J7Q6V7 · J7Q6V7_METSZ
- ProteintRNA (cytidine(34)-2'-O)-methyltransferase
- GenetrmL
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids158 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide.
Catalytic activity
- 5-carboxymethylaminomethyluridine34 in tRNA(Leu) + S-adenosyl-L-methionine = 5-carboxymethylaminomethyl-2'-O-methyluridine34 in tRNA(Leu) + S-adenosyl-L-homocysteine + H+
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 87 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Binding site | 109 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 129 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: I | ||||||
Binding site | 137 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | RNA binding | |
Molecular Function | tRNA (5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase activity | |
Molecular Function | tRNA (cytidine(34)-2'-O)-methyltransferase activity | |
Biological Process | wobble position cytosine ribose methylation | |
Biological Process | wobble position uridine ribose methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA (cytidine(34)-2'-O)-methyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Methylocystaceae > Methylocystis
Accessions
- Primary accessionJ7Q6V7
Proteomes
Subcellular Location
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 4-148 | tRNA/rRNA methyltransferase SpoU type | ||||
Sequence: LTLALYQPDIPQNAGAMLRTCACLGVSAAIIGPAGFPTSDRDFRRAGMDYLDHVTIDRHGSFAAFQAWRAASGQAGESRRLLLLSTRAAGSYLDCDYRAGDILLVGRESAGAPEEVHAAADLALRIPMRPRMRSLNVAVAAAMVM |
Sequence similarities
Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. TrmL subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length158
- Mass (Da)16,888
- Last updated2012-10-31 v1
- ChecksumE19D0B65E6618AB9
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
HE956757 EMBL· GenBank· DDBJ | CCJ05547.1 EMBL· GenBank· DDBJ | Genomic DNA |