O35439 · MOT1_MERUN
- ProteinMonocarboxylate transporter 1
- GeneSLC16A1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Bidirectional proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, acetate and the ketone bodies acetoacetate and beta-hydroxybutyrate, and thus contributes to the maintenance of intracellular pH (By similarity).
The transport direction is determined by the proton motive force and the concentration gradient of the substrate monocarboxylate. MCT1 is a major lactate exporter (By similarity).
Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis (By similarity).
Facilitates the protonated monocarboxylate form of succinate export, that its transient protonation upon muscle cell acidification in exercising muscle and ischemic heart. Functions via alternate outward- and inward-open conformation states. Protonation and deprotonation is essential for the conformational transition (By similarity).
The transport direction is determined by the proton motive force and the concentration gradient of the substrate monocarboxylate. MCT1 is a major lactate exporter (By similarity).
Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis (By similarity).
Facilitates the protonated monocarboxylate form of succinate export, that its transient protonation upon muscle cell acidification in exercising muscle and ischemic heart. Functions via alternate outward- and inward-open conformation states. Protonation and deprotonation is essential for the conformational transition (By similarity).
Catalytic activity
- (S)-lactate(in) + H+(in) = (S)-lactate(out) + H+(out)This reaction proceeds in the forward and the backward directions.(S)-lactate (in)CHEBI:16651
+ H+ (in)CHEBI:15378= (S)-lactate (out)CHEBI:16651+ H+ (out)CHEBI:15378 - acetate(out) + H+(out) = acetate(in) + H+(in)This reaction proceeds in the forward and the backward directions.
- acetoacetate(out) + H+(out) = acetoacetate(in) + H+(in)This reaction proceeds in the forward and the backward directions.
- pyruvate(out) + H+(out) = pyruvate(in) + H+(in)
- (R)-3-hydroxybutanoate(out) + H+(out) = (R)-3-hydroxybutanoate(in) + H+(in)This reaction proceeds in the forward and the backward directions.
- 3-methyl-2-oxobutanoate(out) + H+(out) = 3-methyl-2-oxobutanoate(in) + H+(in)
- 4-methyl-2-oxopentanoate(out) + H+(out) = 4-methyl-2-oxopentanoate(in) + H+(in)
- succinate(in) + 2 H+(in) = succinate(out) + 2 H+(out)This reaction proceeds in the forward direction.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | apical plasma membrane | |
Cellular Component | basolateral plasma membrane | |
Cellular Component | plasma membrane | |
Molecular Function | lactate:proton symporter activity | |
Molecular Function | solute:proton symporter activity | |
Molecular Function | succinate transmembrane transporter activity | |
Biological Process | plasma membrane lactate transport | |
Biological Process | pyruvate transmembrane transport | |
Biological Process | succinate transmembrane transport |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameMonocarboxylate transporter 1
- Short namesMCT 1
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Gerbillinae > Meriones
Accessions
- Primary accessionO35439
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Basolateral cell membrane ; Multi-pass membrane protein
Apical cell membrane ; Multi-pass membrane protein
Note: Expression at the cell surface requires the ancillary protein BSN (By similarity).
Expression at the cell surface requires the ancillary proteins BSG and EMB (By similarity).
Expression at the cell surface requires the ancillary proteins BSG and EMB (By similarity).
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 1-18 | Helical | ||||
Sequence: LSILAFVDMVARPSMGLA | ||||||
Transmembrane | 28-48 | Helical | ||||
Sequence: IQYFFAASVVANGVCHLLAPL | ||||||
Transmembrane | 53-73 | Helical | ||||
Sequence: IGFCVYAGVFGFAFGWLSSVL |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000211382 | 1-76 | Monocarboxylate transporter 1 | |||
Sequence: LSILAFVDMVARPSMGLAANTKWIRPRIQYFFAASVVANGVCHLLAPLSTSYIGFCVYAGVFGFAFGWLSSVLFET |
Interaction
Subunit
Interacts with BSG; interaction mediates SLC16A1 targeting to the plasma membrane (By similarity).
Interacts with EMB; interaction mediates SLC16A1 targeting to the plasma membrane (By similarity).
Interacts with EMB; interaction mediates SLC16A1 targeting to the plasma membrane (By similarity).
Structure
Family & Domains
Sequence similarities
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusFragment
- Length76
- Mass (Da)8,269
- Last updated1998-01-01 v1
- Checksum736FA628B792DC25
Features
Showing features for non-terminal residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 1 | |||||
Sequence: L | ||||||
Non-terminal residue | 76 | |||||
Sequence: T |