H8WGT9 · H8WGT9_ECTFE
- ProteinGDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase
- Genealg11
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids310 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
GDP-Man:Man3GlcNAc2-PP-Dol alpha-1,2-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes, on the cytoplasmic face of the endoplasmic reticulum, the addition of the fourth and fifth mannose residues to the dolichol-linked oligosaccharide chain, to produce Man5GlcNAc2-PP-dolichol core oligosaccharide. Man5GlcNAc2-PP-dolichol is a substrate for ALG3, the following enzyme in the biosynthetic pathway.
Catalytic activity
- an alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + 2 GDP-alpha-D-mannose = an alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + 2 GDP + 2 H+This reaction proceeds in the forward direction.
an α-D-Man-(1→3)-[α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol RHEA-COMP:19515 + 2 CHEBI:57527 = an α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol RHEA-COMP:19516 + 2 CHEBI:58189 + 2 CHEBI:15378
Pathway
Protein modification; protein glycosylation.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Molecular Function | GDP-Man:Man3GlcNAc2-PP-Dol alpha-1,2-mannosyltransferase activity | |
Biological Process | protein N-linked glycosylation |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Porifera > Demospongiae > Heteroscleromorpha > Axinellida > Raspailiidae > Raspailiinae > Ectyoplasia
Accessions
- Primary accessionH8WGT9
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Single-pass membrane protein
Keywords
- Cellular component
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-190 | ALG11 mannosyltransferase N-terminal | ||||
Sequence: RVLWNSIQALQRRYSFVQCVVYTGDRGASADEILAKVQQRFGLYLANNVQFVYLRLRCAVEAHRWPRFTLLGQSLGSMLLGLEALLKFCPNVYIDSMGYAFTLPLFRWIGGCKTASYVHYPVVSQDMLEKVSEQVSSFNNPSWISRSKILTKFKIIYYKLFAKIYGFAGRRNDVIMVNSSWTHGHIERIW | ||||||
Domain | 214-309 | Glycosyl transferase family 1 | ||||
Sequence: HKSRSLRIISVGQFRPEKDHKMQIRVLQRLLTALSRKQRQGVMLVLVGSCRNSEDQARVEELKQYAKQLGVGDRVLFRINVSYQELQDELCQASVA |
Sequence similarities
Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusFragment
- Length310
- Mass (Da)35,859
- Last updated2012-05-16 v1
- Checksum8FF508FB69338F17
Features
Showing features for non-terminal residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 1 | |||||
Sequence: R | ||||||
Non-terminal residue | 310 | |||||
Sequence: I |