H2E7Q6 · AAMA2_GALM3

Function

function

Major toxin belonging to the bicyclic octapeptides amatoxins that acts by binding non-competitively to RNA polymerase II and greatly slowing the elongation of transcripts from target promoters (PubMed:22202811, PubMed:7642577, PubMed:8702941).

Miscellaneous

The typical symptoms of amatoxin poisoning are gastro-intestinal distress beginning 6-12 hours after ingestion, a remission phase lasting 12-24 hours, and progressive loss of liver function culminating in death within 3-5 days (PubMed:12475187).
One of the few effective treatments is liver transplantation (PubMed:12475187).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Molecular Functiontoxin activity

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Alpha-amanitin proprotein 2
  • Cleaved into 1 chains
    • Alpha-amanitin
      Alternative names: Amatoxin
      , Gamma-amanitin

Gene names

    • Name
      AMA1-2
    • ORF names
      GALMADRAFT_218055

Organism names

  • Taxonomic identifier
  • Strain
    • CBS 339.88
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Basidiomycota > Agaricomycotina > Agaricomycetes > Agaricomycetidae > Agaricales > Agaricineae > Strophariaceae > Galerina

Accessions

  • Primary accession
    H2E7Q6
  • Secondary accessions
    • A0A067TV11

Proteomes

PTM/Processing

Features

Showing features for propeptide, modified residue, peptide, cross-link.

TypeIDPosition(s)Description
PropeptidePRO_00004435671-10
Modified residue11(3R,4R)-4,5-dihydroxyisoleucine; in form alpha-amanitin
Modified residue11(3R,4S)-4-hydroxyisoleucine; in form gamma-amanitin
PeptidePRO_000044356811-18Alpha-amanitin
Cross-link11↔18Cyclopeptide (Ile-Pro)
Cross-link12↔162'-cysteinyl-6'-hydroxytryptophan sulfoxide (Trp-Cys)
Modified residue184-hydroxyproline
PropeptidePRO_000044356919-35

Post-translational modification

Processed by the macrocyclase-peptidase enzyme POPB to yield a toxic bicyclic octapeptide (By similarity).
POPB first removes 10 residues from the N-terminus (By similarity).
Conformational trapping of the remaining peptide forces the enzyme to release this intermediate rather than proceed to macrocyclization (By similarity).
The enzyme rebinds the remaining peptide in a different conformation and catalyzes macrocyclization of the N-terminal 8 residues (By similarity).

Keywords

Family & Domains

Sequence similarities

Belongs to the MSDIN fungal toxin family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    35
  • Mass (Da)
    3,892
  • Last updated
    2012-03-21 v1
  • Checksum
    A49ED273AEFC7716
MFDTNSTRLPIWGIGCNPWTAEHVDQTLVSGNDIC

Sequence caution

The sequence KDR86192.1 differs from that shown. Reason: Erroneous gene model prediction

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JN827312
EMBL· GenBank· DDBJ
AEX26936.1
EMBL· GenBank· DDBJ
mRNA
KL142367
EMBL· GenBank· DDBJ
KDR86192.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.

Similar Proteins

Disclaimer

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