M2UJ60 · TPCC_COCH5

Function

function

Cytochrome P450 monooxygenase; part of the gene cluster that mediates the biosynthesis of terpestacin (PubMed:29185768).
The bifunctional terpene synthase tpcA converts isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) into the sesterterpene preterpestacin I (PubMed:29185768).
The C-terminal prenyltransferase (PT) domain of tpcA catalyzes formation of GFPP, whereas the N-terminal terpene cyclase (TC) domain catalyzes the cyclization of GFPP into preterpestacin I (PubMed:29185768).
The cytochrome P450 monooxygenase tpcB then hydroxylates preterpestacin I to yield 24-hydroxypreterpstacin I (renamed as preterpestacin II) whereas the cytochrome P450 monooxygenase tpcC further hydroxylates preterpestacin II to yield 16,17-dihydroxypreterpestacin II (renamed as preterpestacin III) (PubMed:29417814).
Finally, the FAD-dependent monooxygenase tpcD converts preterpestacin III into terpestacin (PubMed:29417814).

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Pathway

Secondary metabolite biosynthesis; terpenoid biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site457Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentmembrane
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functionmonooxygenase activity
Molecular Functionoxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cytochrome P450 monooxygenase tpcC
  • EC number
  • Alternative names
    • Terpestacin biosynthesis cluster protein C

Gene names

    • Name
      tpcC
    • ORF names
      COCHEDRAFT_1212392

Organism names

Accessions

  • Primary accession
    M2UJ60

Proteomes

Subcellular Location

Membrane
; Single-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane13-33Helical

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004537161-525Cytochrome P450 monooxygenase tpcC

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    525
  • Mass (Da)
    59,551
  • Last updated
    2013-05-01 v1
  • Checksum
    CF77B69DF691CDBF
MAILSSALVTIDLPVTLVSLLVGSIFYFCYLTVYRLFLSPIAHFPGPKLAAWTYWYEFWYDVIAEPEYTFKIGRLHKIYGPVVRINPDEIHIADPDFYDTIYAGSGRKRDKWDWITRSFGVDESLIGTLKHDEHRVRRASLSPYFSKQSVRALQPLIDRNMAILLDRLREFAKSGSPLKLDDAYAALTNDIVEDYAFGRSDHRLEAPDFDPSFRDAMLQGGKAGHVLKHFTWLMDLLKKLPDSLLLKLSPAMGAYSQLQTSVKRQVAEIQHAHQMHTYDKTRRTIFHEILNSKLSDYDKSTDRLWQEGEVVVAAGTITTAWALGVSTYFVLATPDILRKLKTELEAAIPDPSQPLNLITLEALPFLTGVVQEGVRLSHAISHRLHRICPDETLIYQDNDNQREWRIPPGTPLSMTSNLVHHDERVFPDSHAFKPERWLDNARLERYLVSFGKGGRACLGINLAYAELYLTLAALFRVYGTEQVKGKDDVGTLQLFETSAADLVITSDTVVPVMPEDSKGLRVKVS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KB445573
EMBL· GenBank· DDBJ
EMD93706.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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