L-homocysteine
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 64 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: F | ||||||
Binding site | 102-104 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: HVS | ||||||
Binding site | 108 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 133 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: A | ||||||
Binding site | 156 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 182-183 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: DL | ||||||
Binding site | 198 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Binding site | 207 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: Q |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | rRNA methyltransferase activity | |
Biological Process | response to antibiotic |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name16S rRNA (guanine(1405)-N(7))-methyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Micromonosporales > Micromonosporaceae > Micromonospora
Accessions
- Primary accessionP24618
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000083854 | 1-274 | 16S rRNA (guanine1405-N7)-methyltransferase | |||
Sequence: MTTSAPEDRIDQVEQAITKSRRYQTVAPATVRRLARAALVAARGDVPDAVKRTKRGLHEIYGAFLPPSPPNYAALLRQLDSAVDAGDDEAVRAALRRAMSVHVSTRERLPHLAEFYQEIFRHVPQPNTLRDLACGLNPLAAPWMGLSDQTVYVASDIDARLIGFVDAALTRLGVAHRTSVVDLLEDRLDEPTDVTLLLKTLPCLETQRRGSGWEVIDIVNSPIIVVTFPTKSLGQRSKGMFQNYSQSFESQARERSCRIQRLEIGNELIYVIQK |
Structure
Family & Domains
Sequence similarities
Belongs to the methyltransferase superfamily. Aminoglycoside resistance family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length274
- Mass (Da)30,542
- Last updated1992-03-01 v1
- Checksum9D7289559621A68E
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M55520 EMBL· GenBank· DDBJ | AAA25336.1 EMBL· GenBank· DDBJ | Genomic DNA |