N4VG36 · NIS1_COLOR

Function

function

Secreted effector that induces necrotic lesions in Nicotiana benthamiana (PubMed:22352720).
Interacts with the host receptor-like kinases (RLKs) BAK1/SERK3 and BKK1/SERK4, inhibits their kinase activity and suppresses INF1-induced pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) in N.benthamiana (PubMed:30584105).
Also interacts with the host receptor-like cytoplasmic kinase (RLCK) BIK1 and inhibits its kinase activity, thereby inhibiting PAMP-induced ROS generation (PubMed:30584105).
In PTI, phosphorylation relaying by RLKs and RLCKs is critical for the initiation of downstream signaling (PubMed:30584105).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentextracellular region
Cellular Componenthost cell cytoplasm

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Necrosis-inducing secreted protein 1
  • Alternative names
    • Secreted effector NIS1

Gene names

    • Name
      NIS1
    • ORF names
      Cob_01067, Cob_v001680

Organism names

Accessions

  • Primary accession
    N4VG36
  • Secondary accessions
    • H7CE97

Proteomes

Subcellular Location

Secreted
Host cytoplasm
Note: Secreted, via the endoplasmic reticulum-to-Golgi route and subsequent exocytosis, and accumulates in a ring-like region around the neck of the biotrophic primary hyphae at the pathogen-plant interface.

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis125Specifically reduces the interaction with N.benthamiana SERK3 and its paralogs but not Arabidopsis BAK1.

PTM/Processing

Features

Showing features for signal, chain, glycosylation.

TypeIDPosition(s)Description
Signal1-19
ChainPRO_500412312520-162Necrosis-inducing secreted protein 1
Glycosylation88N-linked (GlcNAc...) asparagine
Glycosylation126N-linked (GlcNAc...) asparagine
Glycosylation133N-linked (GlcNAc...) asparagine
Glycosylation150N-linked (GlcNAc...) asparagine

Keywords

PTM databases

Expression

Induction

Preferentially expressed in biotrophic invasive hyphae at the post-invasive stage.

Interaction

Subunit

Interacts with the host pattern recognition receptor (PRR)-associated kinases BAK1/SERK3, BKK1/SERK4 and BIK1.

Protein-protein interaction databases

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region103-132BAK1/SERK3-binding

Domain

The C-terminal region from residues 103 to 132 is important for BAK1/SERK3-binding and the host cell death suppression.

Sequence similarities

Belongs to the NIS1 effector family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    162
  • Mass (Da)
    16,796
  • Last updated
    2013-06-26 v1
  • Checksum
    DCC17B3BE1DBF39B
MQFLTSLAAAASLVSLASARISGIALPQTVKAGDNINAIVVTEGYIQSVQDIAIAFGVAPAASAYPGTLSTLLGSFYLGPEQSNVQNNITEPITIPESLVPGEYVIAASLFSLYGASSSPTVSNYNVTVNVGNETSTTYVRSQFYVGNSNSTVCLGGYTRKI

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB669517
EMBL· GenBank· DDBJ
BAL70334.1
EMBL· GenBank· DDBJ
Genomic DNA
KB725990
EMBL· GenBank· DDBJ
ENH80006.1
EMBL· GenBank· DDBJ
Genomic DNA
AMCV02000002
EMBL· GenBank· DDBJ
TDZ25263.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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