P21505 · DMO1_DESMO
- ProteinHoming endonuclease I-DmoI
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids194 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Endonuclease involved in intron homing (Probable). Recognizes DNA in the 23S rRNA gene intron (minimally 5'-CCGGGTAAGTTCCGG-3'), cutting after A-8 on the top and C-11 on the bottom strand. Has a slow turnover rate, cuts the coding strand with a slight preference over the non-coding strand (PubMed:9092657).
Miscellaneous
Translated from a circularized intron.
Cofactor
Note: Mn2+, Co2+ and Mg2+ enable equal cleavage, Zn2+ is less active and Ni2+ is essentially inactive.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
4 nM | a 25-bp DNA substrate |
Temperature Dependence
Not extremely thermostable, has lost significant activity after 90 minues at 65 degreees Celsius, has no activity after 4 hours.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 21 | |||||
Sequence: D | ||||||
Active site | 117 | |||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | endonuclease activity | |
Biological Process | intein-mediated protein splicing | |
Biological Process | intron homing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameHoming endonuclease I-DmoI
- EC number
Organism names
- Taxonomic lineageArchaea > Thermoproteota > Thermoprotei > Desulfurococcales > Desulfurococcaceae > Desulfurococcus
Accessions
- Primary accessionP21505
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000192791 | 1-194 | Homing endonuclease I-DmoI | |||
Sequence: MHNNENVSGISAYLLGLIIGDGGLYKLKYKGNRSEYRVVITQKSENLIKQHIAPLMQFLIDELNVKSKIQIVKGDTRYELRVSSKKLYYYFANMLERIRLFNMREQIAFIKGLYVAEGDKTLKRLRIWNKNKALLEIVSRWLNNLGVRNTIHLDDHRHGVYVLNISLRDRIKFVHTILSSHLNPLPPERAGGYT |
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 14-147 | DOD-type homing endonuclease | ||||
Sequence: LLGLIIGDGGLYKLKYKGNRSEYRVVITQKSENLIKQHIAPLMQFLIDELNVKSKIQIVKGDTRYELRVSSKKLYYYFANMLERIRLFNMREQIAFIKGLYVAEGDKTLKRLRIWNKNKALLEIVSRWLNNLGV |
Family and domain databases
Sequence
- Sequence statusComplete
- Length194
- Mass (Da)22,609
- Last updated1991-05-01 v1
- Checksum99ED3354907F35A3
Keywords
- Technical term