P16153 · TCDE_CLODI
- ProteinHolin-like protein TcdE
- GenetcdE
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids166 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Isoform 1
Holin-like protein required for secretion of toxins A and B (TcdA and TcdB) (PubMed:22685398).
Facilitates the release of toxins to the extracellular environment without causing the bacterial cell lysis (PubMed:22685398).
Facilitates the release of toxins to the extracellular environment without causing the bacterial cell lysis (PubMed:22685398).
Isoform 2
Has weak activity, suggesting that it may act as a antiholin when multiple forms are produced.
Isoform 3
Has weak activity, suggesting that it may act as a antiholin when multiple forms are produced.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | pore-forming activity | |
Biological Process | protein transport |
Keywords
- Biological process
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameHolin-like protein TcdE
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Clostridia > Peptostreptococcales > Peptostreptococcaceae > Clostridioides
Accessions
- Primary accessionP16153
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 15-35 | Helical | ||||
Sequence: IFFYINLGGVMNMTISFLSEH | ||||||
Transmembrane | 36-56 | Helical | ||||
Sequence: IFIKLVILTISFDTLLGCLSA | ||||||
Transmembrane | 77-97 | Helical | ||||
Sequence: MIACIFFLSVVDILTKFNFLF | ||||||
Transmembrane | 111-131 | Helical | ||||
Sequence: HLGISEFFSILFILYESVSIL |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Cells grow normally but accumulate a strongly reduced amount of toxins TcdA and TcdB in the medium.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000022643 | 1-166 | Holin-like protein TcdE | |||
Sequence: MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILTISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQDCINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEMNAKDEK |
Interaction
Subunit
Homomultimer.
Structure
Family & Domains
Sequence similarities
Belongs to the bacteriophage holin family.
Keywords
- Domain
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 3 isoforms produced by Alternative initiation.
P16153-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- SynonymsLong
- Length166
- Mass (Da)18,800
- Last updated1996-10-01 v2
- Checksum1671FAE1884C7EE2
P16153-2
- Name2
- SynonymsShort
- Differences from canonical
- 1-26: Missing
P16153-3
- Name3
- Differences from canonical
- 1-24: Missing
Features
Showing features for alternative sequence.
Keywords
- Coding sequence diversity
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X60984 EMBL· GenBank· DDBJ | CAA43300.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X60984 EMBL· GenBank· DDBJ | CAA43301.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M30308 EMBL· GenBank· DDBJ | AAA83532.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M30308 EMBL· GenBank· DDBJ | AAA83533.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X92982 EMBL· GenBank· DDBJ | CAA63563.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X51797 EMBL· GenBank· DDBJ | CAA36093.1 EMBL· GenBank· DDBJ | Genomic DNA |