O42633 · FAT1_COCHE
- ProteinVery long-chain fatty acid transport protein
- GeneFAT1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids643 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Acyl-CoA synthetase required for both the import of long chain fatty acids (LCFAs) (C14-C18) and the activation very long chain fatty acids (VLCFAs) (C20-C26) by esterification of the fatty acids into metabolically active CoA-thioesters for subsequent degradation or incorporation into phospholipids. The transport and fatty acyl-CoA synthetase activities are genetically separable and are thus independent activities. Esterifies VLCFAs in the peroxisome matrix. The VLCFAs are actively transported into peroxisomes by a PXA1-PXA2 heterodimeric transporter in the peroxisomal membrane.
Catalytic activity
- a very long-chain fatty acid + ATP + CoA = a very long-chain fatty acyl-CoA + AMP + diphosphate
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | lipid droplet | |
Cellular Component | peroxisomal membrane | |
Cellular Component | plasma membrane | |
Molecular Function | ATP binding | |
Molecular Function | long-chain fatty acid transmembrane transporter activity | |
Molecular Function | long-chain fatty acid-CoA ligase activity | |
Molecular Function | very long-chain fatty acid-CoA ligase activity | |
Biological Process | long-chain fatty acid import into cell | |
Biological Process | very long-chain fatty acid metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameVery long-chain fatty acid transport protein
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Dothideomycetes > Pleosporomycetidae > Pleosporales > Pleosporineae > Pleosporaceae > Bipolaris
Accessions
- Primary accessionO42633
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Peroxisome membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane, intramembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-15 | Cytoplasmic | ||||
Sequence: MACMHQAQLYNDLEE | ||||||
Transmembrane | 16-36 | Helical | ||||
Sequence: LLTGPSVPIVAGAAGAAALTA | ||||||
Topological domain | 37-138 | Extracellular | ||||
Sequence: YINAKYHIAHDLKTLGGGLTQSSEAIDFINRRVAQKRVLTHHIFQEQVQKQSNHPFLIFEGKTWSYKEFSEAYTRVANWLIDELDVQVGEMVAIDGGNSAEH | ||||||
Transmembrane | 139-159 | Helical | ||||
Sequence: LMLWLALDAIGAATSFLNWNL | ||||||
Topological domain | 160-249 | Cytoplasmic | ||||
Sequence: TGAGLIHCIKLCECRFVIADIDIKANIEPCRGELEETGINIHYYDPSFISSLPNNTPIPDSRTENIELDSVRGLIYTSGTTGLPKGVFIS | ||||||
Intramembrane | 250-318 | |||||
Sequence: TGRELRTDWSISKYLNLKPTDRMYTCMPLYHAAAHSLCTASVIHGGGTVVLSRKFSHKKFWPEVVASEA | ||||||
Topological domain | 319-643 | Cytoplasmic | ||||
Sequence: NIIQYVGELGRYLLNGPKSPYDRAHKVQMAWGNGMRPDVWEAFRERFNIPIIHELYAATDGLGSMTNRNAGPFTANCIALRGLIWHWKFRNQEVLVKMDLDTDEIMRDRNGFAIRCAVNEPGQMLFRLTPETLAGAPSYYNNETATQSRRITDVFQKGDLWFKSGDMLRQDAEGRVYFVDRLGDTFRWKSENVSTNEVADVMGTFPQIAETNVYGVLVPGNDGRVRSLNCHGRRRDRVDIRFAALAKHARDRLPGYAVPLFLRVTPALEYTGTLKIQKGRLKQEGIDPDKISGEDKLYWLPPGSDIYLPFGKMEWQGIVDKRIRL |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000193180 | 1-643 | Very long-chain fatty acid transport protein | |||
Sequence: MACMHQAQLYNDLEELLTGPSVPIVAGAAGAAALTAYINAKYHIAHDLKTLGGGLTQSSEAIDFINRRVAQKRVLTHHIFQEQVQKQSNHPFLIFEGKTWSYKEFSEAYTRVANWLIDELDVQVGEMVAIDGGNSAEHLMLWLALDAIGAATSFLNWNLTGAGLIHCIKLCECRFVIADIDIKANIEPCRGELEETGINIHYYDPSFISSLPNNTPIPDSRTENIELDSVRGLIYTSGTTGLPKGVFISTGRELRTDWSISKYLNLKPTDRMYTCMPLYHAAAHSLCTASVIHGGGTVVLSRKFSHKKFWPEVVASEANIIQYVGELGRYLLNGPKSPYDRAHKVQMAWGNGMRPDVWEAFRERFNIPIIHELYAATDGLGSMTNRNAGPFTANCIALRGLIWHWKFRNQEVLVKMDLDTDEIMRDRNGFAIRCAVNEPGQMLFRLTPETLAGAPSYYNNETATQSRRITDVFQKGDLWFKSGDMLRQDAEGRVYFVDRLGDTFRWKSENVSTNEVADVMGTFPQIAETNVYGVLVPGNDGRVRSLNCHGRRRDRVDIRFAALAKHARDRLPGYAVPLFLRVTPALEYTGTLKIQKGRLKQEGIDPDKISGEDKLYWLPPGSDIYLPFGKMEWQGIVDKRIRL |
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 477-525 | FACS | ||||
Sequence: DLWFKSGDMLRQDAEGRVYFVDRLGDTFRWKSENVSTNEVADVMGTFPQ |
Domain
The FACS motif is required for catalytic activity and substrate specificity.
Sequence similarities
Belongs to the ATP-dependent AMP-binding enzyme family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length643
- Mass (Da)72,311
- Last updated1998-06-01 v1
- Checksum0CAF3129C0D7B43C