P00627 · PA2B6_BUNFA
- ProteinBasic phospholipase A2 6
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids135 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Snake venom phospholipase A2 (PLA2) that inhibits neuromuscular transmission by blocking acetylcholine release from the nerve termini. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Very weakly suppress the acetylcholine (ACh)-evoked current mediated by alpha-7-similar nAChRs in L.stagnalis neurons.
Catalytic activity
- a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H+
Cofactor
Note: Binds 1 Ca2+ ion.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | calcium ion binding | |
Molecular Function | calcium-dependent phospholipase A2 activity | |
Molecular Function | phospholipid binding | |
Molecular Function | toxin activity | |
Biological Process | arachidonic acid secretion | |
Biological Process | lipid catabolic process | |
Biological Process | phospholipid metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameBasic phospholipase A2 6
- EC number
- Short namessvPLA2
- Alternative names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Lepidosauria > Squamata > Bifurcata > Unidentata > Episquamata > Toxicofera > Serpentes > Colubroidea > Elapidae > Bungarinae > Bungarus
Accessions
- Primary accessionP00627
- Secondary accessions
Subcellular Location
Phenotypes & Variants
Toxic dose
LD50 is 3.6 mg/kg by intraperitoneal injection.
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | 104 | |||||
Sequence: N → T | ||||||
Natural variant | 129 | |||||
Sequence: D → N |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 2 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for propeptide, signal, chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Propeptide | PRO_0000291950 | ?-17 | ||||
Sequence: AVCVSLLGAANIPPQSL | ||||||
Signal | 1-? | |||||
Chain | PRO_0000161638 | 18-135 | Basic phospholipase A2 6 | |||
Sequence: NLYQFKNMIECAGTRTWLAYVKYGCYCGPGGTGTPLDELDRCCQTHDHCYDNAKKFGNCIPYLKTYVYTCNKPDITCTGAKGSCGRNVCDCDRAAAICFAAAPYNLANFGIDKEKHCQ | ||||||
Disulfide bond | 28↔87 | |||||
Sequence: CAGTRTWLAYVKYGCYCGPGGTGTPLDELDRCCQTHDHCYDNAKKFGNCIPYLKTYVYTC | ||||||
Disulfide bond | 42↔134 | |||||
Sequence: CYCGPGGTGTPLDELDRCCQTHDHCYDNAKKFGNCIPYLKTYVYTCNKPDITCTGAKGSCGRNVCDCDRAAAICFAAAPYNLANFGIDKEKHC | ||||||
Disulfide bond | 44↔60 | |||||
Sequence: CGPGGTGTPLDELDRCC | ||||||
Disulfide bond | 59↔115 | |||||
Sequence: CCQTHDHCYDNAKKFGNCIPYLKTYVYTCNKPDITCTGAKGSCGRNVCDCDRAAAIC | ||||||
Disulfide bond | 66↔108 | |||||
Sequence: CYDNAKKFGNCIPYLKTYVYTCNKPDITCTGAKGSCGRNVCDC | ||||||
Disulfide bond | 76↔101 | |||||
Sequence: CIPYLKTYVYTCNKPDITCTGAKGSC | ||||||
Disulfide bond | 94↔106 | |||||
Sequence: CTGAKGSCGRNVC |
Keywords
- PTM
Expression
Tissue specificity
Expressed by the venom gland.
Structure
Family & Domains
Sequence similarities
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusFragment
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length135
- Mass (Da)14,701
- Last updated2007-06-26 v3
- Checksum70D5C24E5297B2CC
Features
Showing features for non-terminal residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 1 | |||||
Sequence: A |
Mass Spectrometry
Keywords
- Technical term