M2UJ60 · TPCC_COCH5
- ProteinCytochrome P450 monooxygenase tpcC
- GenetpcC
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids525 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Cytochrome P450 monooxygenase; part of the gene cluster that mediates the biosynthesis of terpestacin (PubMed:29185768).
The bifunctional terpene synthase tpcA converts isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) into the sesterterpene preterpestacin I (PubMed:29185768).
The C-terminal prenyltransferase (PT) domain of tpcA catalyzes formation of GFPP, whereas the N-terminal terpene cyclase (TC) domain catalyzes the cyclization of GFPP into preterpestacin I (PubMed:29185768).
The cytochrome P450 monooxygenase tpcB then hydroxylates preterpestacin I to yield 24-hydroxypreterpstacin I (renamed as preterpestacin II) whereas the cytochrome P450 monooxygenase tpcC further hydroxylates preterpestacin II to yield 16,17-dihydroxypreterpestacin II (renamed as preterpestacin III) (PubMed:29417814).
Finally, the FAD-dependent monooxygenase tpcD converts preterpestacin III into terpestacin (PubMed:29417814).
The bifunctional terpene synthase tpcA converts isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) into the sesterterpene preterpestacin I (PubMed:29185768).
The C-terminal prenyltransferase (PT) domain of tpcA catalyzes formation of GFPP, whereas the N-terminal terpene cyclase (TC) domain catalyzes the cyclization of GFPP into preterpestacin I (PubMed:29185768).
The cytochrome P450 monooxygenase tpcB then hydroxylates preterpestacin I to yield 24-hydroxypreterpstacin I (renamed as preterpestacin II) whereas the cytochrome P450 monooxygenase tpcC further hydroxylates preterpestacin II to yield 16,17-dihydroxypreterpestacin II (renamed as preterpestacin III) (PubMed:29417814).
Finally, the FAD-dependent monooxygenase tpcD converts preterpestacin III into terpestacin (PubMed:29417814).
Cofactor
Pathway
Secondary metabolite biosynthesis; terpenoid biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | monooxygenase activity | |
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | |
Biological Process | terpenoid biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCytochrome P450 monooxygenase tpcC
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Dothideomycetes > Pleosporomycetidae > Pleosporales > Pleosporineae > Pleosporaceae > Bipolaris
Accessions
- Primary accessionM2UJ60
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 13-33 | Helical | ||||
Sequence: LPVTLVSLLVGSIFYFCYLTV |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000453716 | 1-525 | Cytochrome P450 monooxygenase tpcC | |||
Sequence: MAILSSALVTIDLPVTLVSLLVGSIFYFCYLTVYRLFLSPIAHFPGPKLAAWTYWYEFWYDVIAEPEYTFKIGRLHKIYGPVVRINPDEIHIADPDFYDTIYAGSGRKRDKWDWITRSFGVDESLIGTLKHDEHRVRRASLSPYFSKQSVRALQPLIDRNMAILLDRLREFAKSGSPLKLDDAYAALTNDIVEDYAFGRSDHRLEAPDFDPSFRDAMLQGGKAGHVLKHFTWLMDLLKKLPDSLLLKLSPAMGAYSQLQTSVKRQVAEIQHAHQMHTYDKTRRTIFHEILNSKLSDYDKSTDRLWQEGEVVVAAGTITTAWALGVSTYFVLATPDILRKLKTELEAAIPDPSQPLNLITLEALPFLTGVVQEGVRLSHAISHRLHRICPDETLIYQDNDNQREWRIPPGTPLSMTSNLVHHDERVFPDSHAFKPERWLDNARLERYLVSFGKGGRACLGINLAYAELYLTLAALFRVYGTEQVKGKDDVGTLQLFETSAADLVITSDTVVPVMPEDSKGLRVKVS |
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length525
- Mass (Da)59,551
- Last updated2013-05-01 v1
- ChecksumCF77B69DF691CDBF
Keywords
- Technical term