O35439 · MOT1_MERUN

Function

function

Bidirectional proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, acetate and the ketone bodies acetoacetate and beta-hydroxybutyrate, and thus contributes to the maintenance of intracellular pH (By similarity).
The transport direction is determined by the proton motive force and the concentration gradient of the substrate monocarboxylate. MCT1 is a major lactate exporter (By similarity).
Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis (By similarity).
Facilitates the protonated monocarboxylate form of succinate export, that its transient protonation upon muscle cell acidification in exercising muscle and ischemic heart. Functions via alternate outward- and inward-open conformation states. Protonation and deprotonation is essential for the conformational transition (By similarity).

Catalytic activity

  • (S)-lactate(in) + H+(in) = (S)-lactate(out) + H+(out)
    This reaction proceeds in the forward
    and the backward
    directions.
  • acetate(out) + H+(out) = acetate(in) + H+(in)
    This reaction proceeds in the forward
    and the backward
    directions.
  • acetoacetate(out) + H+(out) = acetoacetate(in) + H+(in)
    This reaction proceeds in the forward
    and the backward
    directions.
  • pyruvate(out) + H+(out) = pyruvate(in) + H+(in)
  • (R)-3-hydroxybutanoate(out) + H+(out) = (R)-3-hydroxybutanoate(in) + H+(in)
    This reaction proceeds in the forward
    and the backward
    directions.
  • 3-methyl-2-oxobutanoate(out) + H+(out) = 3-methyl-2-oxobutanoate(in) + H+(in)
  • 4-methyl-2-oxopentanoate(out) + H+(out) = 4-methyl-2-oxopentanoate(in) + H+(in)
  • succinate(in) + 2 H+(in) = succinate(out) + 2 H+(out)
    This reaction proceeds in the forward direction.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site8H+ (UniProtKB | ChEBI)
Binding site12(S)-lactate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentapical plasma membrane
Cellular Componentbasolateral plasma membrane
Cellular Componentplasma membrane
Molecular Functionlactate:proton symporter activity
Molecular Functionsolute:proton symporter activity
Molecular Functionsuccinate transmembrane transporter activity
Biological Processplasma membrane lactate transport
Biological Processpyruvate transmembrane transport
Biological Processsuccinate transmembrane transport

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Monocarboxylate transporter 1
  • Short names
    MCT 1
  • Alternative names
    • Solute carrier family 16 member 1

Gene names

    • Name
      SLC16A1
    • Synonyms
      MCT1

Organism names

Accessions

  • Primary accession
    O35439

Subcellular Location

Cell membrane
; Multi-pass membrane protein
Basolateral cell membrane
; Multi-pass membrane protein
Apical cell membrane
; Multi-pass membrane protein
Note: Expression at the cell surface requires the ancillary protein BSN (By similarity).
Expression at the cell surface requires the ancillary proteins BSG and EMB (By similarity).

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane1-18Helical
Transmembrane28-48Helical
Transmembrane53-73Helical

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002113821-76Monocarboxylate transporter 1

Interaction

Subunit

Interacts with BSG; interaction mediates SLC16A1 targeting to the plasma membrane (By similarity).
Interacts with EMB; interaction mediates SLC16A1 targeting to the plasma membrane (By similarity).

Structure

Family & Domains

Sequence similarities

Keywords

Family and domain databases

Sequence

  • Sequence status
    Fragment
  • Length
    76
  • Mass (Da)
    8,269
  • Last updated
    1998-01-01 v1
  • Checksum
    736FA628B792DC25
LSILAFVDMVARPSMGLAANTKWIRPRIQYFFAASVVANGVCHLLAPLSTSYIGFCVYAGVFGFAFGWLSSVLFET

Features

Showing features for non-terminal residue.

TypeIDPosition(s)Description
Non-terminal residue1
Non-terminal residue76

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF029766
EMBL· GenBank· DDBJ
AAB84218.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp