P21505 · DMO1_DESMO

Function

function

Endonuclease involved in intron homing (Probable). Recognizes DNA in the 23S rRNA gene intron (minimally 5'-CCGGGTAAGTTCCGG-3'), cutting after A-8 on the top and C-11 on the bottom strand. Has a slow turnover rate, cuts the coding strand with a slight preference over the non-coding strand (PubMed:9092657).

Miscellaneous

Translated from a circularized intron.

Cofactor

a divalent metal cation (UniProtKB | Rhea| CHEBI:60240 )

Note: Mn2+, Co2+ and Mg2+ enable equal cleavage, Zn2+ is less active and Ni2+ is essentially inactive.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
4 nMa 25-bp DNA substrate

Temperature Dependence

Not extremely thermostable, has lost significant activity after 90 minues at 65 degreees Celsius, has no activity after 4 hours.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site21
Active site117

GO annotations

AspectTerm
Molecular Functionendonuclease activity
Biological Processintein-mediated protein splicing
Biological Processintron homing

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Homing endonuclease I-DmoI
  • EC number

Organism names

Accessions

  • Primary accession
    P21505

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001927911-194Homing endonuclease I-DmoI

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain14-147DOD-type homing endonuclease

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    194
  • Mass (Da)
    22,609
  • Last updated
    1991-05-01 v1
  • Checksum
    99ED3354907F35A3
MHNNENVSGISAYLLGLIIGDGGLYKLKYKGNRSEYRVVITQKSENLIKQHIAPLMQFLIDELNVKSKIQIVKGDTRYELRVSSKKLYYYFANMLERIRLFNMREQIAFIKGLYVAEGDKTLKRLRIWNKNKALLEIVSRWLNNLGVRNTIHLDDHRHGVYVLNISLRDRIKFVHTILSSHLNPLPPERAGGYT

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X03263
EMBL· GenBank· DDBJ
CAA27018.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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