K2HL09 · K2HL09_9RHOB
- ProteintRNA-dihydrouridine synthase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids334 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines.
Catalytic activity
- a 5,6-dihydrouridine in tRNA + NAD+ = a uridine in tRNA + NADH + H+
- a 5,6-dihydrouridine in tRNA + NADP+ = a uridine in tRNA + NADPH + H+
Cofactor
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 30-32 | FMN (UniProtKB | ChEBI) | ||||
Sequence: PMA | ||||||
Binding site | 83 | FMN (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Active site | 113 | Proton donor | ||||
Sequence: C | ||||||
Binding site | 152 | FMN (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 182 | FMN (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 237-238 | FMN (UniProtKB | ChEBI) | ||||
Sequence: GR |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | RNA binding | |
Molecular Function | tRNA dihydrouridine synthase activity |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-dihydrouridine synthase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Rhodobacterales > Roseobacteraceae > Oceaniovalibus
Accessions
- Primary accessionK2HL09
Proteomes
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 27-301 | DUS-like FMN-binding | ||||
Sequence: LLAPMAGITDLPFRNIVAGFGAGLVTSEMVASDALNGGSAATIAKVSGAGRQDTSVQIAGRQPAEMTEAARRVADAGARIVDINMGCPAKRVTGGLSGSALMRDPDHALRLIDAVARAVAVPVTLKMRLGWDGDCLTAPQIARRAEAAGIRMVTVHGRTRCQFYKGRADWTAIRAVADAVTIPVVANGDICDAADARAALAASGAQAVMVGRGAQGRPWALAEIGAALAGADTPPVPADLTALALAHLDAARSFYGADLGARTMRKHLGWYMDHA |
Sequence similarities
Belongs to the dus family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length334
- Mass (Da)34,286
- Last updated2012-11-28 v1
- Checksum0EB190BB7BA1C172
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AMGO01000052 EMBL· GenBank· DDBJ | EKE43584.1 EMBL· GenBank· DDBJ | Genomic DNA |