H2B6U5 · H2B6U5_9TELE

Function

function

Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends.

Catalytic activity

  • S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.
    EC:2.3.2.27 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 1 divalent metal cation per subunit. Mg2+ or Mn2+.

GO annotations

AspectTerm
Cellular ComponentDNA recombinase complex
Cellular Componentendodeoxyribonuclease complex
Cellular Componentnucleus
Molecular Functiondouble-stranded DNA endonuclease activity
Molecular Functionhistone binding
Molecular Functionprotein homodimerization activity
Molecular Functionsequence-specific DNA binding
Molecular Functionubiquitin protein ligase activity
Molecular Functionzinc ion binding
Biological Processadaptive immune response
Biological Processchromatin organization
Biological Processpre-B cell allelic exclusion
Biological ProcessT cell differentiation in thymus
Biological ProcessV(D)J recombination

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    V(D)J recombination-activating protein 1
  • EC number

Gene names

    • Name
      RAG1

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Characiformes > Characoidei > Alestidae > Brycinus

Accessions

  • Primary accession
    H2B6U5

Subcellular Location

Keywords

  • Cellular component

Interaction

Subunit

Homodimer.

Family & Domains

Sequence similarities

Belongs to the RAG1 family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Fragment
  • Length
    419
  • Mass (Da)
    47,742
  • Last updated
    2012-03-21 v1
  • Checksum
    74AB4E01C1A1CEFA
VSTSWDVGIIDGLSGWTASVDEIPANTISRRFRYDVALASTLKDLEEDIMEGLKERGLDDSTCTSGFTVVIKESCDGMGDVSEKHGGGPAIPEKAVRFSFTLMSITVQAEGEEEAVTIFQEQKPNSELSCRPMCLMFVDESDHETLTAILGPVVAERKAMKESRLILSIGGLLRSFRFYFRGTGYDEKMVREMEGLEASGSTYVCTLCDSTRAEASQNMVLHSITRSHEENLERYEIWRTNPYSESAEELRDRVKGVSAKPFMETRTTLDALHCDIGNATEFYKIFQDEIGEMYLKNNPTREERRCRRSALDKQLRMKEKLKPVLRTNGKYAGGLKTRGAVEVVFEMVRSSERRKAEMEQTEPYLQNHPMWRCTTPARNCPDQLRRFHFNFQKFAEIRSTTFKYGYDGKKSNYLHKTLG

Features

Showing features for non-terminal residue.

TypeIDPosition(s)Description
Non-terminal residue1
Non-terminal residue419

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
HQ289290
EMBL· GenBank· DDBJ
AEQ02561.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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