O52791 · HMAS_AMYOR

Function

function

Required to synthesize hydroxyphenylglycine, a recurring skeletal component of nonproteinogenic macrocyclic peptide antibiotics such as vancomycin. Catalyzes the conversion of p-hydroxyphenylpyruvate to p-hydroxymandelate. The decarboxylation and hydroxylation activities of HmaS show novel and distinct regioselectivity, compared to all other known p-hydroxyphenylpyruvate dioxygenases, by hydroxylating the benzylic position of the substrate instead of the phenyl ring.

Catalytic activity

Cofactor

Fe cation (UniProtKB | Rhea| CHEBI:24875 )

Note: Binds 1 Fe cation per subunit.

Pathway

Antibiotic biosynthesis; vancomycin biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site161Fe cation (UniProtKB | ChEBI)
Binding site161substrate
Binding site201substrate
Binding site214substrate
Binding site241Fe cation (UniProtKB | ChEBI)
Binding site241substrate
Binding site305substrate
Binding site320Fe cation (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function4-hydroxymandelate synthase activity
Molecular Function4-hydroxyphenylpyruvate dioxygenase activity
Molecular Functioniron ion binding
Biological Processtyrosine catabolic process
Biological Processvancomycin biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-hydroxymandelate synthase
  • EC number
  • Short names
    HMS; HmaS
  • Alternative names
    • 4-hydroxyphenylpyruvate dioxygenase II

Organism names

Accessions

  • Primary accession
    O52791

Phenotypes & Variants

Chemistry

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004185341-3574-hydroxymandelate synthase

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain5-129VOC 1
Domain158-309VOC 2

Sequence similarities

Belongs to the 4HPPD family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    357
  • Mass (Da)
    38,339
  • Last updated
    1998-06-01 v1
  • Checksum
    F146FCE537CEF2CA
MQNFEIDYVEMYVENLEVAAFSWVDKYAFAVAGTSRSADHRSIALRQGQVTLVLTEPTSDRHPAAAYLQTHGDGVADIAMATSDVAAAYEAAVRAGAEAVRAPGQHSEAAVTTATIGGFGDVVHTLIQRDGTSAELPPGFTGSMDVTNHGKGDVDLLGIDHFAICLNAGDLGPTVEYYERALGFRQIFDEHIVVGAQAMNSTVVQSASGAVTLTLIEPDRNADPGQIDEFLKDHQGAGVQHIAFNSNDAVRAVKALSERGVEFLKTPGAYYDLLGERITLQTHSLDDLRATNVLADEDHGGQLFQIFTASTHPRHTIFFEVIERQGAGTFGSSNIKALYEAVELERTGQSEFGAARR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AJ223998
EMBL· GenBank· DDBJ
CAA11761.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp